葡萄籽中原花青素提取工艺

892011,Vol.32,No.2011,3211,*(1.2250002.2250093.541006)1:25(g/mL)80%40s115W10.70%OptimizationofProanthocyanidinExtractionfromGrapeSeedXIAOLi-xia1HEZhi-gui1,3ZHUYong2ZENGQing-wen1HUBo-ran1,*(1.CollegeofFoodScienceandEngineering,YangzhouUniversity,Yangzhou225127,China2.CollegeofBiologicalScienceandTechnology,YangzhouUniversity,Yangzhou225127,China3.GuilinInstituteofTourism,Guilin541006,China)AbstractTheaimofthisstudywastooptimizetechnicalconditionsforultrasonic-assistedethanolextraction,cellulasehydrolysis-basedextractionandmicrowave-assistedethanolextractionofproanthocyanidinsfromgrapeseedpowerusingone-factor-at-a-timecoupledwithorthogonalarraydesignmethod.Microwave-assistedextractionwasfoundtobethebestofthethreemethods,andtheoptimalprocessconditionswereethanolconcentrationof80%,material-to-liquidratioof1:25(g/mL),microwavetreatmenttimeof40sandmicrowavepowerof115W.Undertheseconditions,theextractionrateofproanthocyanidinswas10.70%.KeywordsgrapeseedproanthocyanidinsmicrowavecelluloseultrasonicR284.2A1002-6630(2011)20-0089-062011-05-31(070121ZDXM20100005)(1966)[e-mail]*(1964)[e-mail][1-6][7-8][9-11][12-14][15-16]2011,Vol.32,No.2090311.11.2V-1600PCFW135RE52-4DL-120BTG16A-WS1.31.3.13048h1.3.2-[17]1.0mg/mL02468mL10mL0.5mL2.5mL30g/L-2.5mL30%-3030minA500nmy0.5839x0.0071(yxR20.9990)1.3.31.0g0.5mL3X0.5V2PC/%mV1103PC/%X/(mg/mL)V1/mLV2/mLm/g1.3.43322.1a.987654/%(g/mL)1:101:151:201:251:30b./%/%3040506070809087654c./%/s30405060709.08.58.07.57.06.56.0d./%/W501001502002503003507.987.777.567.357.146.936.726.51912011,Vol.32,No.202.1.11a1:208.02%1:202.1.21b70%7.3%70%2.1.31c50s50s2.1.41d115W7.85%115W2.1.51e2%32.1.6L9(34)1A(g/mL)B/%C/sD/W11:1560406521:20705011531:2580601651Table1Factorsandtheircodedlevelsinorthogonalarraydesignforoptimizingmicrowave-assistedproanthocyanidinsextractionABCD/%111112.95212223.14313333.76421237.93522318.166231210.67313210.68321310.4933217.84K198.553215.160240.333189.507K2267.315217.407189.150244.110K3289.149222.369225.537221.400k132.85171.72080.11163.169k289.10572.46963.05081.370k396.38374.12375.17973.80063.5322.40317.06118.201A3B3C1D22(n=3)Table2Orthogonalarraydesignandcorrespondingexperimentalresultsforoptimizingmicrowave-assistedproanthocyanidinsextraction(n=3)2A3B3C1D280%1:2540s115W10.70%2.21Fig.1Effectsofvariousmicrowave-assistedextractionparametersonextractionrateofproanthocyanidinse.876543210/%012342011,Vol.32,No.20922.2.12a300U/mg300U/mg2.2.22b1.5h1.5h2.2.3pHpH2cpH4pH42.2.42d505050502.2.5pHL9(34)3A/(U/mg)B/hCpHD/11001.04.04523001.55.05035002.06.0553Table3Factorsandtheircodedlevelsinorthogonalarraydesignforoptimizingcellulasehydrolysis-basedproanthocyanidinsextractionABCD/%111118.77212222.47313333.66421233.75522318.14623122.57731322.38832133.66933218.41K1148.335148.278149.301252.507K2144.525142.617146.29274.181K3144.447146.409141.744110.619k149.44549.42649.76784.169k248.17547.53948.76424.727k348.14948.80347.24836.8731.2961.8872.51959.442A1B1C1D14(n=3)Table4Orthogonalarraydesignandcorrespondingexperimentalresultsforoptimizingcellulasehydrolysis-basedproanthocyanidinsextraction(n=3)a.4.24.03.83.63.43.23.0/%/(U/mg)1002003004005006007008009002.912.702.50/%/h0.51.01.52.02.53.0b.3.523.323.112.912.702.50/%pH3456c.pH2Fig.2Effectsofvariouscellulasehydrolysis-basedextractionparametersonextractionrateofproanthocyanidins6543210/%/354045505560d.932011,Vol.32,No.204pHA1B1C1D1100U/mg1.0hpH4.0458.75%2.32.3.13a60%2.61%-60%2.3.23b1:302.3.33c453.02.52.01.51.00.50.0/%/%304050607080a.b.5.04.54.03.53.02.52.0/%(g/mL)1:101:151:201:251:301:354.94.74.54.34.13.93.73.5/%1520253025/mind.5.505.004.504.003.50/%405060708090/We.3Fig.3Effectsofvariousultrasonic-assistedextractionparametersonextractionrateofproanthocyanidins543210/%123f.5.04.54.03.53.02.52.0/%/1525354555c.2011,Vol.32,No.20942.3.43d25min25min2.3.53e80W2.3.63f32.3.7A/%B(g/mL)C/minD/W1501:2520702601:3025803701:3530905Table5Factorsandtheircodedlevelsinorthogonalarraydesignforoptimizingultraonic-assistedproanthocyanidinsextraction454L9(34)56A1B3C3D250%1:3530min80W5.57%340s22.3%92.1%80%1:2540s115W10.70%[1],,.[J].,2010(1):111-113.[2]YAHARAN,TOFANII,MAKIK,etal.Mechanicalassessmentofeffectsofgrapeseedproanthocyanidinsextractontibialbonediaphysisinrats[J].JMusculoskeletNeuronalInteract,2005,5(2):162-169.[3],.[J].:,2007,45(10):985-987.[4]SHARMASD,KATIYARSK.Dietarygrape-seedproanthocyanidininhibitionofultravioletB-inducedimmunesuppressionisassociatedwithinductionofIL-12[J].Carcinogenesis,2006,27(1):95-102.[5]DASILVAJMR,RIGAUDJ,CHEYNIERV,etal.Procyanidindimersandtrimersfromgrapeseeds[J].Phytochemistry,1991,30(4):1259-1264.[6]LIXiaoli,CAIYongqing,QINHong,etal.TherapeuticeffectandmechanismofproanthocyanidinsfromgrapeseedsinratswithTNBS-inducedulcerativecolitis[J].CanadianJournalofPhysiologyandPharmacology,2008,86(12):841-849.[7],,.[J].,2004(3):22-23.[8],,.[J].,2008,16(1):39-41.[9]JACOBJ,CHIALHL.Reviewthermalandnon-thermalinteractionofmicrowaceradiationwithmaterials[J].JournalofMaterialsScience,1995(30):5321-5327.[10],,.[J].,2005,31(1):39-42