大鼠心肌缺血再灌注模型构建的改良

20105205CHINESEJOURNALOFCOMPARATIVEMEDICINEMay2010Vol.20No.5檵檵檵檵檵檵檵檵檵檵殝殝殝殝200821117。1984－。[e-mail]、、、。[e-mail]1212341.8320002.8300003.8300544.830002【】。32SDABC30minD60min。72h。91%。B、C、DAP0.05C、DBP0.05。B、C、DAP0.05C、DBP0.05。。【】【】R542.2【】A【】1671-7856201005-0024-05ImprovementofEstablishingaMyocardialIschemicReperfusionModelinRatsCHENBing-xin12RENPeng12DUANMing-jun3TIANFei41.ShiHeziMedicalCollegeShehezi832000China2.PeopleHospitalofXingjiangUrumqi830000China3.FirstAffiliatedHospitalofXingjiangMedicalUniversityUrumqi830054China4.ThirdPeopleHospitalofXingjiangUrumqi830002China【Abstract】ObjectiveToimprovethemethodofestablishingmodelinmyocardialischemicreperfusionrat.Methods32SDmaleratsweredividedintofourgroupsrandomlyGroupAshamgroupGroupBmyocardialischemicGroupCischemic30minandreperfusionGroupDischemic60minandreperfusion.InspectedthechangingofECGinpreparation/in-operationandratsbreathingwerefinishedunderthecontrolofartificialrespiratorintheperiodofMI.After72hoftheoperationthelevelsofmyocardialzymogramweredeterminedandtheleftventricularpressurewastraced.ResultsSurvivalratewas91%afteroperation.MyocardiumzymogramcontentinthegroupBCDwassignificantlyhigherthanthatofgroupAP0.05MyocardiumzymogramcontentingroupC、DwaslowerthanthatofgroupBP0.05.TheleftventricularpressureingroupBCDwaslowerthanthatofgroupAP0.05TheleftventricularpressureingroupC、DwashigherthanthatofgroupBP0.05.ConclusionTheimprovingmethodisconvenienthighersurvivalrateandtheevaluationwasaccordingwithclinicalprocedures.【Keywords】ModelanimalIschemia-reperfusionMyocardialRat、1。。、、。、。11.11.1.1SD32220～260gSYXK2003-0001。AB72hC130minD260min。1.1.2HX-200、ECG8951DBeneviewT5、CarlZEISS、、16、10cm1.5mm、245mm、50cm40cm、、、、、12。1.1.310%。1.21.2.13d12h。10%0.32mL/100g1cm。、。、。1.2.23、44、52cm、3、44、560/min112mL/100g。3min3、、7-0leftdescendinganteriorbranchLAD。。。。8。3。、。。15。。。1.2.3。STTQRSSTTQRS。1.31.3.1、5min、30min、60min、5min、15min、30min。P、QRS、T、ST。1.3.272h。1.3.330minCreatineKinaseCK、5220105205ChinJCompMedMay2010Vol.20.No.5CreatineKinase-MBCK-MB、AspartateAminotransferaseAST、LactateDehydrogenaseLDH。1.4SPSS13.0。±LSD-t。22.13212.5%B2CD3。。、。242291%。2.2PQRSB、C、D2410P、、640minP。24R16QRS5minR。PR6QRS。QQ。A。STT24LAD4STT。STT。A3ST。2216、2。2Fig.2ThechangingofECG0.1.5minQRSST2.15minQRSST3.30minST4.60min55min615minQRSST7.30minq。Note0.nomalECG.1.5minafterischemicthehigherofQRSandtheraisedofSTsegment.2.15minafterischemicthefurtherchangeofQRSandSTandthelowerofheartrate.3.30minafterischemicthesignificantlyraisedofSTsegment.4.60minafterischemictheappearanceofventricularbegeninalrhythm.5.5minafterreperfusionthedisappearanceofprematurecontration.6.15minafterreperfusiontherecoveryofQRSandSTsegment.7.30minafterreperfusiontheappearanceofsmallpathologicalqwave.6220105205ChinJCompMedMay2010Vol.20.No.52.3B、C、DCK、ASTB、DCK-MB、LDHAP0.01CCK-MB、LDHAP0.05C、DCK、CK-MB、LDH、ASTBP0.01CCK、CK-MB、LDH、ASTDP0.011。1AST、CK、CK-MB、LDHTab.1ComparisonofthelevelofplasmaASTCKCK-MBandLDHinvariousgroupsGroupnASTCKCK-MBLDHAGroupA87.625±3.962A60.000±44.372A57.506±45.673aB92.000±37.443aBBGroupB699.500±13.457B933.333±175.861B784.683±189.358A517.167±104.526ACGroupC757.714±8.770C285.000±140.219C223.229±124.153bB205.429±94.336bBDGroupD778.000±4.583D615.000±174.207D518.614±124.523C417.857±86.012CP0.05P0.01。NoteValuesinsamecolumnswithsmalllettersmeanP0.05withcapitallettersmeanP0.01.2.4A、B、C、D120.875±11.763mmHg、63.833±17.680mmHg、85.857±13.397mmHg、83.857±15.159mmHg。B、C、DAP0.01C、DBP0.05C、DP0.05。3-。。。1.。。、3。、、。、23。4、。2.。。。3.。。、、。4.。。5.、。。TQRSSTSTT、ST。Q。5。QRS。QRSQRS。630min。73%6。Evan’s、TTC。、7220105205ChinJCompMedMay2010Vol.20.No.57。30min60min。。30min60min。30min60min。I/R、、。。PCI。。。1.J.20071710576－580.2.J.2007149－51.3./J.2007113206－208.4./J.2006379980－982.5.-J.2006198700－702.6HansenPR.MyocardialreperfusioninjuryexperimentalevidenceandclinicalrelevanceJ.EurHeartJ199516734－740.7.-J.2006196524－529.〔〕2009-11-26231LeeBH.KimWHChoiMJetal.Chronicheartfailuremodelinrabbitsbasedontheconceptofthebifurcation/trifurcationcoronaryarterybranchingpatternJ.ArtifOrgans2002264360－365.2DegabrieleNMGfiesenbachUSawKetal.CriticalappraisalofthemousemodelofmyocardialinfarctionJ.ExpPhysic12004894497－505.3TheJointEuropeanSocietyofCardiology/AmericancollegeofCardiologyCommittee.MyocardialinfarctionredefinedAconsensusdocumentoftheJointEuropeanSocietyofCardiology/AmericancollegeofcardiologyCommitteefortheredefinitionofmyocardialinfarctionJ.JAmCoilCardial200036950－969.4.M.2000462.5.J.2004122218－220.6BodorGSPoterSLandtY.ThedevelopmentofmonoclonalantibodiesandanassayforcardiactroponinIwithpreliminaryresultsinsuspectedmyocardialinfarctionJ.ClinChem1992112203－2214.7WuAH.AcomparisonofcardiactroponinTandcardiactroponinIinpatientswithacutecoronarysyndromesJ.CoronArteryDis19991069－74.〔〕2009-12-048220105205ChinJCompMedMay2010Vol.20.No.5郭亚南，等.DNCB致敏/激发Nc/Nga小鼠特应性皮炎模型的建立（正文见第12页）GUOYa-nan,etal.EstablishmentofAtopicDermatitisModelwithNc/NgaMiceSensitizedandChallengedwithDNCB(Fulltextonpage12)图1模型组和对照组小鼠皮损炎性反应比较Fig.1Thecomparisonofskin