中国药科大学药学专业英语课件-六味地黄丸

六味地黄丸LiuweiDihuangPillsIngredientsRadixRehmanniaePreparata160g熟地黄FructusCorni(processed)80g山茱萸（制）CortexMoutan60g牡丹皮RhizomaDioscoreae80g山药Poria60g茯苓RhizomaAlismatis60g泽泻Procedure/制法Pulverizetheabovesixingredientstofinepowder,siftandmixwell.Toeach100gofthepowderadd35～50gofrefinedhoneyandaquantityofwatertomakepillsanddry;alternativelyadd80～110gofrefinedhoneytomakesmallhoneyedpillsorbighoneyedpills.pulverize:研磨sift：评审，过筛tomakepills:泛丸DescriptionBrown-blackwater-honeyedpills,black-brownsmallhoneyedpillsorbighoneyedpills;taste,sweetandsour.IdentificationMicroscopical:Starchgranulestriangular-ovoidoroblong,24～40μmindiameter,hilumshortcleftorV-shaped.Irregularbranchedmassescolorless,dissolvedinchloralhydratesolution:hyphaecolorless,4～6μmindiameter.Parenchymagreyish-browntoblack-brown,cellsmostlyshrunken,andeachcontainingabrownnucleus-likemass.cleft：裂缝hyphae：菌丝Clustersofcalciumoxalateoccurringincolourlessparenchymatouscells,sometimesseveralclustersarrangedinrows.Epidermalcellsofpericarporange-yellow,polygonalinsurfaceview,withsomewhatbeadedanticlinalwalls.Parenchymatouscellssubrounded,withellipticalpits,gatheredintopitgroups.epidermal:表皮的pericarp:果皮pit：纹孔beadedanticlinalwalls:垂周壁念珠状增厚(2)PhysicalandChemicalidentificationTo9gofsmallhoneyedpillsorbighoneyedpills,or6gofwater-honeyedpills,inpowder,add4gofkieselguhrandtrituratewell.Heatunderrefluxwith40mlofetheratalowtemperaturefor1hour,filterandevaporatetoremoveether.kieselguhr:硅藻土triturate:研匀Dissolvetheresiduein1mlofacetoneasthetestsolution.DissolvepaeonolCRSinacetonetoproduceasolutioncontaining1mgperml,asthereferencesolution.Carryoutthemethodforthinlayerchromatography(AppendixⅥB),usingsilicagelGasthecoatingsubstanceandcyclohexane-ethylacetate(3:1)asthemobilephase.paeonol:丹皮酚cyclohexane:环己烷Applyseparatelytotheplate10μlofeachofthetwosolutions.Afterdevelopingandremovaloftheplate,dryitinair,spraywith5%solutionofferricchlorideinethanolacidifiedwithhydrochloricacidandvisualizeunderacurrentofhotair.Thebluish-brownspotinthechromatogramobtainedwiththetestsolutioncorrespondsinpositionandcolourtothespotinthechromatogramobtainedwiththereferencesolution.AssayDistil2gofthebighoneyedpills(or5gofthewater-honeyedpillsorsmallhoneyedpills),accuratelyweighed,collect450mlofdistillate,transfertoa500mlvolumetricflaskanddilutewithwatertovolume.Carryoutthemethodforspectrophotometry(AppendixⅤA),measuretheabsorbanceat274nmandcalculatethecontentofpaeonol(C9H10O3),taking862asthevalueofE1%1cm.Thebighoneyedpillscontainnotlessthan0.63%,thesmallhoneyedpillscontainnotlessthan0.07%andthewater-honeyedpillscontainnotlessthan0.10%ofpaeonol(C9H10O3),referedtoCortexMountan.distil:蒸馏volumetricflask:容量瓶spectrophotometry:分光光度法FructusCorniTo5gofpills,accuratelyweighed,add30mlofwater,allowtodisintegratethoroughlyonawarmwaterbath,add2gofthekieseguhr,stirwellandfilterthroughfilterpaper.Washtheresiduewith30mlofwater,dryat100℃andgrindintofinepowder.TransferthepowdertogetherwiththefilterpapertoaSoxhelt’sextractorandheatunderrefluxwithanappropriatequantityofetherfor4hours.disintegrate/溶解；grind/成粉末；Soxhelt’sextractor/索氏提取器Recovertheetherandmaceratetheresiduewithtwo15mlquantitiesofpetroleumether(30～60℃)forabouttwominuteseachtime.Decantthepetroleumether,dissolvetheresidueinamixtureofabsoluteethanol-chloroform(3:2)bywarming.Transferaccuratelythesolutiontoa5mlvolumetricflask,dilutetovolume,mixwell,usethesolutionasthetestsolution.macerate/浸软；decant/倒出；absoluteethanol/无水乙醇DissolveursolicacidCRS,weighedaccurately,inabsoluteethanoltoproduceasolutioncontaining0.5mgpermlasthereferencesolution.Carryoutthemethodforthinlayerchromatography(AppendixⅥB),usingsilicagelGasthecoatingsubstanceandamixtureofcyclohexane-chloroform-ethylacetate-formicacid(20:5:8:0.1)asthemobilephase.Applyseparatelytotheplate5μltestsolutionofthewater-honeyedpillsor10μlofthetestsolutionofthebigorsmallhoneyedpills,4μland8μlofthereferencesolutionalternately.ursolicacid:熊果酸formicacid:甲酸Afterdevelopingandremovaloftheplate,dryitinair,andspraywith10%solutionofsulfuricacidinethanol.Heatat105℃for5～7minutestothespotsclear.Covertheplatewithaglassplateofthesamesizeandfixupwithadhesiveplaster.Carryoutthemethodforthinlayerchromatography(thinlayerchromatographicscanningmethod.AppendixⅥB),scanatwavelength:λS=520nm,λR=700nm.Measuretheintegrationvaluesofabsorbanceofthetestsolutionandthereferencesolution,calculatethecontentofursolicacidinthepills.integrationvalues:吸光度值Thebighoneyedpillscontainnotlessthan1.17mgperpill;thesmallhoneyedpillsandthewater-honeyedpillscontainnotlessthan0.13mgand0.20mgpergramofursolicacid(C30H48O3)respectively,referedtoFrutusCorni.ActionToreplenishyinofthekidney.滋阴补肾IndicationsDeficiencyofthekidneyyimarkedbydizziness,tinnitus,achingandlimpnessoftheloinsandknees,consumptivefever,nightsweating,seminalemissionordiabetes.用于肾阴亏损，头晕耳鸣，腰膝酸软，骨蒸潮热，盗汗遗精，糖尿病。UsageandDosage6gofwater-honeyedpills,9gofsmallhoneyedpillsor1bighoneyedpill.2timesaday.用法和用量口服。水蜜丸一次6g，小蜜丸一次9g，大蜜丸一次1丸，一次2次。StoragePreserveintightlyclosedcontainers贮藏保存于密闭容器中。