污染水中铜离子浓度的快速测定-杨润萍

[]　(1966-),,,,。【】污染水中铜离子浓度的快速测定杨润萍1,李晓霞1,丁磊2,陈微1,金光霞1,张冬梅1(1.,　110141;2.,　110141)[]　:。:pH=9,,1:2,452nmA,。:0.10～2.5μg/ml,A,r0.9999,95.8%～102.9%,。:,,。[]　铜试剂;铜;污染水[]　R123.1　　　　[]　A　　　　[]　1004-8685(2007)12-2217-03DeterminationofcopperionsinpollutedwaterYangRun-ping1,LiXiao-xia1,DingLei2,ChenWei1,JinGuang-xia1,ZhangDong-mei1(1.YuhongCenterforDiseaseControlandPrevention,Shenyang110141,China;2.YuhongInstituteofHealthInspetion,Sheny-ang110141,China)[Abstract]　Objective:Toestablishthedeterminationofcopperionsbyusingspectrophotometricmethod.Methods:Intheam-moniumhydroxidewithpHvalue6.2,copperionseffectedwithdithiocarb,andproduceyellow-ambercolloidcompound.Themoleratiowas1:2.TodeterminevalueAinthewaveof452nm.Toquantitatetheconcentrationofcopperionsbystandardcurve.Results:Whentheconcentrationofcopperionsisbetween0.10and2.5μg/ml,thelinearrelationshipisgoodbetweenAandtheconcentrationofcopperions,thevalueofcoefficientcorrelation,ris0.999,recoveryrateis95.8%～102.9%,thede-greeofaccuracyandprecisionishigh.Conclusion:Itiseffectivetodeterminethecopperpollutedwaterbythismethod,andthedegreeofaccuracyishigher.Sothemethodisfeasible.[Keywords]　Biscyclohexanoneoxaiyldihydraone;Copper;Pollutedwater　　,,。。,,,、,。、、。、,,。1　1.1　实验原理pH=9,(,DDTC-Na),1:2。,。452nm,,30min。1.2　仪器与试剂722、。:1000μg/ml;10μg/ml;(27μg/ml);(54μg/ml);;()。2　2.1　铜配合物的最大吸收波长的测定5.00ml10μg/ml50ml,20.00ml27μg/ml,。340～460nm,16,A。:,452nm,452nm。2.2　反应时间对吸光度值的影响5.00ml10μg/ml50ml,20.00ml27μg/ml,,452nm。5min40min9。:,,22172007121712　ChineseJournalofHealthLaboratoryTechnology,Dec2007;Vol17　No1230min,5～25min。2.3　pH值对反应的影响1～95.00ml10μg/ml,20.00ml27μg/ml,0.20,0.50,0.80,1.00,2.00,3.00,4.00,5.00mlpH,,452nm。:pH。,pH9,。2.4　铜试剂用量对吸光度值的影响1～650ml5ml10μg/ml,5.00,10.00,15.00,20.00,25.00,30.00ml27μg/ml,:=1:1,1:2,1:3,1:4,1:5,1:6,,452nm。:2:1,,。2.5　干扰实验、、、、。:、0～250μg/ml,0～10μg/ml,0～0.3μg/ml;0～0.4μg/ml,。,,。2.6　检出限的测定20ml27μg/ml50ml,pH9,,452nmA,10。0.031μg/ml,0.10μg/ml。2.7　标准曲线的绘制1～1650ml0.20,0.30,0.40,0.60,0.80,1.00,2.00,3.00,4.00,5.00,6.00,7.00,8.00,10.00,15.00ml10μg/ml,,pH9,,452nm,。1　ρ(μg/ml)00.040.060.080.120.160.200.40A00.0060.0100.0150.0200.0280.0330.068ρ(μg/ml)0.600.801.001.201.401.602.002.50A0.1000.1320.1650.2000.2350.2680.3280.477:0.10～2.50μg/ml,A,A=0.1773ρ-0.0045,r=0.9999;,,,,,。2.8　精密度准确度的测定2.8.1　精密度的测定　1550ml,3,3,7,12.5ml10μg/ml,,pH9,,452nm,5。2　12345sRSD(%)A0.1000.1020.0980.1000.1021.67×0-31.67A0.2340.2360.2350.2350.2331.14×0-30.48A0.4770.4780.4750.4770.4761.26×0-30.26:,0.1～0.5,。2.8.2　准确度的测定　1～550ml30ml,3.60、3.90、4.20、4.50、4.70ml20μg/ml,,pH9,,452nm。3　(ml)3.603.904.204.504.70(μg)7278849094(μg)6976.98692.691.7(%)95.898.6102.4102.997.6:95.8%～102.9%,,,。2.9　水样分析500ml,pH=1-2,,,。30.00ml50ml,10.00ml27μg/ml,pH=9,,452nm。8。4　n12345678A0.0090.0090.0080.0090.0100.0090.0090.009ρ(μg/ml)0.0760.0760.0710.0760.0820.0760.0760.076C0.076RSD(%)5.94,0.076μg/ml。RSD=5.9%。(2345)22182007121712　ChineseJournalofHealthLaboratoryTechnology,Dec2007;Vol17　No12,2,。12,51～6。1　200722-+++-+++++-------------------------　　+,-2　5(min)5.226.108.2412.3117.17(%)　99999999941　1、2、3、4、5:、、、、2　3　4　5　6　　　,、、3,。,、、3,。[][1]GB/T19648-2005.446[S].[2]GB/T19649-2005.405[S].(:2007-07-23)(2218)3　,0.10～2.5μg/ml,。。[][1],,.[M].:,1981.286-289.[2]()Z,.,,,.[M].,1983.55-58,214-216.[3].,.,.[M].,1981.122-124.[4]《》.[M].,1989.24-30,262-264.(:2007-09-03)23452007121712　ChineseJournalofHealthLaboratoryTechnology,Dec2007;Vol17　No12