PageRuler-Prestained-Protein-Ladder-26616-Thermo-S

PRODUCTINFORMATIONThermoScientificPageRulerPrestainedProteinLadder#266162x250µlLot:XXXXXXXXExpiryDate:__WARNING!HARMFULIFINHALEDORABSORBEDTHROUGHSKIN.CAUSESRESPIRATORYTRACT,EYEANDSKINIRRITATION.MAYCAUSEALLERGICSKINREACTION.MAYBEHARMFULIFSWALLOWED.CONTAINSMATERIALWHICHCAUSESDAMAGETOTHEFOLLOWINGORGANS:KIDNEYS,LUNGS,UPPERRESPIRATORYTRACT,SKIN,EYES.CONTAINSMATERIALWHICHMAYCAUSEDAMAGETOTHEFOLLOWINGORGANS:LIVER,GASTROINTESTINALTRACT.Avoidexposure-obtainspecialinstructionsbeforeuse.Donotbreathevaporormist.Donotingest.Donotgetineyesoronskinorclothing.Useonlywithadequateventilation.Keepcontainertightlyclosedandsealeduntilreadyforuse.Washthoroughlyafterhandling.RefertoMSDS.|0026610®~0026616|00072559?~XXXXXXXXStoreat-20°C™PageRuler™PrestainedProteinLadderisaprestainedmixtureoftenrecombinantproteinsrangingfrom10kDato170kDa.Threedifferentchromophoresareboundtotheproteins,producingabrightlycoloredladder.Theproteinladderisconvenientlypackagedandreadytousewithnoheating,dilutingoradditionalreducingagentnecessary.Lot-to-lotvariationoftheapparentmolecularweightofprestainedproteinsis~5%.StorageBuffer:62.5mMTris•H3PO4(pH7.5at25°C),1mMEDTA,2%(w/v)SDS,10mMDTT,1mMNaN3,33%(v/v)glycerol.ImportantProductInformation•Donotboiltheproteinladder.•Theproteinladdercanbestoredat4ºCforupto3month.•ForpreciseproteinMWdeterminationusesthePageRulerBroadRangeUnstainedProteinLadder(#26630).•EachlotofthePageRulerPrestainedProteinLadderiscalibratedagainstPageRulerUnstainedProteinLadderandcalculatedapparentmolecularweightsarereportedinthelotspecificproductinformationsheetprovidedwiththeproduct.Rev.5MigrationPatternsofPageRulerPrestainedProteinLadderRecommendationsforLoading1.Thawtheladderatroomtemperatureforafewminutestodissolveprecipitatedsolids.Donotboil!2.Mixgently,butthoroughly,toensurethesolutionishomogeneous.3.LoadthefollowingvolumesoftheladderonanSDS-polyacrylamidegel:–5µlperwellforminigel,–10µlperwellforlargegel.UsethesamevolumesforWesternblotting.Theloadingvolumeslistedabovearerecommendedforgelswithathicknessof0.75-1.0mm.Theloadingvolumeshouldbedoubledfor1.5mmthickgels.ImportantNotes•PrestainedproteinscanhavedifferentmobilitiesinvariousSDS-PAGE-buffersystems.However,theyaresuitableforapproximatemolecularweightdeterminationwhencalibratedagainstunstainedstandardsinthesamesystem.Seethetableprovidedformigrationpatternsindifferentelectrophoresisconditions.•Inlow-percentagegels(10%),thelow-molecularweightproteinsintheladdermaymigratewiththedyefront.•PageRulerPrestainedProteinLaddercanbeusedinWesternblottingwithallcommonmembranes:PVDF,nylonandnitrocellulose.•LongertransfertimesorhighertransfervoltagesmayberequiredforWesternblottingoflarge(100kDa)proteins.RepresentativepictureofPageRulerPrestainedProteinLadder4-20%Tris-glycineSDS-PAGEGeneralReferencesBurnette,W.N.(1981).“Westernblotting”:electrophoretictransferofproteinsfromsodiumdodecylsulfate–polyacrylamidegelstounmodifiednitrocelluloseandradiographicdetectionwithantibodyandradioiodinatedproteinA.AnalBiochem112(2):195-203.Kurien,B.T.andScofield,R.H.(2003).Proteinblotting:areview.JImmMeth274:1-15.Laemmli,U.K.(1970).CleavageofstructuralproteinsduringtheassemblyoftheheadofbacteriophageT4.Nature227:680-5.Towbin,H.,etal.(1979).Electrophoretictransferofproteinsfrompolyacrylamidegelstonitrocellulosesheets:procedureandsomeapplications.ProcNatlAcadSciUSA76:4350-4.ThisproductismanufacturedunderthelicenseforStrep-tag®technologycoveredbyUSpatentsNos.5,506,121,6,103,493andforeigncounterparts.PRODUCTUSELIMITATIONThisproductisdeveloped,designedandsoldexclusivelyforresearchpurposesandinvitrouseonly.Theproductwasnottestedforuseindiagnosticsorfordrugdevelopment,norisitsuitableforadministrationtohumansoranimals.Pleasereferto©2013ThermoFisherScientific,Inc.Allrightsreserved.AlltrademarksarethepropertyofThermoFisherScientific,Inc.anditssubsidiaries.