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1,2,2,3(1.,100081;2.;3.)MechanismsofPseudomonasfluorescensRB242andRB289inBiocontrolofPhytophthoraparasiticavar.nicotianaeGUJin2gang,FANGDun2huangLITian2fei,LIUXing2zhong(InstituteofSoilsandFertilizers,CAAS,Beijing100081,China):;;:S435.72;S476:A:100529261(2004)0120076203,,,;,,,(Pseudomonasfluorescens)(PGPR),,,,,[14]RB242RB28911.1RB242RB289(Phytophthoraparasiticavar.nicotianae)1.2CA57d(«=0.5cm)10mlCA;NA48h106107cfu/ml,5000r/min5min,;1mlCA3dCA,,3:2003204207:(98A04)[4],,.[M].:,1989.[5].[M].:,1978.[6].[M].:,2001.[7]CW,GS.PCR[M]..:,2000.[8].[M].:,1990.[9],,,.[J].,2001,27(6):16-19.6720(1)76-78ChineseJournalofBiologicalControl200421.350mlCA,50,2848h(108109cfu/ml)52550ml,(«=0.5cm),3d,315d,,0420h[5],107/ml;[6]14816h22.1RB242RB289RB242RB289,,NA(1)1RB289(A)RB242(B)2.2RB242RB289NA,RB242,RB2421,39.2%RB242RB289,(1)1NA(mm)(%)(mm)(%)NA(mm)(%)(mm)(%)RB2891068.50.772.0-15.2RB2421070.0-1.472.5-5.1549.520.849.520.8563.00.860.04.0138.524.542.516.7131.039.254.5-6.9:105169.062.551.0mm(),RB242RB289,,,RB242RB289,,,,2.3RB289RB242,4h20h,,4h85%,20h95.2%,4h,RB289RB242;20h,RB242RB289(2)2.4RB242RB289(3)4h,RB242RB289771:,83.6%;8h,RB24293.3%,RB28978.0%;16h,RB24269.1%,RB28975.8%24h()(%)20h()(%)RB28911.0085.05.3062.1101.0085.024.00-71.41001.6076.022.30-59.3RB24212.3365.10.6795.2102.3365.14.3369.11002.6760.020.30-45.0CK6.6714.0034h(%)(%)8h(%)(%)16h(%)(%)RB2894.434.321.068.521.272.91.183.614.778.018.975.82.365.732.052.043.744.1RB2423.350.74.593.324.269.12.267.218.472.442.046.31.183.624.263.728.064.2CK6.766.778.23RB242RB289,,,PGPR,,;PGPR,,40%[7],HCN,,HCNWei[8]6HCN,4HCN,HCN,,HCNRB242RB289,,,,HCN,[1]WellerDM.Biologicalcontrolofsoil2borneplantpathogensintherhizospherewithbacteria[J].Annu.Rev.Phytopathol.,1998,73:463-469.[2]OrdentlichA,EladY,ChetJ.TheroleofchitinaseofSerratiamarcescensinbiocontrolofSclerotiumrolfsii[J].Phy2topathol.,1988,78:84-88.[3]BakkerPA.TheroleofsiderophoresinpotatotuberyieldincreasebyPseudomonasputidainashortrotationofpotato[J].Neth.J.PlantPathol.,1986,92:249-256.[4]SnehB,DuplerM,EladY,BakerR.ChlamydosporegerminationofFusariumoxysporumf.sp.cucumerinumasaffectedbyfluorescentandlyticbacteriafromFusariumsuppressivesoils[J].Phytopathol.,1984,74:1115-1124.[5],,.[J],,1987,17(2):90-95.[6].()[M].:,1998.[7].[M].:,1991.329-345.[8]WeiG,KloepperJW,TuzunS.InductionofsystemicresistanceofcucumbertoColletotrichumorbicularebyselectedstrainsofplant2promotingrhizobacteria[J].Phytopathol.,1991,81(12):1508-1512.8720:A.;B.:1.RB289;2.;3.RB2421;4.RB2425;5.RB24210(1040)