微生物细胞活性检测

TB337Promega:8008108133E-mail:[e-mail]337110BacTiter-GloTMTB337G8230,G8231,G8232G8233………………………………….5A.…………………………………………………………………………...5B.ATP……………………………………………………5C.ATP…………………………………………5.…………………………………………………………………………………..6A.BacTiter-GloTM………………………………………………………...6B.……………………………………………………………………….6C.BacTiter-GloTM…………………………………………………..8……………………………………………………….10I.BacTiter-GloTMATPATPBacTiter-GloTMHTSBacTiter-GloTM1ATPATP2BacTiter-GloTMUltra-GloTMATPBacTiter-GloTM3301ATPTB337Promega:8008108133E-mail:[e-mail]-GloTMTB337Promega:8008108133E-mail:[e-mail]25922ATCC25923ATCC27853ATCC10987MuellerHintonII(MHII)BD#297963IV37oCMHII50MHII96IIIBacTiter-GloTM1.5IVTurnerBiosystemsVeritasTM#E6501Luria-Bertani=[-]/]54015070103.Mg2+ATP4.BacTiter-GloTM2Luria-BertaniMuellerHintonIIMHIIBD#297963TrypticaseSoyTSB,BD#299113106TurnerBiosystemsVeritasTM#E6501MHIILBTSB262868TB337Promega:8008108133E-mail:[e-mail]-GloTMMicrobialCellViabilityAssay10mlG823096100µl10038425µl400•10mlBacTiter-GloTMBuffer•1BacTiter-GloTMSubsrate()•1BacTiter-GloTMMicrobialCellViabilityAssay10X10mlG823196100µl10038425µl400•10X10mlBacTiter-GloTMBuffer•10BacTiter-GloTMSubsrate()•1BacTiter-GloTMMicrobialCellViabilityAssay100mlG823296100µl1,00038425µl4,000•10x100mlBacTiter-GloTMBuffer•10BacTiter-GloTMSubsrate()•1BacTiter-GloTMMicrobialCellViabilityAssay10x100mlG823396100µl1,00038425µl4,00010,00040,000•10x100mlBacTiter-GloTMBuffer•10BacTiter-GloTMSubsrate()•1BacTiter-GloTMBacTiter-GloTM-20oCBacTiter-GloTM-20oC6BacTiter-GloTM4oC48TB337Promega:8008108133E-mail:[e-mail]337510BacTiter-GloTM8BacTiter-GloTM4oC4-20oC-70oC20%III.BacTiter-GloTMzzzzVeritasTM#E6501TurnerTD20/20#E2041,E2051CCDzATPA.1.BacTiter-GloTM48BacTiter-GloTM2.BacTiter-GloTM3.#G8230,G823110ml,#G8232,G8233100mlBacTiter-GloTMBacTiter-GloTM/BacTiter-GloTM4.BacTiter-GloTM15.15B.ATP22oC-25oC1.100µl/9625µl/3842.3.4.BacTiter-GloTM96100µl/100µl38425µl5.56.C.ATP22-25oC1.1µMATP(100µl1µMATP10-10ATP)2.ATP101µM10pM;100µl10-1010-15ATP…ATP…15IV.BTB337Promega:8008108133E-mail:[e-mail]3376103.100µlATP4.ATPBacTiter-GloTM115.1ATP,6.IV.a)BacTiter-GloTMUltra-GloTMATPPhotinuspyrailsATPPHATPPromegaPhotinuspennsylvanicaUltra-GloTMATPATP1BacTiter-GloTMATP1.BacTiter-GloTMEscherichiacoliStaphylococcusaureusSaccharomycescerevisiaePseudomonasaeruginosaEnterococcusfaecalisCandidaalbicansEnterobactercloacaeStreptococcuspneumoniaeFlavobacteriumokeanokoitesBacillussubtilisHaemophilusinfluenzaeBacilluscereusProteusvulgarisArthrobacterluteusSalmonellatyphimuriumYersiniaenterocoliticaFrancisellaphilomiragiaTB337Promega:8008108133E-mail:[e-mail]337710b)BacTiter-GloTM4MHIIMuellerHintonBecton,DickinsonCompany,#297963FDANCCLSMHDMSO2%5%BacTiter-GloTMTurnerBiosystemsTD-20/20ATPATPATPATPBacTiter-GloTM1-5ATP96/ATPBacTiter-GloTMATPATP,ATP2TB337Promega:8008108133E-mail:[e-mail]337810c)BacTiter-GloTMBacTiter-GloTMBacTiter-GloTMSigma(LOPAC,#8,80)53LOPAC3LOPACD6,D11H75.BacTiter-GloTMATCC25923MuellerHinton(MHII)BD#297693IV37oCMHII100LOPAC50X96245ul5ul50X37oC5100ulBacTiter-GloTMIIITurnerBiosystemsVeritasTM#E65011-493-962%5-889-9232ug/mloxacillinpiperacillin9-8810uMLOPACBacTiter-Glo™oxacillin6OxacillinMHIIMICATCC25923Oxacillin0.125-0.5ug/mlBacTiter-Glo™IC75-IC90TB337Promega:8008108133E-mail:[e-mail]-Glo™ATCC25923oxacillin537oCNCCLS19oxacillinRLUTurnerBiosystemsVeritas™E6501BacTiter-Glo™O.D7BacTiter-Glo™O.D50.025BacTiter-Glo™6O.D7.BacTiter-Glo™ATCC25922MuellerHintonIIMHIIB.D.297963IV37oC50mlMHII1106250rpm37oCIIIBacTiter-Glo™Veritas™BeckmanDU650600nmO.D600RLUO.D1081TB337Promega:8008108133E-mail:[e-mail]3371010BacTiter-Glo™BacTiter-Glo™III.AIII.BIII.A1BacTiter-Glo™BacTiter-Glo™482BacTiter-Glo™3G8230G823110mlG8232G8233100mlBacTiter-Glo™BacTiter-Glo™/BacTiter-Glo™4BacTiter-Glo™1515ATPIII.B196100ul38425ul234BacTiter-Glo™96100ul100ul5560.25-1