丹参中丹酚酸B的提取分离及分析方法研究进展

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WorldScienceandTechnology/ModernizationofTraditionalChineseMedicineandMateriaMedicaB*(200237):2006205231:2006208230*:,,,,:,E2mail:whan@ecust.edu.cn:B,:B(Salviamiltior2rhizaBunge),,,B,,2%8%[1]B,,,,BBB,B,,,,CO21.[2],,:20,8115h,2,11.5h,26,110h[3]::75%,85%,,60%,,2311%,19.7%2.[4]30%942007Vol.9No.1WorldScienceandTechnology/ModernizationofTraditionalChineseMedicineandMateriaMedica,50%,70%,100%,30%,50%,70%,100%30mL,B,B70%;70%,70%98.8%,,70%95.06%,,B3.[5],A,,,:1050%2,1.5h,3[6],75%,BYWG-C18,-5%,1.0mL/min,281nmB0.171.70g(r=019997),98.9%,RSD=1.82%4.CO2[7]CO2,,,A20%,35%,2,,[8]CO2,,,5.[9]A,,,695%,320W30min,12,A,:[10],A,,,,12,60min,320W,1.2971.266.;[11]ABC7,-,B,-,[12]70,,,:703,112,210,2h:7.[13],,,,,,,,05WorldScienceandTechnology/ModernizationofTraditionalChineseMedicineandMateriaMedica24,,8.[12],,,,,,,,,,,,:,18mm,60,,B,ABC,,,,1980,[14],;,,,,;,,,0.001mol/L,,,(BB)[15],,,,,,,,,,,[16]SIPI905B,,:B8.72mg/g,420%B96.28%,251.82%B[17]F,B,(B)87.9mg/g,360%,-B93.7%,41%8%B,[18]B,B,B,B90.6%,91.8%,,[19],,,,,B[20],,--,,,,,B[21]BB,,,,B,B1.152007Vol.9No.1WorldScienceandTechnology/ModernizationofTraditionalChineseMedicineandMateriaMedica,,HPLC[11]7ABC,,62.[22]B-B12.23195168g/mL,99.26%,RSD1.8%,3.0g/mL3.B,[23]B:DiamonsilODS-C18,---36%,1.0mL/min,280nm,10L,B0.496.15g[4]B,HPLCAlltimaC18,-0.5%,,1.0mL/min,290nmB0.070.70g(r=0.9997)RSD1.62%,RSD3%B,,B[24]-9(BIA),0.05%-:025min281nm,555min254nm;;:0.8mL/min:95.62%104.22%,RSD1.42%3.52%[25]-B:BACKMANODS,-0.5%,1.0mL/min;EX=338nm,EM=443nm;5LB0.121.2g,(r=0.9998);98.6%108%;0.013g/mL,BB,B,B,,,B,B,B,1..,2005,2(2)146.2,,..,2002,21(6)46.3,..,2005,33(3)6.4,,,.BHPLC.,2004,1862.5,,,..,1999,21(8)386.6,,,.B.,2001,26(12)848.7,,.CO2.,2001,57.8,,,.CO2.(),2004,25(6)614.9,,,..,2005,22(3)243.10,,,.,2005,36(11)25WorldScienceandTechnology/ModernizationofTraditionalChineseMedicineandMateriaMedica16401641.11,..,1993,28(7)54354.12.-[].,2003.13.[].,2003.14,..,1981,16(9)2425.15,.(CN03132382.0).16,,,.B.,2003,26(6)339.17,,,..,2005,16(3)206.18,,,.B.,2005,16(6)475.19.:[].:,2002.20.[].,2002.21,.B(CN03151476.6).22,,,.B.,2004,24(4)439.23,,,.B.,2003,23(2)149.24WangHongandWangQiang.High-PerformanceLiquidChromato2graphicDeterminationofNineMajorConstituentsinRootsofSalvia.JournalofChinesePharmaceuticSciences,2002,11(4)148.25,,,.-B.,2005,23(5)817.ProgressReviewofExtraction,Purification,andAnalysisofSalvianolicAcidBSunJinlan,HanWei(ModernEngineeringCenterforTraditionalChineseMedicine,SchoolofPharmacy,EastChinaPolytechnicUniversity,Shanghai,200237)SalvianolicacidBisapopularelementusedinangiocardiopathymedicament.ThispaperpresentsabriefreviewofrecentprogressesmadeinextractionandpurificationofSalvianolicacidBinMiltiorrhizaBunge,andassociatedanaly2zingmethods.Keywords:SalviamiltiorrhizaBunge;SalvianolicacidB;extraction;purification;analyzingmethod(:,:,:)200620062007125,,,,2006245,234,807,6;25,2;48,2005,()35

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