搜索
(,400715),,,:2007-11-19:(CSTC2007BB5370):(1984),,;(1964),,,:ExtractionandAnalyticalmethodofPolysaccharidesLiuTingZhouGuangming3(SchoolofChemistryandChemicalEngineering,SouthwestUniversity,Chongqing400715)AbstractThenewestextractionandanalyticalmethodofpolysaccharidesinrecentyearswasintroduced,andsomeanalysisanddeterminationtechnologyforpolysaccharideswerereviewedaccordingtotheclassificationofthedifferentpartsofanalysis1Moreover,accordingtoclassificationofthedifferentaspectsofmethodandoperationsofdeterminationtechnologythecomparisonoftheadvantagesanddisadvantageswerediscussed1Andthereferencesforthefurtherdevelopmentsandre2searchmentsofpolysaccharidesanalysiswereprovided1The44referenceswereadopted1Keywordspolysaccharidesextractionanalyticalmethod,(polysac2charides,PS),,,,2060,[19],,,,;,,,,,,,,1,,[10],,,[9],[11],,,[12],,500g,,,115%50pH41860min,10L,95,,952h,,,,,[13],,100g50,3,5min,,;95%,15min,5,-50,,6622320083ChemicalIndustryTimesVol.22,No.3Mar.3.2008[14],70,600W,30min,,2211,,-[15]-[16],,,,,-,-[17]-,0185%0101%,1141%0106%,2163%0127%,[18],,,,3%315%4117%6135%3110%3153%,,,,-[19],,,,,,,-,3,5-(DNS),,,,[20],212(HPLC),(GC),(TLC),(HPCE)[21]1--3-5-(PMP),9,,,,,,,,,7,01571100011331068116711712169,,,[14],MS:VF-5MS(30m0125mm,Varian);280;120,3/min210,4min;,110mL/min;EI-Auto70eV;180;280;40550amu-[22],,,,(=8212),-[23],,-,:75mmol/L(pH=915);22;16kV;200nm;,5s,3,,[24],[25],HPLC,,,,,(R1)(ELSD)RI,ELSD,HPLC,7620081Vol122,No.3,GC,,,,,,,(TLC),,,,,,HPCE,,,,,,,Roxklin[2635]1983,(HPAEC)0101012mol/LNaOHNaAcpHpKa[2728],[2933]Cai[34]-,16min,,Tom2maso[35],,,,,,213,,:[36]:,10000r/min20min,,,,,270280cm:,(12601210cm-1)(800950cm-1)(40006500cm-1),[37]TPS-334153280cm-116651635cm-1:13CNMR,1HNMR[38]FT-IR13CNMR,,[39],,,,,[40],,,,[41],[42][43],,;,,;,[14],,90min,300W,1mol/L,3,,,,,,,,,,8620081Vol122,No.3OverviewofThesises[1]1[J]1,2007,(5):5051[2],,,1[J]1,2007,32(12):12451247[3],,,1[J]1,2007,42(8):630632[4]TanFeng,DengJun1AnalysisoftheconstituentsandantisenilefunctionofAchyranthesbidentatapolysaccharides[J]1ActaB2otanicaSinica,2002,44(7):795798[5],,,,1[J]1,2007,16(1):1116[6]1[J]1,2005,22(6):703707[7],1[J]1,2007,24(2):113114[8],,,1[J]12007,28(2):305308[9],,,,1[J]1,2007,28(6):153155[10],,,1Box-Behnken[J]1,2007,30(5):591594[11],,,1[J]1,2006,22(11):213216[12],,,1,[J]1,2007,28(4):217219[13],,,,1[J]1,2007,30(4):475477[14],,1-[J]1,2006,34(12):16971701[15]1-16[J]1,2005,314316[16],1-[J]1,2005,26(7):185188[17],,1[J]1,1995,26(5):255257[18],,1[J]1,2001,(11):6973[19],1[J]1,2007,(3):5861[20],,,,13,5-[J]1,2005,36(5):628630[21],,,,,1[J]1,2005,33(9):12871290[22],,,,1TLC[J]1,2006,27(12):603607[23],,,,1[J]1,2005,36(8):11641166[24],,,,1[J]1,1999,27(2):245[25],,1[J]1,2006,34(11):1665[26]RendlemanJA1Ionizationofcarbohydratesinthepresenceofmetalhydroxidesandoxides,carbhydratesinsolution[J]1Ad2vancesinChemistrySeries1WashingtonDC:AmericanChem2icalSociety,1973,117:51[27],,,1[J]1,1993,14(7):914917[28],1[J]1,1995,13(5):320324[29]LouisetteJ,BasaandMichaelW1Analysisofglycoprotein-deriveroligosaccharidesbyhigh-pHanion-exchangechro2matography[J]1JournalofChromatographyA,1990,499:205202[30]CarboPacPA10andCarboPacMA1product1Manual1Sunny2vale1CA1American:DionexCoporation,2004,4:15[31]PeterHermentin,ReinhildWitzel,JohannesFG,vliegenthaxt,JohannisPKamerling,ManffedNimtzandHaraldSConradt1AstrategyforthemappingofN-glycansbyhigh-pHanion-exchangechromatographywithpulsedamperometricdetec2tion[J]1AnalyticalBiochemistry11992,203:281289[32]KoykoKoizumi,YokoKubota,HisakoOzaki,ShigenobuKeiko,MasakoFukudaandToshikoTanimoto1Analysesofi2somericmono-O-methyl-D-glucoses,D-glucobioses9620081Vol122,No.3andD-glucosemonophosphatesbyhighperformanceanion-exchangechromatographywithpulsedamperometricdetection[J]1JournalofChroltmtographyA,1992,595:340345[33],,1[M]11:,2005;7682[34]YaqiCai,JingshenLiu,YaliShi,LinaLiangandShifenMou1Determinationofseveralsugarsinserumbyhigh-per2formanceanion-exchangechromatographywithpulsedam2perometricdetection[J]1JoumalofChromatographyA12005,1085(1):98103[35]TommasoRICataldi,MassimilianoAngelotti,GiulianaBian2co1Determinationofmono-anddisaccharidesinmilkandmilkproductsbyhigh-performanceanion-exchangechro2matographywithpulsedamperometricdetection[J]1AnalyticaChimicaActa12003,485:4349[36],[J]1,2001,15(4):6061[37],1[J]1,1995,(5):1819[38],,,1[J]1,2005,(12):911117[39],,,1[J]1,2005,18(4):692695[40]1[M]1:,1987,77120[41]JDeanPakulski,RonaldBenner1AnimprovedmethodforthehydrdysisandMBTHanalysisofdissolvedandparticulatecar2bohydratesinseawater[J]1MarineChemistry,1992,40:143160[42]SverreMMyklestad,EiinSkanoy,SolveigHestmann1Asensi2tiveandrapidmethodforanalysisofdissolvedmono-andpolysaecharidesinseawater[J]1MarineChemistry,1997,(56):279286[43]DeRuiterGA,ScholsHA1Carbohydrateanalysisofwater-solubleuronicacid-containingpolysaccharideswithhigh-performanceanion-exchangechromatographyusingmethanolysiscombinedwithTFAhydrolysisissuperiortofourothermethods[J]1AnalyticalBiochemistry,1992,207(1):176185[44],1[J]1,2005,25(10):12851288,30,,,,2007,,,,,80%Chempolis,,,,,20074,,Chempolis()0720081Vol122,No.3Overvi