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!050016.78A1000-5854200401-0082-04E.coli...!1.1promotermRNA.lactrp!pLtrplacUV5tac.2-355/TTGACA-105/TATAAT1.2-35-1017171.terminatorDNAmRNA.UAA1UUAAU2.1.2mRNAAUGSDUAAGGAGGAAGGAmRNA3.1SDUAAGGAGGAAGGA362mRNA4SDAUG933SDAT.5AUG!200306243001621979.28120041Journalof~ebeiNormalUniversityNaturalScienceeditionVol.28No.1Jan.2004.AUGSD.RBSmRNA5/mRNAmRNA30s6RBSATmRNA5/PCRmRNA5/2SD.1.3mRNAmRNAmRNA.mRNA2.pBR322CoIEI.2CoIEI108RNARNAIROP.ROPRNAI...par.!1!2tRNAbiasedcodonsrarecodons.ArgAGAAGGProCCCCCUCCA..2.1tRNAtRNAtRNA.tRNAtRNAArgAGGAGABrinkmann7ArgArgtRNAAGGAGAdnaYtRNA5%30%TV!5.34%AGGAGAtRNAArgAGGAGA8.9.2.2.Makoff10C3%!4%11%!14%.NPCRN7!85/G+C40%11.381mRNA3.!mRNAmRNAmRNA.3.1mRNAmRNA.mRNAompAmRNA5/mRNA5/mRNA.3/mRNAmRNA.repetiveextragenicpalindronicREPmRNA3/!5/REPDNAmRNA12.3.2..12345.mRNA..4.1pETT7RNABL21DE3JM109DE3HGMS174DE3.BL21trxBAD49413.4.2inclusionbody.37C.pET25C30C15!20C.4.3OD600OD6000.6!0.84828.14ACCBL1E3O6.1..3.4.5.6.7.8.91.IPTG!DO6.6.4.4.pETBIPTG6%15...1.M.199.138-14..M..1.117-119.3C~EN~YBIERKNESRKUMARRetal.eterminationoftheoptimalalignedspacingbetweentheSseCuencesandthetranslationinitiationcodonofE.colimRNAsJ.NucleicAcidsRes19944953-4957.4GOLL.Enchancedtranslationalefficiencywithtwo-cistronexpressionsystemJ.MethodsinEnzymology19918511-17.5.BM.1987.61-68.6ESMIT~M~UINJvan.ControloftranslationbymRNAsecondarystructureinE.coliJ.MolBiol199444144-15.7BRINKMANNUMATTESREBUCKELP.OptimizingtheexpressioninE.coliofasyntheticgeneJ.Gene19898519.8ANREWSBAARIG~ANNINGetal.AtightlyregulatedhighlevelexpressionvectorthatutilizesthermosensitivelacpromoterProductionofthehumanTcellreceptorV!5.3inE.coliJ.Gene1996181-11-159.9~U~KRC~OE~LEESOEetal.~ighlevelexpressionofhumanlipocortinannexininE.coliJ.BiotechNolLett199618163-168.1MAKOFFAJOXERM.ExpressionidentificationandcharacterizationofrecombinantgeneproductsinE.coliJ.NucleicAcidsRes19891741191.11JW.M..8-844.1.M..193-194.13NOVAGEN.pETSystemManualM.1thed.s.l.s.n..14.BL1E3J.1361-4.15.pETJ.5189-93.ExpressionalOptimizationofheterologousgeneinE.coliCAO~ong@IUYanWANGGangCollegeofLifeScience~ebeiNormalUniversity~ebeiShijiazhuang516ChinaAbstractE.colihaswidelybeenusedinexpressingagreatvarietyofheterologeousgenesbutmanyfactorscontributetoinfluencingtheexpression.Thisreviewsummarizestheoptimarizedexpressionstrate-giesaccordingtotheinfluencialfactorswhichwillhelptotakeeffectivemeasurestoimprovetheexpres-sionalefficiencyofheterologousgenesinE.coli.KeywordsE.coliheterologousgeneoptimarizedexpression581