常见载体引物与序列

PrimerofVectorVectorPrimer(F)Primer(R)pACTT7T3pACT2GAL4ADpACT2-RpAS2-1GAL4BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/M13-M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA1301(1300)P1P2pCAMBIA2300M13R(-48)M13F(-47)PCANTB5ES1/M13RS6pCAT3-enhancerRVP3此载体无反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025在T7前面）BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCIT7（17Base）此载体无反向引物pCI-neoT7（17Base）T3pCMS-EGFPT7T3pCMV-3Tag-4AT3/PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpCMV5FpCMV5RpCMV-MYC/HApCMV-FpCMV-RpCMV-SportM13F/T7SP6/M13RpCMV-Tag(KAN+)T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T7BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N-3’(距离很近,一般不用)pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3’pDSRED-N1(KAN+)pEGFP-N-5’PDSRED-N-RpECFP-CpEGFP-C-5’pECFP-C-3'pECFP-N1pEGFP-N-5’pEGFP-N-3’pEF/myc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5’pEGFP-C-3'pEGFP-N(KAN+)pEGFP-N-5’pEGFP-N-3’pENTR/D-TOPO(KAN+)M13FM13RpET-*(His)(KAN+)T7T7TerpET22(a,b,c)(AMP+)T7T7TerpET28,30,24,49(KAN+)T7T7TerpET32(a,b,c)(AMP+)T7/S.tagT7TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7TerInvitrogen中国使用的测序载体和引物pEYFP-N1pEGFP-N-5’pEGFP-N-3’pEYFP-C1pEGFP-C-5’pEGFP-C-3'pFLAG-CMVpFLAG-CMV-FpFLAG-CMV-RpGAD424GAL4AD3'ADpGADGHGAL4AD3'ADpGADT7-RecGAL4AD/T73'ADpGAPZa-Aa-FACTOR3'AOXpGBKT7(Kana+)T73'BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5’pGEX-4T-3’pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)PinpointTMVectorPinpointPrimerSP6pIRESPIRES-F/T7T3/PIRES-R(J40720)pIRES2-EGFPpIRES2-EGFP.P5’pIRES2-EGFP.P3’pLXSNpLXSN-FpLXSN-RpMAL-c2EMalEprimerM13F(-47)pMAL-C2xP5’P3’pMAL-p2XMalEprimerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+、ZEO+）5’AOX/a-factor3’AOXpPICZa5'AOX/PPICZa-F3'AOXpPROEXHTAM13R(-48)无反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSilencer3.1-H1hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSport1SP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(优先使用M13F）T3/M13RpT-AdvM13F/T7M13RpTARGETTMpTarget.F(在T7前面）/T7pTarget.RpTO-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25'pTriPLEx2T7pTWIN-1T7T7TER(客户确认再用）pUC18(19)/118(119)M13F(-47)M13R(-48)pUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)pXT7T7SP6pYES2T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVERCMV-F此载体无反向引物pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSP64/65SP6此载体无反向引物pSTBlue-1T7SP6pT7Blue(R)T7M13F(-47)引物名称序列(5'-3')M13RCAGGAAACAGCTATGACCM13FTGTAAAACGACGGCCAGTM13F(-47)CGCCAGGGTTTTCCCAGTCACGACM13R(-48)AGCGGATAACAATTTCACACAGGAM13(-96)CCCTCATAGTTAGCGTAACGSP6ATTTAGGTGACACTATAGT7TAATACGACTCACTATAGGGT7terminatorTGCTAGTTATTGCTCAGCGGT3ATTAACCCTCACTAAAGGGApGEX-4T-5'GGGCTGGCAAGCCACGTTTGGTGpGEX-4T-3'CCGGGAGCTGCATGTGTCAGAGGGLp1TGTATCTTATGGTACTGTAACTGGLp2CTTTATGTTTTTGGCGTCTTCCARVp3CTAGCAAAATAGGCTGTCCCRVp4GACGATAGTCATGCCCCGCGpcDNA3.1RTAGAAGGCACAGTCGAGGPinPointprimerCGTGACGCGGTGCAGGGCGpCMV-FTCTAAAAGCTGCGGAATTGTpCMV-RTCCAAACTCATCAATGTATCpTRC99C-F:TTGCGCCGACATCATAACpTRC99C-R:CTGCGTTCTGATTTAATCTGpCEP-F:AGAGCTCGTTTAGTGAACCGEBV-R:GTGGTTTGTCCAAACTCATCpIRES2-EGFP.P5’:GTAGGCGTGTACGGTGGGAGpIRES2-EGFP.P3’:AACGCACACCGGCCTTATTC3'AD:AGATGGTGCACGATGCACAGCMV-FCGCAAATGGGCGGTAGGCGTGS1CAACGTGAAAAAATTATTATTCGCS6GTAAATGAATTTTCTGTAGTAGG5`AOX1GACTGGTTCCAATTGACAAGC3`AOX1GCAAATGGCATTCTGACATCCα-FactorTACTATTGCCAGCATTGCTGCGAL4ADTACCACTACAATGGATGpACT2-RGTGCACGATGCACAGTTGAApB42ADF:CCAGCCTCTTGCTGAGTGGAGATGpB42ADR:AAGCCGACAACCTTGATTGGAGpEGFP-N-5’:TGGGAGGTCTATATAAGCAGAGpEGFP-N-3’:CGTCGCCGTCCAGCTCGACCAGpEGFP-C-5’:CATGGTCCTGCTGGAGTTCGTGpEGFP-C-3':TATGGCTGATTATGATCAGTPBV220F:AAGAAGGGCAGCATTCAAAGPBV220R:CTGCGTTCTGATTTAATCTGU6ATGGACTATCATATGCTTACCGTA2.0revprimer:AGGCGATTAAGTTGGGTA3.0rev:GAGTTAGCTCACTCATTAGGCS.tagGAACGCCAGCACATGGAC5'TriplEx2CTCCGAGATCTGGACGAGCRVP4GACGATAGTCATGCCCCGCGRVP3CTAGCAAAATAGGCTGTCCCpQE30-RGTTCTGAGGTCATTACTGGpQE30-FTGAGCGGATAACAATTTCACInvitrogen中国测序通用引物序列pEF-FTCAAGCCTCAGACAGTGGTTCpDSRED-N1-RTGAAGCGCATGAACTCCTTGpDsRED-express-C1-FTCCCACAACGAGGACTACACpCMV5RATTATAGAGGACACCTAGTCpCMV5FTTCCAAAATGTCGTAATAACP5'TGCGTACTGCGGTGATCAACP3'CTGCAAGGCGATTAAGTTGGBAC2ACGCACAGAATCTAGCGCTTBAC1AACCATCTCGCAAATAAATA3'BD:TAAGAGTCACTTTAAAATTTGTATACpTarget.FCCAGGATTTTCCCAGTCACpTarget.RGGCTTTACACTTTATGCTTCT7(17base)ACATCCACTTTGCCTTTCTCpYes2.RTCGGTTAGAGCGGATGTGGAL4BDTCATCGGAAGAGAGTAGpAS2-1.RAAGAGTTACTCAAGAACAAGAApFlag-CMV-F:GGTAGGCGTGTACGGTGGpFlag-CMV-R:GCACTGGAGTGGCAACTTpIRES-F:CTTACTGACATCCACTTTGCpIRES.R:CACTGCATTCTAGTTGTGGTP1CCAGGCTTTACACTTTATGCP2GCGATTAAGTTGGGTAACGCpLEXA-FCGTCAGCAGAGCTTCACCATpLEXA-RTAAAACCTAAGAGTCACTTTpLNCX-FAGCTCGTTTAGTGAACCGTCAGATCGpLNCX-RACCTACAGGTGGGGTCTTTCATTCCCpLXSN-FCTTGAACCTCCTCGTTCGACpLXSN-RGTTGCTGACTAATTGAGATGpSHUTTLE-CMV-FGGTCTATATAAGCAGAGGTGpSHUTTLE-CMV-RGTGGTATGGCTGATTATGATCAGMalEPrimerGGTCGTCAGACTGTCGATGAAGCC