两个品种水稻对砷的吸收富集与转化特征及其健康风险

30420104ActaScientiaeCircumstantiaeVol.30,No.4Apr.,2010:(No.2006BAJ05A08);(973)(No.2008CB418201)SupportedbytheNationalKeyTechnologiesR&DProgramofChinaduringthe11thFive2YearPlanPeriod(No.2006BAJ05A08)andtheMajorStateBasicResearchDevelopmentProgramofChina(973)(No.2008CB418201):(1984),;3(),Tel:[phone],E2mail:[e-mail]:WANGLingmei(1984),female;3Correspondingauthor,Tel:[phone],E2mail:[e-mail],,,.2010.[J].,30(4):832-840WangLM,WeiCY,YangLS,etal.2010.Arsenicaccumulationandspeciationintworicevarietiesandrelatedhealthrisks[J].ActaScientiaeCircumstantiae,30(4):832-8401,2,1,3,1,31.,1001012.,1000493.,100085:2009206224:2009207202:2010201219:2402416,(0102040mgkg-1)(,11),.,,416402.,,;,4160.062mgkg-1,4020.278mgkg-1.(10mgkg-1),.,,,60%40%.WHO,416402.:;;;;:025322468(2010)042832209:X144:AArsenicaccumulationandspeciationintworicevarietiesandrelatedhealthrisksWANGLingmei1,2,WEIChaoyang1,3,YANGLinsheng1,DUANGuilan31.InstituteofGeographicSciencesandNaturalResourcesResearch,ChineseAcademyofSciences,Beijing1001012.GraduateUniversityofChineseAcademyofSciences,Beijing1000493.ResearchCenterforEco2environmentalSciences,ChineseAcademyofSciences,Beijing100085Received24June2009;receivedinrevisedform2July2009;accepted19January2010Abstract:Arsenicistoxicandcarcinogenic,soconsumptionofarsenicaccumulatedricemayproduceserioushealthrisks.Ricevarieties(OryzasativaL.)thataccumulatetheleastarsenic,especiallyinthericegrain,aredesired.Tworicevarieties,namedWeiyou402(WY)andEryou416(EY)wereselectedtogrowinarsenicspikedsoils(0,10,20and40mgkg-1arsenic)andnaturalarsenicsoils(11ofgeogenichighlevelarsenicsoilfromGuizhouprovinceandsiltloamsoilfromBeijing)inagreenhouse,tocomparetheirarsenicuptake,accumulation,speciationandrelatedhealthrisks.Arsenicwasfoundtogreatlyinhibitthegrowthofthetworicevarieties,includingthebiomassofshootsandroots,plantheightandthenumberofspikes,however,EYwaslessaffectedthanWYbythearsenictreatments.Arsenicinricegrainincreasedwiththeadditionofarsenicinsoils,bothinWYandEY,withnodifferencebetweenthetwovarieties;however,innaturalarsenicsoils,EYaccumulatedsignificantlylessarsenicinricegrainthanthatofWY,withaveragearseniccontentsmeasuredas0.062and0.278mgkg-1forEYandWY,respectively.Underlowarsenictreatment(10mgkg-1),morearsenicwastranslocatedfromrootstostalksandleaves,whereasnochangeswerefoundfortheseinotherarsenictreatments.Theconstitutionandproportionofarsenicspeciesindifferentpartsofthetworicevarietiesweresimilar,onlynon2organicarsenateandarsenitewerefoundinroots,stalksandleaves,whileinthericegrainsorganicarsenic,includingMMAandDMA,occupied40%ofthetotalarsenic.WesuggestplantingEYriceinsteadofWY4:inminingareasinordertoreducethehealthrisksofarsenic.Keywords:arsenate;arsenite;MMA;DMA;pottrials1(Introduction),,(Meharg,2004;Smithetal.,1998).,;2(Mehargetal.,2003a;2003b;,2004).,;,,,,.402416.,.2(Materialsandmethods)2.1(6.81mgkg-1)(257mgkg-1).,;(Na2HAsO47H2O),()10mgkg-120mgkg-140mgkg-14.,,11,.1.,21cm,24cm.5kg;,;N110g,P2O50.75g,K2O1.0g;,20.1Table1ThephysicalandchemicalpropertiesofsoilspH(CaCl2)/(gkg-1)/(mgkg-1)/(gkg-1)/(mgkg-1)/(mgkg-1)7.5913.10.41%59.64%39.95%6.811.010.195.8a6.9921.828.23%62.16%9.60%131.91.513.2138.9:a.2.2200849.402,;416,,2004,.,30%15min,.4,,6.8:00am22:00pm,260350Em-2s-1;28C,20C;60%70%.1/41,(Zhuetal.,1999);0.1molL-1KOHHClpH5.5(Trostleetal.,2001).,;2,1,4.,2cm.2.310,.,;34cm,.,,.,,,.23cm,,50;,.33830.,,,,.2.42.4.1.+(V/V=101),(HG2AFS).(GBW07604).2.4.220mgkg-1.(TFA)(Abedinetal.,2002b),HPLC2DRC2ICP2MS4,.2.5SPSS13,;,20mgkg-1.t.3(Results)3.1,,,.1,402416;,416402;,2.,().,3(2).(p0.05),(p0.001).,416402.416;40220mgkg-1,,40mgkg-1,,50%(2).(p0.001),40mgkg-1,41640249.6%71.0%.,416402(p0.001),46%(2).,().(20mgkg-1)402416,(40mgkg-1),(p0.05).,(40mgkg-1),4024166513%45.6%.,4164021/3(2).,.40210mgkg-120mgkg-1,.41610mgkg-1,,20mgkg-140mgkg-1(2).,(40mgkg-1),40%(2).3.2,,;,4021/541,1/40,1/24;4161/610,1/18,1/23.,.,40243830416.402(40mgkg-1)15,329mgkg-1;41612,216mgkg-1;4024161.5(1a).1(:(p0.05);(p0.05),n=4.(131.9mgkg-1))Fig.1Effectofarsenicontheaccumulationofarsenicinriceroots,stalks,leavesandgrain(barsaremeansandstandarderrorofmeans,n=4,thedarkandgraybarsrepresentRiceEryou416andWeiyou402,respectively)0.7712.00mgkg-1,40mgkg-1,40241630%(1b).0.4220.19mgkg-1,;40mgkg-1,4164024.711.2.,,416402,4024164(1c).,,4020.1120.498mgkg-1,4160.0680.450mgkg-1.,4160.062mgkg-1,4020.278mgkg-1(p0.05),(1d).3.3,(,2005).,(2).(10mgkg-1);,.,4166384:402,,;402416;10mgkg-1,402416,20mgkg-1,(2).,(F012652.135,p0.1540.610);(F37.962,p0.000).2Fig.2Thevaluesoftranslocationfactorsinricestalks,leavesandgrain(thesolidanddashedlinesrepresentRiceEryou416andWeiyou402,respectively)3.420mgkg-1,.,As();As(),As(V).DMA()MMA().,40%,DMAMMA25.96%13.66%(3).3()(a.416;b.402)Fig.3Arsenicspeciationindifferentricetissues(roots,stalks,leavesandgrain)usingtrifluoroaceticacid(TFA)extraction(a.RiceEryou416;b.RiceWeiyou402)4(Discussion)4.1.,40mgkg-1,73830,,56%,().(,,),,.Rahman(2008),60mgkg-1(),.,,,.416.40210mgkg-1,,.(10mgkg-1),402,(2).(2006)(2008).As()(P),,As()P,P,P,(Jac