单分子力谱-张文科

4a112003e,(,130023.,,Email:[e-mail]),.,,,.AFM,,(femtonewton),.,,[l~9].l.,,.,.,.1(AFM).AFM,1.AFM:,z,.4096,;AFM,x,Y,z512.,.,,.,,.H.E.Ganb,1997,.c1(AFM)1l4]/PN0l~000.1~1500.1oj~1000l1510ms)100ms1ms)10sDNA,DNA,RNA,,,DNA,DNA,,,:l24!}I,6].2.,.,(),,,,,(A),2(I).,,2(B).{},,.,.,,AFM(forcecalibration),,.,;,.:900,.,.,.,,,,.,,2(C).,,.,.,.,2(D).(retracting),,,.21}500pN}U....,A........200400600/nm11,}}Uup}...200400600/nl21.2,,,,.,,,.,,,31].,.,FLf/L.,Lf,L.,.,4}.1114{200PN.,,,,,./\2004006008001000/nm3,(FJC)(wLC)[8,2}.,,,..,,:,,,..(),,I20J.,,AFM.,,.,,,.,(indentationforce).,.,,,,2.1.1DNADNA,.,DNA,DNA.,RiefDNA.4.DNA,,DNA.,DNA.,DNA65pN(unwinding).Bs,DNAB(BDNA)s(sDNA),.(65pN),,.,.BS,DNA,1.52.0.BS,,(melting).,4.,,..,.,DNA,4.,()..,||!,,,2iJ.DNA0UOnn064,”100200300/nm4DNA[21]rŽKrauthauer122,2,},()l)DNA.DNA,DNAAFM,DNA.DNABS.:650pN,.,,,,.DNA,poly(dAdTpoly(dAdT),.,DNAG.().,DNA.2.L2DNA,.,,,AFM1241,DNA,,300pN.5t.,,,5().0.1mol,,,.,,.,ta1,4.a1,4,,5,251.,126.t,,26].l}l0pNO,8st0.1mol()()AFM,().,.,,400pNDNA1116[2,}.200pN,,6..,,8mol/L,,{3.,0.2mol/LNael5molzL,NaCI,.200400600/nm60.2mol.,800NaCI2.2,,.,,().,(domainStructure).(energylandscape),.,,.Rief128}AFM,.,,Rief48194198.,,.,,(48).48,.128.7.,712.,,,.89100,,,.723.,l412.,,4,,().,]7.,,,.,,.,,,,,.Rief.,;1.,Femandez127128,[29].,,.200pN4,127,300pN4128.,,.,.,,Fernand[303}.,.,Marszaekt,lx27..,[33}.,.,L(meehaniealphyenotypes).,,.oesterheltI7]AFM,,.100~200pN.,18[7}.3,Decher/(LBL),LBL!5,7}.LBL,,.,/,.,,,.3.1Rubner1381[,91997.,(PvP)/[39~4],,la2}.,AFMt,,5,25,27].AFM,().8(b)PvP,.,,.;(Ioopstructure).Ullman1961:A=(A)(a,0a1)[43.a,(trainlike);a1,(brushlike);0.1a03,.K,,,K.,a,a.,PvP256nm(),PvPa0.19142].,PvP..500PNZ20406080/nm1001201408(a),(b),,,,8(a).PvP/,PvP,PvP,,,.,t42}.,,180pN,.,,.144}HauPt145],.,ƒUnIJOJ4l..PvP,.?.,,(PNIPAM)(PS)(PNlpAMsegPS)146}.,/,.3.2,.,,?Hugcl(PvAm)lj..(trainlikestructure).,.,(PDDA)147.,,,9.,.,,[47l,.,,Senden,:50pN1009100200300400/nmPDDA148,49.,:(),,,,,(tailStrueture);;,10.,,,145}.44.1,.1994Gaub(biotin)(avidin)[50.,Si3N4,.,,;,,,,.1120(biotinavidin)(16020)pN.,(desthiobiotinavidin)(12520)pN,(iminobiotinavidin)(5515)pN[,01.,Allen[5],Hierdorfer152}.ReinhoudtVancso-[531.-p(5610)pN.,,,(0100nm),.,(spacer).4.2,.5F,DNA,DNA,F..DNAAFM,,1115457].LeeAFMDNA!54}.,.Strunz10,2030DNA155].,DNA,11.,strunzDNA16000pN/s2050pN.,.,,[56}.Pe10DNA157}.,GaubLNA(lockedmicleicacid)DNA.LNARNA,RNA24.”””11AFMDNA(a)(b)158}.,LNA,.,DNA.,LNA,158..,(unzipPing).35,(unziPpingforee).EssevazRoulet[5,},DNA10pN.,(20800nm/s).,GCAT.,RiefDNA(hairpinstructure),poly(dGdC)poly(dAdT)(203)(93)pN12}.,EsscvazRouletRief.,AFM16},162].55.1,,.(PEG).,Oesterhelt63].12PEG,:,,;100200pN,;250pN,.,,..,,l)..,20021122].,,,,./nm12x63]d/148mol...13PEG5.2,[44].148mol/L,.,,;8mol/L,,.,(,).,,[l3].,(PDMA)(PDEA)165].,,,.,8mol/L,[65.,8mol/L,PDMAPDEA165.,PDMAPDEA.PDMA2mol\PI)MA8mol/L,!\500PNPDMA15PDMA,.,.,,,5,,5,26,66],{.,,,.6,,,.,,.,,.,,.,,,.,,,.,[707,].,.,,;.,,.,.(:eZ0007sloZ)(:50073009).JanshoffA,NeitzertM,Oberd6rferetal.ForeesPeetroseoPyofmoleeularsystemssinglemoleeulesPeerroseoPyofPolymersandbiomoleeules.AngewChemIntEd,2000,38:32123237BustamanteC,SmithSB,LIPhardtJ,etal.SinglemoleeulestudiesofDNAmeehanies.CurrOPinStrue*Biol,200(),10:279~285StriekTR.AllemandJF,BensimonD,etalStresindueedstrueturaltransitionsinDNAandProteins.AnnuRevBioPhysBiomolStruet,2000,29:5235431124]l12l314l5]6l718l9202lClausenSehaumannH,SeitzM,KrautbauerR,etal.ForcesPeetroseoPywithsinglebiomoleeules.CurrOPinChemBiol,2000,4:524~530MerkelR.ForeesPeetroseoPyonsinglePassivebiomoleeulesandsinglebiomoleeularbonds.physiesReports,2001,346:343355SendenTJForeemieroseoPyandsurfaeeinteraetions.CurrOPininColloid&InterfaeeSei,2001,6:95~101YIPCM.AtomieforeemieroseoPyofmaeromoleeularinterae-tions.CurrOPinStruetBiol,2001,11:567~572HugelT,SeitzM.ThestudyofmoleeularinteraetionsbyAFMforcesPectroseoPy.MacromolRaPidCommun,2001,22:989~1016AllisonDP,HinterdorferP,HanWH.BiomoleeularforeemeasurementsandtheatomieforeemieroseoPe.CurrOPininBioteeh,2002,13:47~5l,,,,.,1995,44445,,,AFM.,2000,10:385390LiHB,ZhangWK,ZhangX,etal.SinglemoleeuleforcesPectrosc0PyonPoly(vinylalcohol)byatomieforeemieroseoPy.MaeromolRaPidCommun,1998,19:609~6]lLiHB,ZhangWK,XuWQ,etal.HydrogenbondinggovernstheelastieProPertiesofPoly(vinylaleohol)inwater:single-moleculeforcespeetroseopiestudiesofPVAbyAFM.Maeromoleeules,2000,33:465~469LiHB,LiuBB,ZhangX,etal.SinglemoleeuleforeesPeetroseoPyonPoly(aerylieaeid)byAFM.Langmuir,1999,15:2120~2124RiefM,oesterheltF,HeymannB,etal.SinglemoleeuleforeesPeetroseoPyonPolysaeeharidesbyatomieforcemiero