奶牛发情周期中生殖激素浓度与卵泡发育对比分析

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1.,833411;2.,712100;3.,833419;4.150,832015):221d10,(RIA)17,B2.:FSHLHE2,12().:;;:S823.9+1:A:100324315(2008)0320042205ComparisonofconcentrationsofreproductivehormoneandovumgrowingduringoestrouscycleofdairycowsLIGui2zhi1,LIFei2,DIAOXiu2nian1,HUXu2sheng4,MAHui2ming2(1.No.81Regiment,No.5DivisionofMilitaryFarm,Bole833400,China;2.NorthwestAgriculturalandForestrySci2TechUniversity,Yangling712100,China;3.No.91Regiment,No.5DivisionofMilitaryFarm,Bole833419,China;4.No.150Regiment,No.8DivisionofMilitaryFarm,Shihezi832015,China)Abstract:TheconcentrationsoffourkindsofreproductivehormoneofFSH,LH,P4andE2werede2terminedbyradioimmunoassayintheperipheralbloodduringtheestrouscycle.Theovumgrowinginheat2ingwasmeasuredbyBmodelultrasonicwavedetector.Theresultsshowedthat2peakswerefoundinsizeofovumgrowing,andconcentrationofFSH,LHandE2duringtheestrouscycle.Itissuggestedthatfailedheatingandovulatingofcowsin12thdayoftheestrouscycle(peakofovarianfollicle)wouldberelatedwithlowerconcentrationofreproductivehormone.Keywords:dairycows;ovumgrowing;reproductivehormone.BoltDJ[1-3](FSH)(LH)(P4)17(E2):(1964-).,,.E-mail:[e-mail]:,,,.E-mail:[e-mail]:(05xz05),:.:2006209205,FSH,LH,.P4E2,.Campbell[4,5]23,,11.,PustaD[6,7].2008634246JOURNALOFGANSUAGRICULTURALUNIVERSITY43©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[8]11,,,.,,,.11.12004388,1002,,,,,,21d10.1.2B-(SSD-500Aloka)(5.0MHz)(OLYM2PUS).1900,1,21d,,,,.1.32004310,,9,10mL/,,4,1,1d,21d.30%50LEDTA,,3500r/min15min,,-20,30d.1.4.(P4)(E2),(RIA).,(proges2teroneRIAKit,0411)(E2-RIAKit,0415),.P4080ng/mL,0.02ng/mL,8%,12%;E201000pg/mL,0.5pg/mL.10%,16%.FSHFSHFSH[8].FSH,Greenwood(1963)-T.(USDA-bFSH-B-I)(USDA-5-Pool-Bfsh-b).FSH160000,,13.1ng/,1128ng/.LHLHLH.-T125I.LH1200000.113,NIH-LH-S18.0.1ng/,0.12516.000ng/..1.5Excel,SPSS11.0,,F.22.11,(FSH)2,10-1319-20FSH,(20.21.23)ng/mL(30.20.46)ng/mL,19-2030.2ng/mL342:©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[5].2(LH)2,121213,121312.3(P4)1,,1,192P4.17(E2)172,2,912(4).2.25,2,10-12212,1.5cm,.1FSHFig.1GraphofFSHinbloodofcow‘soestrusperiods2LHFig.2GraphofLHinbloodofcow‘soestrusperiods3P4Fig.3GraphofP4inbloodofcow‘soestrusperiods4E2Fig.4GraphofE2inbloodofcow‘soestrusperiods5Fig.5Graphoffolliculardiaincow‘soestrusperiods33.15,212,1.5cm,3442008©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[9]23,2,,12,.3.2FSH2,FSH101220FSH,.FSH11,FSH[10-12],,.15,FSH,.FSH25.230.2ng/mL,0.81.5cm,.10FSH20,11.3.3LHLH[13],.LH2,,.2LH212,2LH12,2,12,LH.3.4P43P4192,,E2P4.3,P412,,.[14].P4,.,,,(Clpros2tenol),[15],,11,,.3.5E2E2,,.4212,,E22,12E22.E2,,2121.5cm,,E2122.,[7].[1]BoltDJ,RollinsR.Developmentandapplicationofaradioimmunoassayforbovinefollicule-stimulatinghormone[J].AniSci,1983,56[2]NiswenderGD,ReichertLE.RadioimmunoassayforbovineandovineLuteinizinghormone[J].Endocrinolo2gy,1969:84[3],,.[J].,2004,39(5):5042507542:©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[4]Campbell.Humanchorionicgonadotropininducedal2terationsinovarianfolliculardynamicsduringthees2truscycleofheifers[J].J.AnimSci,1998,76:192921936[5],.[J].,1983,(1):425[6]PustaD.Persistentdominantfolliclealterspatternofoviductalsecretoryproteinsfromcowsinestrus[J].BioReprod,1999,61:1272134[7],,.[J].,1999,(4):3352336[8],,,.[J].,1998,(4):124[9]BaoB.Expressionofsteroidogenicenzymeandgonado2tropinreceptorgenesinbovinefolliclesduringovarianfollicularwavesareview[J].Sci,1998,76:190321921[10]HulshofSCJ.Resumptionoffollicularwavesinbeefcowsisnotassociatedwithperiparturientchangesinfollicle2stimulatinghormoneheterogeneitydespitema2jorchangesinsteroidandluteinizinghormoneconcen2trations[J].BioReprod,1998,58:144521450[11]AcostaTJ.LocalreleaseofsteroidhormoneprostaglandinE2,andendothelinkfrombovinematurefolli2clesinvitro:effectsofluteinizinghormone,endothelinkandcytokines[J].BioReprod,1998,59:4372443[12]FrajblatM.Plasmaleptinconcentrationsfirstpost2partumovulationindairycowsdefferinginenergybalance[J].JAnimScisuppl,1998,76:231[13]EchternkampSE.Increasedovarianfollicdevelop2mentincattleselectedfortwinbirths[J].JAnimSciSuppl,1998,76:222[14],[J].,2000,36(1):38240[15]WilkinsonC.Effectsoffanandsperlcoolingonthefollicularchemistryoftheovulatoryfollicleinlacta2tingHolsteinsduringthesummer[J].JAnimSciSuppl,1998,76:221642008