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X,(,641112):,2DE:;;;:Q51:A:1671-1785(2003)02-0045-04,,:,,(Proteome)WilkinsWilliams1994[1],,,,1,,,(),:1);2);3);4);5),2DE,(:Melanie3,ImageMaster2D),(massspectrometry,MS)(ESI-MSMALDI-TOF-MS)1111000-3000(spot)[3],106,(),[4],,,182(2003)JOURNALOFNEIJIANGTEACHERSCOLLEGENO121Vol118(2003):20021213:(1975-),,,112(2-DE)(IEF),(PI);SDS-(SDS-PAGE),,,10-150ku80PH(IPG)[5],,,2-DE2-DF,,2-5ngPI,,,(PDque),,2-DE[2];1);2);3);4);5),11311311,,[6],,Edman,,,,,11312(MS),(ESI)(MALDI)[7],,,,(PMF)[8]()2-DE,C-,(MALDI-TOF-MSESI-MS)(Tan2dem-MS)[9],-N(B)C(T),,NC,Mann[10],NC114-,[11]11114[12],,DNA(DB)(AD)DBAD,Gal4()DB,DBAD,,64182(reportgene),1)2),,,,,,11412(SPR)[13]SPRSPR(),,,SPR,SPR[14]1);2),,,2,,:[15]:Humphery-Smith(proteomiccontig)[16],PH,,,,;(OSDT)(PIGD)(DPD)2DE,MALDI-TOF-MS;(MLCTMS)[17]2DEPI,:(ICAT)[18]2DE,[19],,,,,[20],,,[21],,,,,,[1]WilkinsMR,PasqualiCetal.FromProteinstoProteomes:Largescalcproteinidentificationbytwo2dimen2sionalelectrophoresisandaminoacidanalysis[J].BioöTechnology,1996,14:61-65.[2].[J].,2001,17(15):491-493.[3],,.[J].,1998,18(6):4-6.[4],.[J].,2001,28(3):415-417.7471,:[5]BjellqvistB,RighettiP.Getal.IsoelectricfocusinginimmobilizedpHgradients:Principle,methodologyandsomeapplications[J].J.BiochemBiophysMethods,1982,6:317-319.[6]HumpherySmithI.,CordwellS.J.,BlackstockW.P.Proteomeresearch:complementarityandlimitationswithrespecttotheRNAandDNAworlds[J].Electrophoresis,1997,18(8):1217-1242.[7],.[J].,2001,4:38-41.[8]HenzelW.J.,BilleciT.M.,WatanceC.etal.Identifyingproteinsfromtwo2dimensionalgelsbymolecularmasssearchingofpeptidefragmentsinproteinsequencedatabases[J].Proc.Natl.Acad.Sci.USA,1993,90:5011-5015.[9],,.ö[J].,1999,31(4):425-432.[10]MannM.etal.Error2tolerantidentificationofpeptidesinsequencedatabasesbypeptidesequencetags[J].Anal.Chem,1994,66(24):4390-4399.[11]Mee2JungHan,SangSunYoon,SangYupLee.ProteomeAnalysisofMetabolicallyEngineeredEscherichiacoliProducingPoly(32Hydroxybutyrate)[J].J.Bacteriol,2001183:301-308.[12],.[J].,1999,31(3):221-225.[13],.TNF[J].,2001,17(4):375-379.[14].-(BIA)[J].,1997,24(1):91-94.[15]WilkinsMR,SanchezJC,WilliamsKL,HochstrasserDF.Currentchallengesandfutureapplicationsforpro2teinmapsandpost2translationalvectormapsinproteomeprojects[J].Electrophoresis,1996;17:830-838.[16]HumpherySmithI.,BlackstockW.Proteomeanalysis:genomicsviatheoutputratherthantheunputcode[J].J.Prot.Chem,1997,16(5):537-544.[17],.:[J].,2001,33(5):477-487.[18]WashburnM.P.,WoltersD.etal.Large-scaleanalysisoftheyeastproteomebymultidimensionalproteinidentificationtechnology[J].Nat.Biotechnol,2001,19(3):242-247.[19]Gygisp.R.GerberS.A.,TurecekF.Quantitativeanalysisofcomplexproteinmixturesusingisotope2codedaffinitytags[J].Nat.Biotechnol,1999,17:994-999.[20],,.[J].,2001,21(5):410-411.[21],,.[J].,2001,28(2):164-167.ResearchandProspectofProteoneLIUYong,ZHANGJun2Hua(DepartmentofChemistry,NeijiangTeachersCollege,Neijiang,Sichuan641112,China)Abstract:Theproteomics,whichcharacterizes,quantify,analyzeallproteinsinagenomeatlevelofcellularinaglobalway,therefore,isoneoftheactiveresearchfieldsinthePost2GenomeEra1TheapplicationofproteomicsProvidesmajoropportunitiestoelucidatediseasemechanisms,identifynewtherapeuticstargets,veiloriginoflife,etc1Themaintechniquesusedinproteomicsaremainlytwo2di2mensionalelectrophoresismass2spetrometry,yeasttwo2hybridsystem,bioinformatics,etc1Keywords:proteone;proteomics;two2dimensionalelectrophoresis;mass2spetrometry84182