Response-to-reviewers

DearEditor,Wearesoappreciatedforyourletteronourmanuscript(NANO-D-13-00099),entitledanewfunctionofgrapheneoxideemerge:inactivatingphytopathogenicbacteriumXanthomonasoryzaepv.oryzae.Wearealsoextremelygreatfultothereviewers’commentsonourmanuscriptandcarefullyconsideredeverycomment,andmadecautiousrevisionaccordingly.Basedontheirsuggestions,wehaveansweredthequestionsindetailonebyone.Ifyouhaveanyotherquestionsaboutthispaper,Iwouldquiteappreciateitifyoucouldletmeknowthemintheearliestpossibletime.Mostsincerely,HeyouHanJuly12,2012AdditivelistToreviewer#1:Comment1:AuthorsneedtoshowthereductionpropertybetweenGOandrgothroughTEMandRamanspectroscopy.Answer:Thanksforyourverythoughtfulsuggestion.WehaveshowedanddiscussedthecharacteristicofGOandrGOthroughTEMandRamanspectroscopy.TheimageofTEMandRamanspectroscopyarecombinedtotheUVandFT-IRspectraofGOandrGO,whichisnamedFig.2intherevisedmanuscript.Comment2:Alwaysthebacterailgrowthneedtoshowinlog.Answer:Thanksforyourverythoughtfulsuggestion.Thebacterialgrowthhasbeenchangedandshowedinlog.Toreviewer#2:Comment1:Graphenesheetsmaywrapcellscausingcelldeath.Theauthorsmayalsoconsiderthismechanismintheirdiscussionpart.LateralDimensionDependentAntibacterialActivityofGrapheneOxideSheets,Langmuir,2012,28,12364−12372Answer:Thanksverymuchforyoursuggestion.IamsorrythatIdidnotdiscusstheantibacterialmechanismclearly.Wehavereadthereferencecarefullywhichyousuggestedmeandrethinkourresults.ThesuggestedmechanismhaveconsideredintheResultsandDiscussionspart,referringtothefollowingliteratures:“LiuSB,HuM,ZengTH,WuR,JiangRR,WeiJ,WangL,KongJ,ChenY(2012)Lateraldimensiondependentantibacterialactivityofgrapheneoxidesheets.Langmuir28:12364−12372”and“LiuSB,ZengTH,HofmannM,BurcombeE,WeiJ,JiangRR,KongJ,ChenY(2011)Antibacterialactivityofgraphite,graphiteoxide,grapheneoxide,andreducedgrapheneoxide:membraneandoxidativestress.ACSNano5:6971–6980”.这块你就写已经把讨论放在修改手稿中Comment2:Figure6isverydifficulttoread.Theauthorsmayconsideradjustingthecontractofdifferentcolors.Answer:Thankyouforyourcomments.WehaveadjustedthecontrastofdifferentcolorsinFigure6oforiginalmanuscripttomakethedifferentcolorsintheimagehaveasharpcontrast.Wethinkthemodificationcanmakethepicturereadasclearlyaspossible.Toreviewer#4:Comment1:TheEnglishshouldbeimprovedtomakethedescriptionmoreclearandconcise.Somesentencesareincompleteandsometypemistakesexistinthemanuscript.Anincompletesentenceexample:Adropofwithafinalconcentrationof10uMwasadded...(page6,line22).Answer:Thankyouforyourcomments.ThemanuscripthasbeenthoroughlycheckedandrevisedthemistakeswiththehelpofanativespeakerandanEnglishteacher.Comment2:ThelastparagraphintheIntrodutionlooksmorelikeanAbstractbecauseitmainlydescribestheexperimentmethodsandtheresultsotherthantheaimsandtheimportanceoftheresearch.Additionally,thelasttwoparagraphsintheResultsandDiscussionshavenocloserelationswithexperimetaldata.Theyaregeneraldescription,anditisbettertocombinethemintotheIntroductionortheConclusition.Answer:Thanksforyourverythoughtfulsuggestion.Wehaverevisedthesectionofintroductionandtheconclusioninthemanuscript.ThelasttwoparagraphsintheResultsandDiscussionswerereorganizedandcombinethemintotheIntroductionortheConclusition.Comment3:ItisdifficultformetounderstandwhyrGOpossessedhigheroxidativecapacitythanGO(page13andFigure9a)becauserGOisreducedfromGObyareducingagent.这块你理解透了没？他问为什么RGO比GO具有强的氧化性。再参考一下文献Answer:Thanksforyoursuggestion.GOiselectricallyinsulatingmaterials,whenGOisreducedbyareducingagent,theπelectronofgraphenecanbepartlyrestoredandtheelectricalconductivitycanberecovered(Zhouetal.2009).Therefore,hydrazinereductionofGOledtoablackrGOsuspension,amoreconductiveversionofGOnanosheetswithlesssurfacedefects.Then,conductivityofrGOishigherthanGO.Glutathione(GSH),atripeptidewiththiolgroups,ispresentathighlevelsinmostGram-negativebacteria.rGOcanactasaconductivebridgeovertheinsulatinglipidbilayerreleasingcellularenergy(2GSH=GSSH+2e-+2H+)intotheexternalenvironment(O2++2e-+2H+=H2O2),whileGOisnotasgoodasrGO.Then,rGOwithhigherconductivityshowshigheroxidationcapacitiestoGSHthanGOwithlowerconductivity(Liuetal,2011).ItcanexplainthatrGOpossessedhigheroxidativecapacitytoGSHthanGOinfigure9a.Comment4:PleasegivethediscussionrelatedtotheFT-IRspectra,andexplainwhytheFT-IRspectraofGOandrGOcandemonstratethefullyoxidationofgraphiteandreductionofGO(page8).Answer:Thanksforyoursuggestion.WehaveaddedthediscussionrelatedtotheFT-IRspectraintherevisedmanuscriptandgiveanexplanationthathowcanitbeseenfullyoxidationofgraphiteandreductionofGOfromtheFT-IRspectra.Toreviewer#5:Comment1:Xanthomonasoryzaepv.OryzaechangetoXanthomonasoryzaepv.oryzae,trytofollowsamestylethroughoutthemanuscriptAnswer:Thanksforyoursuggestion.WehavechangedXanthomonasoryzaepv.OryzaetoXanthomonasoryzaepv.oryzaethroughoutthemanuscriptaccordingtoyousuggestion.Comment2:Page2,line8,organsulfurschangetoorganosulfursAnswer:Thanksforyoursuggestion.Wehavechangedorgansulfurstoorganosulfursintherevisedmanuscipt.Comment3:OsO4changetoOsO4Answer:Thanksforyoursuggestion.DoyoumeanOsO4shouldbechangedtoOsO4?I