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葛泉波 何淑兰 毛津淑 姚康德＊( 300072) ,24,,。 ＊(G1999054305)2003-12-30,2004-05-18 (ECMs)。、/、、、/。,。。 ,,,TheInteractionbetweenBiomaterialsandCellsandSurfaceModificationofBiomaterialsGeQuanbo,HeShulan,MaoJinshu,YaoKangde＊(ResearchInstituteofPolymericMaterials,TianjinUniversity,Tianjin300072,China)Abstract Biomaterialsasartificialextracellularmatrices(ECMs)haveplayedacrucialroleintissueengineering.Topologicalstructure,hydrophilichydrophobicbalance,freeenergy,charges,functionalgroupsandbiologicspecificityrecognitionofbiomaterialssurfacegreatlyaffecttheinteractionbetweenbiomaterialsandcells.Biomaterials-cellsinteractionismainlythemoleculerecognitionbetweenreceptorsofcellsandligandsofbiomaterialssurface.Forthepurposeofenhancingcellsaffinityandspecificityrecognition,biomimeticmodificationofbiomaterialssurfaceisessential.Thispaperintroducestheinteractionbetweenbiomaterialsandcellsandseveralsurfacemodificationmethodsforbiomaterials.Keywords Tissueengineering,Biomaterials,Cell,Surfacemodification,、[1]。,,,,。,,、,。,(/),,,,、、、[2]。,[3]。,·43·http: 20051,。,。1 1.1 ,,。(、、)、、、、。Chung(PEG)(PU),PU-PEG,N-/6-(4--2-)/(SANPAH)Gly-Arg-Gly-Asp(GRGD),2h,,PU-PEG,,MTT[4]。,,,。Dalby(PS)(4-)(PBrS),,,,,[5]。,,。,[6]。Matsuzaka,8d,,;16d,,。(ALP),[7]。,。1.2 /,,。,/,Vroman[8]。,,、/,。1.3 ,,[9]。Satriano52keV,,[10]。1.4 ,,,,[11]。,(),,[12]。1.5 。,、、,,、、、·44· 20051 http:。,/,,[13,14]。,,,,。1.6 ,,,。,,,(PEO);,(message),,[15]。,,,、,RGD[16]。2 ,,、、、,,,。,,,[17]。,。,、、,。,200,(、),(),。,,,,。,(ECMs),,,ECMs。,,,、,[18]。,,,。,。(focaladhesion)。、、α-、、[19]。(integrin),ECMs,、、,14α8β20。ECMs,·45·http: 20051[20]。,,。,ECMs,[21]。3 ,。,,,。3.1 ,(、、/)。。、,。,。YangRGD-(PLA-PLL),,、[22]。3.2 (entrapment)。(),,。(),。,,。,,[23]。,,,,。Zhu(PDL-LA)/(V()/V()=70/30),CaCl2,PDL-LA。,、[24]。3.3 。,。,,—COOH、—OH、—NH2,。,。Klee4-650℃,18℃(PVDF),(HDI),(FN),,[25]。Cui(PLLA),(EDC/NHS),(CS),,,PLLA[26]。3.4 γ(60Co)、β(85Kr)、α(210Po)X。,,,,,。,,,,。Kwon,(EPMA)(PP),·46· 20051 http:[27]。,、。MirzadehCO2KrF(PET),,L929,[28]。3.5 ,。200～400nm,300～600kJ/mol,,,。,:(1),,50～100nm,;(2)。,,,。Zhu(PMAA)(PCL),(EDC),PCL,PCL96h[29]。3.6 ,,,,。:(1);(2);(3);(4)、、。,:(1);(2)。,,。Wang(PDLLA),PDLLA3T3,[30]。Ding(Ar)(PLLA),,,。L929,[31]。3.7 ,,、,。:(1),,,;(2),,。Kim,,[32]。Chen40keV,,,,[33]。4 ,。,·47·http: 20051,ECMs。,,,[34]。,,。[1] RLanger,JPVacanti.Science,1993,260(5110):920～926.[2] ,..:,2003:274～275.[3] LLHench,JMPolak.Science,2002,295:1014～1017.[4] TZChung,DZLiu,SYWangetal.Biomaterials,2003,24:4655～4661.[5] MJDalby,SChilds,MORiehleetal.Biomaterials,2003,24:927～935.[6] LChou,JDFirth,VJUittoetal.Biomed.Mater.Res.,1998,39:437～445.[7] KMatsuzaka,XFWalboomers,JERuijteretal.Biomaterials,1999,20:1293～1301.[8] .,1997,46(9):2～6.[9] GAltankov,TGroth,NKrastevaetal.Biomater.Sci.Polym.Ed.,1997,8(9):721～740.[10] CSatriano,SCarnazza,SGuglielminoetal.NuclearInstrumentsandMethodsinPhysicsResearchB,2003,208:287～293.[11] RMShelton,ACRasmussen,JEDavies.Biomaterials,1988,9(1):24～29.[12] SPMassia,JAHubbell.Biol.Chem.,1992,267:10133～10141.[13] YTamada,YIkada.Biomed.Mater.Res.,1994,28(7):783～789.[14] JHLee,HWJung,IKKangetal.Biomaterials,1994,15(9):705～711.[15] DCAlonzo.Biomed.Mater.Res.,1997,35:513～523.[16] SKouvroukoglou,KCDee,RBiziosetal.Biomaterials,2000,21:1725～1733.[17] AMRouhi.Chem.Eng.News,1999,73(3):51～59.[18] ASHoffman.ArtificialOrgans.,1992,16(1):43～44.[19] BMGumbiner.Neuron,1993,11:551～564.[20] ACEdwin,SBJoan.Science,1995,268:233～239.[21] ROHynes.Cell,1993,69:11～25.[22] XBYang,HIRoach,NMClarkeetal.Bone,2001,29:523～531.[23] NPDesai,JAHubbell.Biomaterials,1991,12:144～153.[24] HGZhu,JJi,RYLinetal.Biomaterials,2002,23:3141～3148.[25] DKlee,ZAdemovic,ABosserhoffetal.Biomaterials,2003,24:3663～3670.[26] YLCui,ADQi,WGLiuetal.Biomaterials,2003,24:3859～3868.[27] OHKwon,YCNho,JChen.JAppl.Polym.Sci.,2003,88:1726～1736.[28] HMirzadeh,MDadsetan.RadiationPhysicsandChemistry,2003,67:381～385.[29] YBZhu,CYGao.Biomaterials,2002,23(24):4889～4895.[30] JYang,GXShi,JZBeietal.Biomed.Mater.Res.,2002,62:438～446.[31] ZDing,JNChen,SYGaoetal.Biomaterials,2004,25:1059～1067.[32] JTKim,JKPark,DCLee.PolymerInternational,2002,51:1063～1065.[33] XYChen,XFZhang,YZhuetal.PolymerJournal,2003,35(2):148～154.[34] , ,.,2000,16(6):331～332.《化学通报》征稿简则见本刊网站主页http:·48· 20051 http: