规模化沼气工程沼液中微生物的细菌种群分析与功能初探

2012-10-302012-10-31202－37“”2011BAD11B05MF201005182010BAD03B01。[e-mail]陈超1，徐凤花1，高立洪2，吴进3，韦秀丽2，宋金龙4，王彦伟4，阮志勇41．1500302．4013293．6100414．100081NPKBLAST32。pH6．1～8．715．4%0．94。105～106CFU·mL－1。Paenibacilluspeoriae、、、。S216．4A1000－1166201206－0007－05PopulationStructureAnalysisandFunctionExplorationoftheBacteriainBiogasSlurryfromLarge-scaleAnaero-bicDigestionProjects/CHENChao1XUFeng-hua1GAOLi-hong2WUJing3WEIXiu-li2SONGJing-long4WANGYan-wei4RUANZhi-yong4/1．ResourcesandEnvironmentalCollegeNortheastAgriculturalUniversityHarbin150030China2．ChongQingAcademyofAgriculturalSciencesChongqing401329China3．BiogasIn-stituteofMinistryofAgricultureChengdu610041China4．KeylabortoryofMicrobialResourcesMinistryofAgricultrueInstituteofAgriculturalResourcesandRegionalPlanningChineseAcademyofAgriculturalSciencesBeijing100081ChinaAbstractBiogasslurrysampleswerecollectedfrom10large-scalebiogasprojectwhichoperatedbyModernFarmingGroupsCo．Ltdindifferentregion．Thespeciescompositionandmicrobequantitiesweredeterminedwhilethedetermina-tionofbiogasslurrypropertieswerecarriedout．Thirtytwostrainswereisolatedfromthesesamplesbyusingtraditionalcul-turemethod．Theresultsshowthatthebacteriainbiogasslurrywerebetween105～106CFU·mL－1．ThoughthegroupofbacterialcommunityindifferentsampleswerediversityBacilluswerethemaingroupinallsamples．KeywordsLarge-scaleBiogasProjectbiogasslurrybacteriapopulationsAntagonistic12011385073032。、。17ChinaBiogas2012306、、、2～3。。5～67。8910。。22．110。Fusariumoxypori-umf．sp．vasinfectum、Fusdrzumoxysporiumf．sp．cucumerinum、RhizoctoniacerealisVanderHoeven、Alternariaalternata。2．22．2．1pHpHPHS-2F-TOCElementarliquiTocIIGermanytotalnitrogenTNtotalphosphorusTPdissolvedphosphorusDPtotalpotassiumTKFIAstarTM5000SystemsFOSSAmeri-ca。2．2．2YPPCALBMRSPCAPCA10－510－610－7PCA30℃48。。16SrDNAPCR11～1216SEU27F5'-AGAGTTTGATCCTGGCTCAG-3'1492R5'-GGTTACCTTGTTACGACTT-3'PCR25μL0．5μLDNA1μLPCRMIXFermen-tas13μLddH2O10μL95℃5min94℃1min55℃1min72℃1min30s3072℃10min12。PCR1%。EzTaxonserver2．113。2．2．39PDA1cm0．5cm28℃3～5d2ClustalxMEGA5Neigh-borJoining15boot-strap1000。33．110pH110pH6．1～8．7。15．4%、、4．27%1．62%9．52%。0．94%、、0．43%0．2%0．31%。100%。8ChinaBiogas20123068900MPN。。1/%0．941．721．1111．451．3710．21．770．9815．4/%64．674．486．36．345．0416．242．763．9464/%6294．895．19394．394．983．594．594．491pH7．68．78．46．18．58．58．68．17．668．6/%100100100100100100100100100100/MPN·mL－1＜3210930．043．62389009．2460＜30/%0．430．490．340．460．440．382．650．730．354．27/%0．20．890．640．20．690．751．640．170．161．62/%0．310．340．130．320．320．245．950．810．259．523．21．2PCA30℃48h1105～106CFU·mL－1。3．9×105CFU·mL－11．87×106CFU·mL－1。116SrDNABLAST26743Bacillussp．64%。1617。2-7Ba-cillusmethylotrophicusCBMB2052-4-3Corynebacteri-umefficiensYS-3147-4LysinbacillusmacroidesLMG18474BacillusamyloliquefaciensStreptomycescellulosaeNBRC13027BacillusmethylotrophicusCBMB205。。EnterobacterRhodococcus。3．336732。3281-42-74-67-b38-29-910-510-84-6Paenibacilluspeoriae。82。41pH6．1～8．715．4%0.94%。9ChinaBiogas2012306211-41-13-24-29-41-31-4BacillusmojavensisIFO1571899．9%AB021191210-41-54-57-17-38-39-810-411-112-2Bacillusaltitudinis41KF2b99．6%AJ83184232-17-b3BacillusatrophaeusJCM907099．8%AB02118142-22-22-34-42-4-18-29-7Bacillusstratosphericus41KF2a100．0%AJ83184152-6BacilluscirculansATCC451399．9%AY72469062-7BacillusmethylotrophicusCBMB20599．7%EU19489773-33-43-5-24-17-27-59-510-312-312-4BacillussafensisFO-036b99．9%AF23485489-133-79-133-8BacillusanthracisATCC1457899．8%AB19021798-610-510-8Bacillussubtilissubsp．inaquosorumBGSC3A2899．9%EU138467109-2BacillusoleroniusDSM935699．4%X824921110-2BacillusfordiiR-719099．4%AY443039129-9Bacillusamyloliquefaciens99．4%131-6PaenibacilluscookiiLMG1841999．2%AJ250317144-6PaenibacilluspeoriaeDSM832099．5%AJ320494158-1-2PeanibacilluslactisMB187199．2%AY257868163-69-1-110-6SolibacillussilvestrisHR3-2399．1%AJ006086177-48-712-5LysinbacillusmacroidesLMG1847499．6%AJ628749183-5-19-6-112-6-2PusillimonasnoertemanniiBN997．5%AY695828192-4-2BrevibacilluscentrosporusDSM844598．8%AB112719201-2ProvidenciavermicolaOP199．9%AM0404952112-6-1PseudomonasindoloxydansIPL-197．4%DQ916277222-4-3CorynebacteriumefficiensYS-31495．8%BA000035233-1EnterobacterhormaecheiATCC4916299．1%AFHR01000079248-1-1RhodococcusequiDSM2030798．5%X80614258-8Alcaligenesfaecalissubsp．faecalisIAM1236999．3%D88008269-1-2Klebsiellapneumoniasubsp．ozaenaeATCC1129699．8%Y17654279-3ArthrobactergandavensisR581299．2%AJ316140289-6-2StreptomycescellulosaeNBRC1302798．9%AB184265299-11SporosarcinaluteolaY199．6%AB473560309-12AcinetobactertowneriAB111098．4%AF5098232105～106CFU·mL－1BacillusmojavensisBacillusaltitudinisBacillusatro-phaeusBacillusstratosphericusBacillusstratosphericusBacillussafensis。3324-6Paenibacilluspeoriae。01ChinaBiogas2012306347-B3++++++1-4+++++2-7+++++++4-6+++++++++8-2++++9-9++++10-5+++++10-8++++3-3－+++3-1+－+－3-4－－++7-5－－++8-6++++－9-1-2++－－9-7++－－10-3－－++10-6++－+－12-3+－+－12-4+－+－12-5+－－+2-2－－+－2-4-1+－－－2-4-3+－－－1-4－++－－4-4－－+－7-4+－－－8-8－－－+2-6+++－++－9-6-2－－－+3-9－－+++－10-2++－－－12-2－－－+“+”“－”21．J．2008484－87．2．J．200928114－20．3．、J．Vegetables2011．755－57．4．J．20115470－475．5．J．201139169683－9686．6．J．2011224100