invitrogen-lipofectamine2000

Forresearchuseonly.Notforhumanoranimaltherapeuticordiagnosticuse.Lipofectamine®2000ReagentCat.nos.11668-030Size:0.3mLStoreat4°C(donotfreeze)11668-0270.75mL11668-0191.5mL11668-50015mLPublicationPartNo.11668.2k.ppsMAN0000995Rev.Date12March2012Description• Lipofectamine® 2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids (DNA and RNA) into a wide range of eukaryotic cells.• Nucleic acid-Lipofectamine® 2000 complexes can be added directly to cells in culture medium, in the presence or absence of serum.• It is not necessary to remove complexes or change/add medium after transfection.ImportantGuidelinesforTransfection• Use Opti-MEM® I Reduced Serum Medium (Cat. no. 31985-062) to dilute Lipofectamine® 2000 Transfection Reagent and nucleic acids. • The amount of Lipofectamine® 2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by tesing the recommended four concentrations of Lipofectamine® 2000 Reagent to determine an optimum amount.• Prepare high-quality plasmid DNA at 0.5–5 µg/µLindeionizedwaterorTEbuffer.AGFP(greenfluorescentprotein)plasmidcanbeusedtodeterminetransfectionefficiency.• For additional information, and protocols on transfecting suspension cells refer to the manual at :RESEARCHUSEONLYThepurchaseofthisproductconveystothepurchaserthelimited,non-transferablerighttousethepurchasedamountoftheproductonlytoperforminternalresearchforthesolebenefitofthepurchaser.Norighttoresellthisproductoranyofitscomponentsisconveyedexpressly,byimplication,orbyestoppel.Thisproductisforinternalresearchpurposesonlyandisnotforuseincommercialapplicationsofanykind,including,withoutlimitation,qualitycontrolandcommercialservicessuchasreportingtheresultsofpurchaser’sactivitiesforafeeorotherformofconsideration.Forinformationonobtainingadditionalrights,[e-mail],LifeTechnologies,5791VanAllenWay,Carlsbad,California92008.LimitedProductWarrantyLifeTechnologiesCorporationand/oritsaffiliate(s)warranttheirproductsassetforthintheLifeTechnologies’GeneralTermsandConditionsofSalefoundonLifeTechnologies’websiteat(S)DISCLAIMALLWARRANTIESWITHRESPECTTOTHISDOCUMENT,EXPRESSEDORIMPLIED,INCLUDINGBUTNOTLIMITEDTOTHOSEOFMERCHANTABILITY,FITNESSFORAPARTICULARPURPOSE,ORNON-INFRINGEMENT.TOTHEEXTENTALLOWEDBYLAW,INNOEVENTSHALLLIFETECHNOLOGIESAND/ORITSAFFILIATE(S)BELIABLE,WHETHERINCONTRACT,TORT,WARRANTY,ORUNDERANYSTATUTEORONANYOTHERBASISFORSPECIAL,INCIDENTAL,INDIRECT,PUNITIVE,MULTIPLEORCONSEQUENTIALDAMAGESINCONNECTIONWITHORARISINGFROMTHISDOCUMENT,INCLUDINGBUTNOTLIMITEDTOTHEUSETHEREOF.©2012LifeTechnologiesCorporation.Allrightsreserved.ThetrademarksmentionedhereinarethepropertyofLifeTechnologiesCorporationortheirrespectiveowners.ScalingUporDownTransfectionsUse the multiplication factor in the following table to scale the volumes for your transfection experiment. The factor is based on the relative surface area of a single well from a 24-well plate.CultureVesselMultiplicationFactorCultureVesselMultiplicationFactor96-wellplate0.2X6-wellplate5X48-wellplate0.5X60mmdish11X24-wellplate1X100mmdish29X12-wellplate2XT75flask39XCo-TransfectionofPlasmidDNAandsiRNAPlasmid DNA and siRNA can be transfected at the same time using Lipofectamine® 2000 Reagent by adding 40 pmol (~0.6 µg) of siRNA per 1 µgofDNA.mRNATransfectionmRNA can be transfected in a 24-well plate by using Lipofectamine® 2000 Reagent by adding 0.5–1 µg of mRNA per well.Forresearchuseonly.Notforhumanoranimaltherapeuticordiagnosticuse.Lipofectamine®2000ReagentCat.nos.11668-030Size:0.3mLStoreat4°C(donotfreeze)11668-0270.75mL11668-0191.5mL11668-50015mLPublicationPartNo.11668.2k.ppsMAN0000995Rev.Date12March2012Description• Lipofectamine® 2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids (DNA and RNA) into a wide range of eukaryotic cells.• Nucleic acid-Lipofectamine® 2000 complexes can be added directly to cells in culture medium, in the presence or absence of serum.• It is not necessary to remove complexes or change/add medium after transfection.ImportantGuidelinesforTransfection• Use Opti-MEM® I Reduced Serum Medium (Cat. no. 31985-062) to dilute Lipofectamine® 2000 Transfection Reagent and nucleic acids. • The amount of Lipofectamine® 2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by tesing the recommended four concentrations of Lipofectamine® 2000 Reagent to determine an optimum amount.• Prepare high-quality plasmid DNA at 0.5–5 µg/µLindeionizedwaterorTEbuffer.AGFP(greenfluorescentprotein)plasmidcanbeusedtodeterminetransfectionefficiency.• For additional i