干化学法_电极法与湿化学法测定血糖抗干扰实验对比

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:R446.11+2:A,,(,310009):(Glu)Vitros250BECK2MANSynchronCX3OlypumsAU22700CC7.8mg/dlHb250mg/dl2.00mg/dl;400mg/dl8.00mg/dl;Hb1000mg/dl,;,;,,:;;;(Glu),,[1]Glu,Glu,11.11.1.1Vitros250BECKMANSynchronCX3OlypumsAU227001.1.2(1)(GOD)Glu(0019-0615-5195)(0191);:BECKMANGlu(M202080)(0302202);(HK):Glu(DG925)(104);(2)BECKMAN(M807072);(970302);(20020612)1.1.3(1)C(500mg/dl):,0209202;(2)(140g/L):EDTA,4,,;(3):(),990505(4)(16.7g/L):Intralipid(30%),020306011.1.4:100,2ml,,1000r/min,30,150ml1.21.2.1Vitros250CX3OlypumsAU22700(BECK2MAN),,1.2.21.2.2.1C1.2.2.20.5mol/LNaHCO3=235[2],OlypumsAU2270064mg/dl1.2.2.311,6(Vcmg/dl):3.97.815.631.362.5125.0;Hb(mg/dl):12.50250.0500.01000.02000.04000.0;Bil(mg/dl):1.02.04.08.016.032.0;TG(mg/dl):50.0100.0200.0400.0800.01600.0)1.2.2.4:(/)111.2.2.5Vit2ros250CX3OlypumsAu22700,5,1.3SPSS22.1111200421ZhejiangJournalofLaboratoryMedicine,2004,Vol.2,No.11(n=20)€xs(mmol/L)6.040.056.060.076.100.07CV(%)0.881.121.062.2Glu,Glu,:2.2.1CVc7.8mg/dl,,;2.2.2(Hb)Hb250mg/dl,Hb;Hb1000mg/dl,Hb;2.2.3(Bil)Bil2.00mg/dl,Bil;Bil8.00mg/dl;2.2.4(TG)TG400.0mg/dl,TG;13(,GluGlu)33.11.0034.69mmol/L,0.0025.00mmol/L,0.0025.00mmol/L(),,,CV[3],,3.2,,(GOD),H2O2;C,(,,,,,),,[4][5],(),3VC62.5mg/dl125.0mg/dlGlu,1()2()3()TG400.0mg/dl,[4][6],:(HK),GOD,NADPH,;21200421ZhejiangJournalofLaboratoryMedicine,2004,Vol.2,No.1Glu,GluO2H2O2,GluO2,O2Glu,H2O2,I-H2O2,H2O2,VcBilTG,Hb1000mg/dl3.3(TurnaroundtimeTAT),10,15,3085%7%BSA,,;,;,,,,:[1],.[J].,1985,5:1.[2],,[J].,2001,2:102-103.[3],.[M].:,1996,616-633.[4],,,.[J].,2000,18:138-139.[5]HernryC.RoydenNR.EarlyHistoryofEastmanKodakEktachemslides[J].ClinChem,1997,9:1647.[6],,,:[J].,2002,4:210-211.Comparedtheanti2interfereabilityamongthebloodglucosemeasurementusingdry2chemistry,ISEandEnd2PointchemistrySHENLing2wei,HEXiao2hui,QINGuang2ming(TheSecondAffiliatedHospital,SchoolofMedicine,ZhejiangUniversity,Hangzhou310009,China)Abstract:ObjectiveToinvestigatetheanti2interfereabilityamongglucosemeasurementusingdry2chemistry,Ion2Selective2Electrode(ISE)andEnd2pointchemistry.MethodsUsingdry2chemistry,ISEandEnd2ponitchemistrytodetermineandcomparethelinearity,precisionandanti2interfereabilityofbloodglucosemeasurement.ResultsThelinearityare1.034.69mmol/L,025mmol/Land025mmol/L,respectively.Thereisnointerferewhentheconcentrationofvitamincis7.8mg/dl,Hbis250mg/dlandbilirubinis2.0mg/dlindry2chem2istry,thereisnointerferewhentheconcentrationofTGis400mg/dl,andbilirubinis8.0mg/dlinEnd2pointchemistry,thereisnointerferewhenHbconcentrationisupto1000mg/dlinISE.ConclusionISEisarapid,stableandstronganti2interferemethodtosuitforemergencybloodglucosetest.End2pointchemistryissuitabletoroutinebatchbloodglucosemeasurement.Dry2chemistryisasimpleandflexiblemethodtosuitforemergencybloodglucosetest,butissanti2interfereabilityisnotgoodenough.Keywords:dry2chemistry;ion2selective2electrode;end2pointchemistry;bloodglucose(:2003210212,:2003212220)(:)(8)3.7.1a)b)c)d)75%,3.7.23.7.3a):,b):80mm/Hg,c)d)e)f)3.7.4a)b)c)(:)31200421ZhejiangJournalofLaboratoryMedicine,2004,Vol.2,No.1

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