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141JOUARNI,OFWUXIUNIVERSITYOFLIGHTINDUSTRYVol.141995No.l(),,o257g。、、,2.3%96.3%102%.;;0657·3210(Hyaloin。AcidHA),NDD1,3,1,4,,、、。K.MeyerJ.W.Palmer1934〔’〕,,HA,,],、。,HA,.s[`〕。HA,HA,。,、、HA。HA,,,。W.Gold[5」(Gly-cosaminoglcyanGAG).,,,。,HA。:1994030541111.17215011.20.smol/LCH3COONa419CH3COONa(A.R.)l000ml。(AlcinablueSGS)0.149,100ml0.smol/LCH3COONa,,。0.100HA,100ml,,,1.omg/ml,,。。220ml(100g/ml)HA,12ml,,;HA,12ml,,;HA,12ml0.smol/LCH3COONa,,。25IC10min,48Onm,cZm。33.1,43onn:540nm,HA,1.50400nUID20304050(nm)607080]00110120(min)000,ó-:n01aHAb2(25C)aHAb:1,480nm,HA49onm,480m,480nm。3.2,48Onm,2.2,25IC10minh2,HA,25’C10min。3.3HA,.33,,HA,251`C10min,,。3.40.10729,,100ml,。3ml,10ml,。,,,4.:=1.45103+1.8410,CHAy=0.99974030UǎU。(。C)4080120160200240280HA(9zL)34HA3.5,,,,。109.,15oml,,。O,0.20,0.40,0.50,0.60,0.70ml,A,,,A:,△AAZA,,1.4111(n:l)00.200.400.500·600·07(Al)00.0205.0400.0055.0680.090(2)00.0900.1020.2201.1350.415△A:()0.0650.0620.0670.0607.064AZ,,,,,△AA:A,,,,0.065。.003,,HA,,。3.6sml,10ml,。,lml。.22(,,)123456785CV(%)A0.1940,2000.1950.2920.1920.2000.1980,2000.1963.6xlo“1.84HA,HA。4.14.1.1HA,,50Oml。Zml,IOm!,,HA,,3.3HA(7)AA。HA(g/n、l)(g/n,l)CV(%)0.1681741780.0040.84852254055553053053512.72.0.1700.170.4L2Zml,10ml,,.44HA()HA(g)HA(g)HACV(%)28241128241198.096.3:474(:)HA(g)H(A“g)HA(g)CV(%)99.01020114.2HAtHA、、,,5.5HAt(,2)HAHA(%)(%)CV()141.942.05。42.。41.。。.37。.944.15,,hl54L7,,、J,2.4%,96.3102%,HA。,,。MeyerKetal.J.Biol.Chem.1934,107:929.,1985,2:23.,1986,4:1.,1986,5:25GoldW.Anal.Bioehem.1979,99:183114148ASimPIeSPeetroPhotometrieMethodforHyaluronicAeidAssayLiuJieZhouXinyiZhangH,ayingZhangTian(eDpt.ofFoodcSi.andEng.)AbstraetAnewProeedureforIlyaluronieaeidassay15introdueed,whieh15basedonrheabsorPtionsPeetradiffereneebetweenaleianbluedyeandsolublealeianblueGAGoftheHAeomplexes.Alinearrelationshipbetweeneoneentraionquantitiesintherangeofo2579wasobserved.ThespeetroPhotometriemethod15deseribedinthisrePortwhiehal-lowsrhesimplerapidandreprodueible.Thepreeisionandreeoveryofthismethodarewithin2.3%and96.3%102resPeetively.SubjeetwordsSPeetroPhotometry;Aleianbluedyes;Hyaluronieaeid(HA)