常用酿酒酵母菌株基因型

CommonlyusedstrainsThispagedescribessomeofthemostcommonlyusedyeastlabstrains.MuchoftheinformationistakenfromF.Sherman(2002)Gettingstartedwithyeast,MethodsEnzymol.350,3-41.Otherusefulpapersforstrainbackgroundinformationinclude:MortimerandJohnston(1986)Genetics113:35-43-thoroughlydescribesthegenealogyofstrainS288CvanDijkenetal.(2000)EnzymeMicrobTechnol26:706-714-comparesvariouscharacteristicsofcommonlyusedlabstrainsWinzeleretal.(2003)Genetics163:79-89-usesSFP(single-featurepolymorphisms)analysistostudygeneticidentitybetweencommonlabstrainsS288CGenotype:MATαSUC2gal2malmelflo1flo8-1hap1hobio1bio6Notes:Strainusedinthesystematicsequencingproject,thesequencestoredinSGD.S288Cdoesnotformpseudohyphae.Inaddition,sinceithasamutatedcopyofHAP1,itisnotagoodstrainformitochondrialstudies.IthasanallelicvariantofMIP1whichincreasespetitefrequency.S288Cstrainsaregal2-andtheydonotusegalactoseanaerobically.TheS288CgenomewasrecentlyresequencedattheSangerInstitute.References:MortimerandJohnston(1986)Genetics113:35-43.Sources:ATCC:204508BY4743Genotype:MATa/αhis3Δ1/his3Δ1leu2Δ0/leu2Δ0LYS2/lys2Δ0met15Δ0/MET15ura3Δ0/ura3Δ0Notes:Strainusedinthesystematicdeletionproject,generatedfromacrossbetweenBY4741andBY4742,whicharederivedfromS288C.AsS288c,thesestrainshaveanallelicvariantofMIP1whichincreasespetitefrequency.SeeBrachmannetal.referencefordetails.References:Brachmannetal.(1998)Yeast14:115-32.Sources:Biosystems:YSC1050FY4Genotype:MATaNotes:DerivedfromS288C.References:Winstonetal.(1995)Yeast11:53-55.Brachmannetal.(1998)Yeast14:115-32.FY1679Genotype:MATa/αura3-52/ura3-52trp1Δ63/TRP1leu2Δ1/LEU2his3Δ200/HIS3GAL2/GALNotes:IsogenictoS288C;usedinthesystematicsequencingproject,thesequencestoredinSGD.References:Winstonetal.(1995)Yeast11:53-55.Sources:EUROSCARF:10000DAB972Genotype:MATαX2180-1Btrp10[rho0]Notes:IsogenictoS288C;usedinthesystematicsequencingproject,thesequencestoredinSGD.AB972isanethidiumbromide-inducedrho-derivativeofthestrainX2180-1B-trp1.References:OlsonMVetal.(1986)Proc.Natl.Acad.Sci.USA83:7826-7830.Sources:ATCC:204511A364AGenotype:MATaade1ade2ura1his7lys2tyr1gal1SUCmalcupBIONotes:Usedinthesystematicsequencingproject,thesequencestoredinSGD.References:Hartwell(1967)J.Bacteriol.93:1662-1670.Sources:ATCC:208526XJ24-24aGenotype:MATahoHMaHMαade6arg4-17trp1-1tyr7-1MAL2Notes:Derivedfrom,butnotisogenicto,S288CReferences:Strathernetal.(1979)Cell18:309-319DC5Genotype:MATaleu2-3,112his3-11,15can1-11Notes:IsogenictoS288C;usedinthesystematicsequencingproject,thesequencestoredinSGD.References:Broachetal.(1979)Gene8:121-133X2180-1AGenotype:MATaSUC2malmelgal2CUP1Notes:S288cspontaneouslydiploidizedtogiverisetoX2180.ThehaploidsegregantsX2180-1aandX2180-1bwereobtainedfromsporulatedX2180References:MortimerandJohnstonSources:ATCC:204504YNN216Genotype:MATa/αura3-52/ura3-52lys2-801amber/lys2-801amberade2-101ochre/ade2-101ochreNotes:CongenictoS288C(seeSikorskiandHieter).UsedtoderiveYSSandCYstrains(seeSobelandWolin).References:SikorskiRSandHieterP(1989)Genetics122:19-27.SobelandWolin(1999)Mol.Biol.Cell10:3849-3862.YPH499Genotype:MATaura3-52lys2-801_amberade2-101_ochretrp1-Δ63his3-Δ200leu2-Δ1Notes:Containsnonrevertible(deletion)auxotrophicmutationsthatcanbeusedforselectionofvectors.Notethattrp1-Δ63,unliketrp1-Δ1,doesnotdeleteadjacentGAL3UASsequenceandretainshomologytoTRP1selectablemarker.gal2-,doesnotusegalactoseanaerobically.DerivedfromthediploidstrainYNN216(JohnstonandDavis1984;originalsource:M.Carlson,ColumbiaUniversity),whichiscongenicwithS288C.References:SikorskiRSandHieterP(1989)Genetics122:19-27.SobelandWolin(1999)Mol.Biol.Cell10:3849-3862.JohnstonMandDavisRW(1984)MolCellBiol4(8):1440-8.Sources:ATCC:204679YPH500Genotype:MATαura3-52lys2-801_amberade2-101_ochretrp1-Δ63his3-Δ200leu2-Δ1Notes:MATαstrainisogenictoYPH499exceptatmatingtypelocus.DerivedfromthediploidstrainYNN216(JohnstonandDavis1984;originalsource:M.Carlson,ColumbiaUniversity),whichiscongenicwithS288C.References:SikorskiRSandHieterP(1989)Genetics122:19-27.SobelandWolin(1999)Mol.Biol.Cell10:3849-3862.JohnstonMandDavisRW(1984)MolCellBiol4(8):1440-8.Sources:ATCC:204680YPH501Genotype:MATa/MATαura3-52/ura3-52lys2-801_amber/lys2-801_amberade2-101_ochre/ade2-101_ochretrp1-Δ63/trp1-Δ63his3-Δ200/his3-Δ200leu2-Δ1/leu2-Δ1Notes:a/αdiploidisogenictoYPH499andYPH500.DerivedfromthediploidstrainYNN216(JohnstonandDavis1984;originalsource:M.Carlson,ColumbiaUniversity),whichiscongenicwithS288C.References:SikorskiRSandHieterP(1989)Genetics122:19-27.SobelandWolin(1999)Mol.Biol.Cell10:3849-3862.JohnstonMandDavisRW(1984)MolCellBiol4(8):1440-8.Sources:ATCC:204681Sigma1278BNotes:Usedinpseudohyphalgrowthstudies.DetailednotesaboutthesigmastrainshavebeenkindlyprovidedbyCoraStyles.GranekandMagwene,PLoSGenet.2010Jan22;6(1):e1000823,establishedthatcertainlineagesoftheSigma1278BbackgroundcontainanonsensemutationinRIM15,aG-to-Ttransversionatposition1216thatconvertsaGlycodontoanopalstopcodon.Thisrim15mutationinteractsepistaticallywithmutationsincertainothergenestoaffectcolonymorphology.AnnotationoftheSigma1278bgenomeandinfo