三种重要木质素降解酶研究进展

:2003-02-09:(1978-),,;(1942-),,,,::(001200217):1008-9632(2003)05-0009-03,(河南师范大学生命科学学院,新乡453002):就三种重要木质素降解酶:LiPMnP和漆酶在自然界的分布,化学组成结构特征降解机制分子生物学等进行综述,并探讨了其作用协同性:木质素过氧化物酶;锰过氧化物酶;漆酶;:Q936:ACOD,-,C-C,1934BoruffBuswell,,19831984(LiP)(MnP),(Laccase),,1(LiP)11LiP(Phanerochaetechrysosporium),LiP,LiP,11LiPTrametesT.gibbosa,T.versicolorPhlebiaP.brevispora,P.radiata,P.ochraceofulva,P.tremellosa,P.adustaCoriolusC.consors,C.hirsutusOthersBjerkanderaadusta,Chrysoniliasitophila,Chrysosporiumpruinosum,Coriolopsisoccidentalis,Phellinuspini,Polyporusostreiformis,Phanerochaetechrysosporium,Streptomycesviridosporu,Pleurotusostreatus,Junghuhuniaseparabalima,Fomeslignosus12LiPFe3+(IX),LiP,,(heme),VA,,LiP,LiPFe3+,H2O2LiP(+Fe4+),LiPLiP!(Fe4+),,,His82,Trp170,:-His82-Ala83-Asn84-His47-Heme-Trp170-Leu171-Heme[1]LiP,,,,,H2O2,:LiP+H2O2∀LiP+H2OLiP+SH2∀SH+LiP!LiP!+SH2∀SH+LiP2SH+2∀2+2SH2,SH2H2O2H2O2,,13-O-4,-O-4A,B,LiPLiP-O-4C-CVA2-C-,:C-C-9205200310JOURNALOFBIOLOGYVol.20No.5Oct,2003,,;C-C-,,VALiP,LiPH2O2VALiP,H2OCO2,LiP,,/,,,Kerb,,CO2,,,LiP,-1,LiP-5-O-4,14,P.chrysosporiumTrametesversicolorBjerkanderaadustaP.chrysosporium7LiP,LiPA-LiPJ,,10,LiP[2]LiP,53%~98%,LiP343~345,36360~36607DaN21,67LiP89,49~78bp,5#TATA(-66~-81bp)CAAT(-107~-228bp),C,N,LiP,:H1H2H6H8H10,NN,H8,cDNAML-1,H2,H6N,LiP,LiP,,Mn2+[3]2(MnP)21MnPLiP,,,MnP2,,,,22MnP17%,Mn2+,MnP,H2O2,Mn3+Mn3+MnP,,,,MnO2,MnO2LiPH2O2LiP-MnP,Mn2+Mn3+,,LiP,MnP,MnLiPMnP,MnP,MnPH2O2,Mn2+Mn3+,,Mn3+LiP2MnPPleurotusP.sapidus,P.erynigii,P.pulmonariusP.sajor_cajuPhlebiaP.brevispora,P.radiataPanusP.tigrinus,P.conchatusGanodermaG.lucidum,G.valesiacumTrametesT.gibbosa,T.versicolor,T.villosa,T.cingulataOthersCeriporiopsissubvermispora,Coriolopsispolyzona,Lentinulaedodes,Daedaleopsisconfragosa,Dichomitussqualens,Pycroporuscinnabarinus,Grifolafrondosa,Rigidoporuslignosus,Phanerochaetechrysosporium,stereumhirsutum,cyathusstercoreus,Perenniporiamedulla_panis23MnP,1,AH,1MnP24P.chrysosporiumMnP,RNAPCR,MnPmRNA:MnP1MnP2MnP3,MnP2MnP,,MnP1,MnP3MnP67,N2124,,MnP51TATAA(-81bp)3CCAAT,[4~5]10205200310JOURNALOFBIOLOGYVol.20No.5Oct,2003,MnPMn2+CN,P.chrysosporiumOGC101MnP1357,6,57~72bp3311883,,,(Basidimycetes)(Polyporus)(Podospora),,,[6],(Azospirillumlipoferum)[7]3264~390kD,Podosporaanserina,,500,:Cu2+,,,,614nm;!Cu2+,,,,,;∃Cu24+,,,(Cu2+-Cu2+),330nm,,,250,,,,33O2,,,,,-,,,,,,Laccase/O2O2,,O2,:Cu2+Cu2+Cu24+2eCu+Cu+Cu24+Cu2+Cu2+Cu22+2eCu+Cu+Cu2+2H+O2H2OCu2+Cu2+(Cu2O)3+2H+H2OCu2+Cu2+Cu24+34,,AgaricusbisporusNeurosporacrassaCoprinuscinerens3Lcc1Lcc2Lcc3Lcc17,54~70bp,521,N-,C23,ArgLys,CLcc2Lcc313,80%Lcc3Lcc158%,Lcc2Lcc159%,Lcc3Aspergillusnidulan18%,,[8~9],,A.oryazeTATAamylasePichiapasti,Lcc1,90%[10],,4,,,CNpHP.cinnabarinus[11],,LaccaseMnP,,,,,,Mn2+LiP,MnPLaccase,,:LiP-MnP,P.chrysosporium;LiP_MnP_Laccase,Trametesgibbosa;MnP_Laccase,Lentinulaedodes;LiP_Laccase,Pleurotusostreatus,[2],,:[1]SusanC,etal.DescriptionofaversatileperoxidaseinvolvedinthenaturaldegradationofligninthathasbothManganeseperoxidaseandligninperoxidasesubstrateinteractionsites[J].BiolChem,1999,274(15):10324~10330.[2]ReddyCA.AnoverviewoftherecentadvancesonthephysiologyandmolecularbiologyofligninperoxidaseofP.chrysosporium[J].Biotechnol,1993,30(1):91~107.[3]DosoretzCD,etal.OverproductionofligninperoxidasebyP.(33)11205200310JOURNALOFBIOLOGYVol.20No.5Oct,2003[4]唐子永,王延玲,程淑云.人参果茎尖脱毒及试管快繁技术[J].内蒙古农业科技,1992(12),18.[5]蔡润,等.人参果茎尖培养[J].上海农学院学报,1997,17(3).233~236.[6]傅玉兰,李春生,许清松.人参果的组培快繁[J].安徽农业大学学报,2002,29(2),132~136.[7]朱广廉.植物组织培养中灭菌和无菌操作的几个问题[J].植物生理学通讯,1995,31(5),373~375.[8]陈利萍,陈竹君,董伟敏.日本茄F1离体快速繁殖[J].植物生理学通讯,1996,32(1),26.[9]王首锋,梁海曼.升汞和次氯酸钠对黄瓜种子萌发及幼苗生长的影响[J].植物生理学通讯,1996,32(2),117~120.[10]潘瑞炽,董愚得.植物生理学[M].北京:高等教育出版社,2000年,190.SheddingvirusandrapidpropagationofSolanummuricatumAit.LANWei1,LIANGjun2(1.Departmentofbiology,FuyangNormalCollege,Fuyang,236032,China;2.Departmentofbiology,AnhuiEducationcollege,Hefei,230061,China)Abstract:AsepticsatusstemapexoftheSolamummuricatumAitwerecultured.Thesheddingvirusseedlingscouldbeobtainedafterfivegenerationsofhigh_temperaturetreatedplusstemapexdelamination.TheasepticseedlingswereculturedinMS+6-BA1.0mg/L+NAA0.1mg/L+2,4_D0.10mg/Lmedia.Thelightgreen-yellowcallicoulddifferentiateintobuds.AftertransferingcalliwithbudstoMS+6-BA1.0mg/L+IAA0.05mg/Lculturalmedia,thecallidifferentiateintorosettebudswhichcouldproliferatebetter.Theclusterofbudsgrewrootseasilyin1/2MS+NAA0.1mg/L+PP3330.2mg/Lculturalmedia.AtlastthetubeplantsofSolanummuricatumAit.couldbeobtainedKeywords:SolanummuricatumAit.;Sheddingvirus;rapidpropagation(11)[1]chrysosporium(BKM_F_1767)undernutrientcondition[J].ApplEnviroMicrobiol,1999,65(11):4943~4948.[4]GettemyJM,etal.Reversetranscription_PCRanalysisoftheregulationofthemanganeseperoxidasegenefamily.ApplEnvirMicrobiol,1998,64(2):569~574.[5]GoldMH,AlicM.Molecularbiologyofthelignin_degradingbasidiomycetePhanerochaetechrysosporium[J].MicrobiolRev,1993,57(3):605~622.[6]张甲耀,龚利萍,罗宇煊,等.嗜碱细菌复合碳源条件下对麦草木质素的降解[J].环境科学,2002,23(1):70~73.[7]王佳玲,余惠生,付时雨,等.白腐菌漆酶的研究进展[J].微生物学通报,1998,25(5):233~236.[8]CullenD.Recentadvancesonthemoleculargeneticfungi[J].Biotechnol.,1