82Step One Plus 定量PCR仪日常维护

Module6:PCR••PCR2|LifeTechnologiesProprietary&Confidential|9/9/2011•••3|LifeTechnologiesProprietary&Confidential|9/9/2011••UUUU•U4|LifeTechnologiesProprietary&Confidential|9/9/2011•PCR••USB5|LifeTechnologiesProprietary&Confidential|9/9/2011•PCR•••6|LifeTechnologiesProprietary&Confidential|9/9/2011−WindowsStartAllProgramsAccessoriesSystemtoolsDiskDefragment−Defragmentnow7|LifeTechnologiesProprietary&Confidential|9/9/2011−20%8|LifeTechnologiesProprietary&Confidential|9/9/2011WindowsServicePack−PCRMicrosoftWindowsSDSservicepackSDS9|LifeTechnologiesProprietary&Confidential|9/9/2011••*.sds*.eds10|LifeTechnologiesProprietary&Confidential|9/9/2011••PCR11|LifeTechnologiesProprietary&Confidential|9/9/2011•UPS1.5KVA2•2025Ԩ•20-25Ԩ•20-80%••12|LifeTechnologiesProprietary&Confidential|9/9/2011••13|LifeTechnologiesProprietary&Confidential|9/9/2011••PCR14|LifeTechnologiesProprietary&Confidential|9/9/20117300/7500PCRv1.415|LifeTechnologiesProprietary&Confidential|9/9/2011ROIROI•ROI(RegionsofInterest)•SDSBlock)•PCR16|LifeTechnologiesProprietary&Confidential|9/9/2011ROIROI73007300ROI75007500ROI75007500ROI17|LifeTechnologiesProprietary&Confidential|9/9/2011ROIROI18|LifeTechnologiesProprietary&Confidential|9/9/2011ROIROISnapshot19|LifeTechnologiesProprietary&Confidential|9/9/2011ROIROI•ROI•ROI•ROIROI20|LifeTechnologiesProprietary&Confidential|9/9/2011•PCRPCR10•PCR1060Ԩ•SDS•21|LifeTechnologiesProprietary&Confidential|9/9/2011−a.Spectralpcalibrationkit)(Backgroundplate)(Backgroundplate)−b.22|LifeTechnologiesProprietary&Confidential|9/9/201123|LifeTechnologiesProprietary&Confidential|9/9/201124|LifeTechnologiesProprietary&Confidential|9/9/201125|LifeTechnologiesProprietary&Confidential|9/9/2011720007200026|LifeTechnologiesProprietary&Confidential|9/9/201172000•720007500E90000720007500E90000•27|LifeTechnologiesProprietary&Confidential|9/9/20117300/750028|LifeTechnologiesProprietary&Confidential|9/9/2011•a−a.b−b.−c.•−•29|LifeTechnologiesProprietary&Confidential|9/9/2011•••••30|LifeTechnologiesProprietary&Confidential|9/9/2011•••31|LifeTechnologiesProprietary&Confidential|9/9/2011•••SDSSDS32|LifeTechnologiesProprietary&Confidential|9/9/2011•ROI•33|LifeTechnologiesProprietary&Confidential|9/9/201134|LifeTechnologiesProprietary&Confidential|9/9/201135|LifeTechnologiesProprietary&Confidential|9/9/201136|LifeTechnologiesProprietary&Confidential|9/9/2011•••37|LifeTechnologiesProprietary&Confidential|9/9/2011••38|LifeTechnologiesProprietary&Confidential|9/9/20117300/750039|LifeTechnologiesProprietary&Confidential|9/9/2011•7300/7500−96−0.2ml−0.2ml•0.2ml40|LifeTechnologiesProprietary&Confidential|9/9/20117500/7500FastPCR-v2.041|LifeTechnologiesProprietary&Confidential|9/9/2011PCR••42PCR•InstrumentMaintenanceInstrumentMaintenanceManager−ROI−Background−Optical−Dye−RNaseP43|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•ROISttClibitiStartCalibrition44|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•ROI45|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•ROI46|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•ROI−StatusCurrent−StatusFailed47|LifeTechnologiesProprietary&Confidential|9/9/2011PCRBkd•Background−−StartCalibration−−StartRun−•−StatusCurrentCurrent−StatusFailed48|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•Optical−Optical−StartCalibration−ROIROI−StartRun−Optical•Optical•Optical−StatusCurrentStt−StatusFailed49|LifeTechnologiesProprietary&Confidential|9/9/2011PCR•−Dyeye−StartRun−50|LifeTechnologiesProprietary&Confidential|9/9/20117300/7500PCR7300/7500PCR51|LifeTechnologiesProprietary&Confidential|9/9/2011StepOne/StepOnePlusPCR52|LifeTechnologiesProprietary&Confidential|9/9/20111.Instrument→InstrumentMaintenanceManager2.SpatialStartCalibration3.ThebackgroundplateisloadedintotheinstrumentgpSTARTRUN4.PassedFailedFinish”53|LifeTechnologiesProprietary&Confidential|9/9/201154|LifeTechnologiesProprietary&Confidential|9/9/20111.Instrument→InstrumentMaintenanceManager2.BackgroundStartCalibration3.ThebackgroundplateisloadedintotheinstrumentgpSTARTRUN4.PassedFailedFinish”Failed55|LifeTechnologiesProprietary&Confidential|9/9/20111.152.2.3.95%4.10%5.56|LifeTechnologiesProprietary&Confidential|9/9/201157|LifeTechnologiesProprietary&Confidential|9/9/20111.1Instrument→InstrumentMaintenanceManager2.DyeSystemDyeCalibration3.StartCalibrationSetupPlate1(FAMdye,ROXdye,andInstrumentNormalization)isloadedintheinstrument4.NextRunStartRun52DyeCalibrationSetup5.2DyeCalibrationSetupDyePlate2(VICdye,JOEdye,andSYBRGreendye)isloadedintheinstrumentContinueCalibration58|LifeTechnologiesProprietary&Confidential|9/9/20116.59|LifeTechnologiesProprietary&Confidential|9/9/201160|LifeTechnologiesProprietary&Confidential|9/9/2011SYBRGreenFAM61|LifeTechnologiesProprietary&Confidential|9/9/2011StepOneStepOne™™62|LifeTechnologiesProprietary&Confidential|9/9/20117900PCR7900PCR63|LifeTechnologiesProprietary&Confidential|9/9/2011Questions?6464