Sequin帮助文件生物软件网()提供翻译:qshchen@sohu.com1Sequin帮助文件目录•介绍(Introduction)o帮助文档(Thehelpdocumentation)o表格的组织(OrganizationofForms)oSequin总览(OverviewofSequin)•“Sequin欢迎表格”(WelcometoSequinForm)•提交者表格(SubmisstionAuthorsPage)o提交页(SubmisstionPage)我们是否可以在发表前公布此记录?(Maywereleasethisrecordbeforepublication?)稿件的临时标题(Tentativetitleformanuscript)o联系页(ContactPage)o作者页(AuthorsPage)o机构页(AffiliationPage)•“序列格式”表格(SequenceFormatForm)o提交类型Submissiontypeo序列数据格式Sequencedataformato提交类别SubmissionCategory•物种和测序格式OrganismandSequencesFormo物种页OrganismPage物种Organism测序部位LocationofSequence翻译遗传密码GeneticCodeforTranslation:o核酸页NucleotidePage分子Molecule拓扑结构Topology5'末端未完成/3'末端未完成Incompleteat5'end/Incompleteat3'endFASTA定义行(以序列号起始)FASTAdeflinestartswithsequenceID核酸序列的FASTA格式FASTAformatfornucleotidesequences核酸的定义行(标题)NucleotideDefinitionline(title)核酸FASTA格式的第一行的例子ExampleofthefirstlineofanucleotideFASTAsequence核酸序列对比的FASTA+GAP格式FASTA+GAPformatforalignednucleotidesequences以PHYLIP格式输出的核酸序列对比PHYLIPformatforalignednucleotidesequences以NEXUS格式输出的核酸序列对比NEXUSformatforalignednucleotidesequences对PHYLIP和NEXUS的一些补充信息SourceModifiersforPHYLIPandNEXUS导入系列对比序列片段Importingalignedsetsofsegmentedsequences向Sequin导入核酸序列ToimportanucleotidesequenceintoSequin向Sequin导入一系列核酸序列ToimportasetofnucleotidesequencesintoSequino蛋白质页ProteinPage由蛋白测序确认的概念性翻译ConceptualtranslationconfirmedbypeptidesequencingN-端不完整/C-端不完整IncompleteatNH3end/IncompleteatCO2endFASTA定义行(以序列号起始)FASTAdeflinestartswithsequenceID建立有CDS间隔的原始mRNACreateinitialmRNAwithCDSintervals蛋白序列的FASTA格式FASTAformatforproteinsequences蛋白质的定义行(标题)ProteinDefinitionline(title)蛋白质FASTA格式的第一行的例子ExampleofthefirstlineofaproteinFASTA2sequence:向Sequin导入蛋白序列ToimportaproteinsequenceintoSequin向Sequin导入一系列蛋白序列ToimportasetofproteinsequencesintoSequino序列导入确认窗口SequenceImportCheckWindowo注解页AnnotationPage对一系列序列增加基因、rRNA和CDS属性AddingGene,rRNAandCDSfeaturestoasetofsequences对一系列序列添加标题Addingatitletoasetofsequences•来源限定词表格SourceModifiersForm•组配跟踪AssemblyTracking•编辑记录/档案EditingtheRecordo概述Overviewo向数据库提交昀终记录Submittingthefinishedrecordtothedatabase•记录阅览器TheRecordViewero目标序列TargetSequenceo显示格式DisplayFormat摘要Summary图形Graphic队列Alignment序列SequenceGenBankEMBLFASTAASN.1桌面Desktopo完成Doneo屏幕下端的控制按钮Controlsonthebottomofthescreen•描述符Descriptorso概述Overviewo注释菜单Annotatemenu更新数据Updatedate生成数据CreatedateGenBankblock领域Region评述Comment标题Title分子描述Moleculedescription分子Molecule完整性Completedness技术方法Technique分类Class:分子构象Topology:链Strand:生物学来源BiologicalSource•特征Featureso概述Overviewo注释菜单Annotatemenu特性页PropertiesPage常规子页GeneralSubpage评述子页CommentSubpage引用子页CitationsSubpage3参照子页Cross-refsSubpage定位页Locationpageo衰减子attenuatoroC_区C_regionoCAAT_信号CAAT_signalo编码区CDS编码区页CodingRegionPage常规子页GeneralSubpage其它选项子页ExceptionsSubpage蛋白质子页ProteinSubpage产物子页ProductSubpageo冲突conflictoD-环D-loopoD_片段D_segmento增强子enhancero外显子exonoGC_信号GC_signalo基因geneo干涉DNAiDNAo内含子intronoJ_片段J_segmento长终止重复LTRo成熟肽mat_peptideomisc_bindingomisc_differenceomisc_featureomisc_recombomisc_RNAomisc_signalomisc_structureo修饰的碱基modified_baseomRNAoN_区N_regiono原序列old_sequenceo加尾信号polyA_signalopolyA_位点polyA_siteo前体RNAprecursor_RNAo初级转录本prim_transcripto引物结合位点primer_bindo启动子promotero蛋白质结合位点protein_bindo核糖体结合位点RBSo重复区repeat_regiono重复单元repeat_unito复制原点rep_originorRNAo转换区S_regiono卫星序列satelliteo细胞质小分子RNAscRNAo信号肽sig_peptideo核内小分子RNAsnRNAo核仁小分子RNAsnoRNA4o来源sourceo茎环结构stem_loopo序列标签位点STSoTATA_信号TATA_signalo终止子terminatoro转运肽transit_peptideotRNAo不确定的unsureo可变区V_regionoV片段V_segmento变异variationo3'发夹区3'clipo3'非翻译区3'UTRo5'发夹区5'clipo5'非翻译区5'UTRo-10_信号-10信号_signalo-35_信号-35信号_signal•生物学来源BiologicalSourceo概述:描述符或特征Overview:DescriptororFeature?o物种页OrganismPage.名称子页NamesSubpage定位子页LocationSubpage序列位置LocationofSequence序列来源OriginofSequence遗传密码子页GeneticCodesSubpage细胞核的Nuclear线粒体的Mitochondrial谱系子页LineageSubpageo限定词子页ModifiersPage来源子页SourceSubpage物种子页OrganismSubpageo综合页MiscellaneousPage同义词子页SynonymsSubpage对照子页Cross-refsSubpage•出版物Publicationso概述:描述符或特征Overview:DescriptororFeature?o注册表中引用项CitationonEntryForm状况Status类别Class范围Scopeo引用信息表格(常规)CitationInformationForm(General)作者页AuthorsPage名称子页NamesSubpage联系子页AffiliationSubpageo引用信息表格(选择期刊作为分类)CitationInformationForm(IfselectedClasswasJournal)标题页TitlePage期刊页JournalPage备注页RemarkPage•文件菜单FileMenuo关于SequinAboutSequin5o帮助Helpo打开Openo将FASTA序列直接导入序列编辑器FASTANucleotideDirecttoSequenceEditoro关闭Closeo输出到GenBankExportGenBanko复制浏览DuplicateViewo存储Saveo另存为SaveAso恢复Restoreo准备提交PrepareSubmissiono打印Printo退出Quit•编辑菜单EditMenuo复制Copyo清除Clearo复制副本Duplicateo编辑序列Editsequenceo编辑队列Editalignmento编辑提交者信息EditSubmitterInfo提交子页Submissionpage联系子页Contactpage引用子页Citationpageo更新序列UpdateSequenceo特征传递FeaturePropagateo添加序列AddSequence•搜索菜单Searchmenuo查找ASN.1FindASN.1o查找原始文件FindFlatFileo按基因查找FindbyGeneo按蛋白质查找FindbyProteino按位置查找FindbyPositiono确认Validateo拼写检查SpellCheckoCDDBLASToORF探测器ORFFindero选择对象SelectTarget•综合菜单MiscMenuo样式管理StyleManagero网络配置NetConfigureGenBank记录更新UpdatingexistingGenBankrecords:在Medling中执行查询PerformingaMedlinelookup:执行分类学查找Perfor