小鼠海马剥离

step1:Thisisadorsalviewofamouse’sskull,inordertoaccessthebrainyoucancutalongthecoronalsutureandsagittalsuturethenpulloffbothsidesofparietalboneandinterparietalbonestep2:Nowtheskullisopenandyoucanseethebrainclearly,inordertogetthehippocampusyou’dbetterkeepthebraininthecranialcavitysothebrainwillnotmovewhenyoucontinuewiththefollowingsteps0step3:Inordertoexposethehippocampusyouneedtoremovethecerebralcortexcoveringit.Thefirstincisionisattheendofthehemisphere;theincisionshouldbeabout0.7mmdeepformostadultmousethatyoumightnothurtthehippocampuswhiletoexposeit.The2ndincisionisabout1.5-2mminfrontofthefirstone,thisincisionyouneedcutintothelateralventricle,bothoftheincisionsgototheventralofthebrainandmeetthere.Nowthispieceofcortexisfree,pullitup,youwillseethehippocampusjustlikeinthispicture,alsoyoucanseetheCSFintheopenedventricle.0step4:Keepworkingontheothersideofthebrainpullupbothsidesofthecortexthatcoveringthehippocampusalongtheventricle.Nowyoucanseethedorsalpartofthehippocampus.Separatetherestofthehippocampusfromthecortexcoveringitalongthesurfaceofthehippocampustowardstheventralpartofthehippocampus.01票step5:Keepworkingontheothersideofthebrainpullupbothsidesofthecortexthatcoveringthehippocampusalongtheventricle.Nowyoucanseethedorsalpartofthehippocampus.Separatetherestofthehippocampusfromthecortexcoveringitalongthesurfaceofthehippocampustowardstheventralpartofthehippocampus.0step6:Nowyouneedtofreethehippocampusfromthesurroundingtissue0step7:Hereisthehippocampuspickedoutfromthebrain.0Thehippocampalformationisabilateralstructuresandwichedbetweenthecerebralcortexandthethalamus.Figure1depictsareconstructionofthehippocampus(yellow)initsthree-dimensional(3D)positionwithintherat'sbrain.These3DreconstructionsweremadefromtheserialsectionsofLONI'sRatBrainAtlas.0