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IsoleraUserManualPageiiSafetyandDocumentConventionsTheIsolerasystemshouldonlybeoperatedandmaintainedbytrainedindividuals.Pleasereadthismanualcarefullybeforeworkingwiththesystem.Toguaranteesafeandeffectiveoperationitisabsolutelynecessarytofollowalloftheinstructionsinthisusermanual.Allinformationisbelievedtobecompleteandaccurateatthetimeofpublication,butissubjecttochange.PaycloseattentiontotextwithaNOTEorWARNINGheading.ANOTEgivesinformationthatiscrucialtotheoperationbeingdescribed.AWARNINGindicatesimportantinformationthatwillpreventharmtopersonneland/ordamagetothesystem.NOTENotetextappearsinthemanualtoprovideadditionalorcrucialinformationaboutthecurrenttopic.Itmayindicateasituationinwhichthesystemmaynotperformasexpectedunlessspecificguidelinesarefollowed.WARNINGTheWarningnoteindicatesahazardthatcouldresultinseriousinjurytotheuserand/ordamagetotheequipmentunlessgoodlaboratorypractices(GLP)and/ormanufacturer'sguidelinesarefollowed.WarrantyandLiabilitySeethe“BiotageTerms&ConditionsofSale”documentat®AB:Biotage,BiotageZIP,Advancer,AdvancerKilobatch,Advancer350,AFFINILUTE,Endeavor,EVOLUTE,ExploraSep,Fireflydesign(),FLASH+,FlashMaster,FlashVac,Horizion,HPFC,HP-SIL,HP-Sphere,Initiator,Initiator+,Isolera,IsoleraFour,IsoleraLS,IsoleraOne,IsoleraPrime,IsoleraSpektra,IsoleraSpektraFour,IsoleraSpektraLS,IsoleraSpektraOne,ISOLUTE,IST,ISTdesign(),KILOPREP,KP-C18-HS,KP-C18-WP,KP-C4-WP,KP-NH,KP-Sil,MIP4SPE,MIPRuleof6,MIP[4]Process,MIP[4]Proteins,PathFinder,PRESSURE+48,PRESSURE+96,RapidTrace,RapidTrace+,Resolux,Samplet,SIM,SNAP,SNAPUltra,SP1,SP4,SPEDry96,SPEDry96Dual,SPWave,SyroWave,TurboVap,VacMaster,V-10,ZIF,ZIF-SIM,ZIP-Sphere,and1-PointSupport.Otherproductandcompanynamesmentionedhereinmaybetrademarksorregisteredtrademarksand/orservicemarksoftheirrespectiveowners,andareusedonlyforexplanationandtotheowners'benefit,withoutintenttoinfringe.CopyrightBiotageSwedenABreservestherighttomakechangestotheinformationcontainedhereinwithoutpriornotice.Nopartofthismanualmaybereproducedortransmittedinanyformorbyanymeans,electronicormechanical,foranypurpose,withouttheexpressedwrittenpermissionofBiotageSwedenAB.©Copyright2007–2012BiotageSwedenAB.Allrightsreserved.UserDocumentationListThefollowingdocumentsareprovidedonthe“IsoleraUserDocumentation”CD(P/N411831):•“IsoleraInstallationandSafety”,P/N411828•“IsoleraUserManual”(thispublication),P/N411829•“QuickGuideforIsolera”,P/N411830•“IsoleraSafetyTranslations”,P/N412861TableofContentsPageiii1:DescriptionandSpecifications-----------------------------------1-1GeneralSystemDescription--------------------------1-1BasicSystemComponents-------------------------1-2SystemConfigurations---------------------------1-2ProgrammedFunctions---------------------------1-3UserDocumentation----------------------------1-3Accessories--------------------------------1-3SoftwareDescription------------------------------1-7MainMenu--------------------------------1-7DataAdministrationMode-------------------------1-7SystemMode-------------------------------1-8ChemistryMode------------------------------1-9MethodTab(ChemistryMode)------------------------1-11StatusTab(ChemistryMode)------------------------1-11ResultsTab(ChemistryMode)------------------------1-14SolventsTab(ChemistryMode)-----------------------1-15IsoleraRemoteViewer---------------------------1-16Specifications---------------------------------1-17GeneralSystemSpecifications------------------------1-17OperationalPerformanceSpecifications--------------------1-18SolventSpecifications---------------------------1-19ChemicalResistanceofthePeristalticPumpTube(IsoleraLS)----------1-202:DataAdministration------------------------------------------2-1LogintotheDataAdministrationMode----------------------2-1AdministratetheCartridgeList-------------------------2-2AddaCartridgeType---------------------------2-2DeleteanUnusedCartridgeType----------------------2-2EditaCartridgeType----------------------------2-3SelectCartridgeTypestoList------------------------2-3AdministratetheMethodList--------------------------2-3DeleteMethods------------------------------2-3ExportMethods------------------------------2-4ImportMethods------------------------------2-4SetDefaultMethods----------------------------2-4AdministratetheRackList---------------------------2-5AddaRackType-----------------------------2-5DeleteanUnusedRackType------------------------2-6EditaRackType-----------------------------2-6SelectRackTypestoList--------------------------2-6AdministratetheResultList---------------------------2-6ExportandDeleteResultRecords----------------------2-7CleanUptheSystem’sDatabase-----------------------2-7PageivAdministratetheSolventList--------------------------2-8AddaSolvent-------------------------------2-8DeleteanUnusedSolvent-------------------------2-8EditaSolvent-------------------------------2-9SelectSolventstoList---------------------------2-9AdministratetheUserList---------------------------2-9AddaUserA