LSM880

ZEISSLSM880withAiryscanRevolutionizeYourConfocalImagingProductInformationVersion1.1ConfocalAiryscanClickheretoviewthisvideo2Yoursamplestendeithertobeverysmall,moveveryfastorbleachveryquickly.Ordoallofthatatonce.Togetunbiaseddatafromlivecellsorotherweaklylabelledsamples,there'snosuchthingastoomuchsensitivity,resolutionorspeed.Eachphotonofemissionlightisprecious.Nowyoucanusemulticolorsampleswithanylabelandgetimagequalitylikeyou’veneverseenbefore.WithAiryscanyouarealwaysabletoselecttheoptimalacquisitionstrategyforyoursample:Simplydecidewhetheryouwanttogain1.7xhigherresolutioninallthreedimensions–resultingina5xsmallerconfocalvolume.Orpushthesensitivitybeyondthelimitsofallconventionalconfocals.Orusetheincreaseinsignal-to-noiseratiotospeedupyourimageacquisition.Thechoiceisyours.RevolutionizeYourConfocalImagingwithAiryscanSeeforyourselfhowAiryscangivesyoubetterdatathaneverbefore.Bookahands-ondemon-strationinoneofourZEISSMicroscopyLabsnow.(green),AdapterProteinAP-3(magenta)andSeptinA(red).CourtesyofS.Traikov,BIOTEC,TUDresden,Germany›InBrief›TheAdvantages›TheApplications›TheSystem›TechnologyandDetails›ServiceAiryscanAiryscanConfocalConfocalClickheretoviewthisvideo3Airyscan:EnteraNewWorldofConfocalPerformanceImagineatrueconfocalthatcouldgiveyouhighsensitivity,improvedresolutioninx,yandz,andhighspeed–allthatinasinglesystem.WithAiryscanyouwillbeincreasingtheresolutionofyourimagingfarbeyondtheresolutionofaclassicconfocalpointscanningmicroscope.Youcanresolve140nmlaterallyand400nmaxially,at488nm–withoutsacrificingsensitivityorspeed.IntheoptionalVirtualPinholeMode,youcande-cideevenaftertheacquisition,whichpinholesizebestsuitsyourapplication.PerformQuantitativeImagingScientificresultsdependonunbiaseddata.LSM880guaranteesgentleimagingofyoursamplethroughhomogeneousillumination,combininglinearscanningwithasensitivedetectioninfra-structure.Workingwithidenticalpixeltimesandcontinuousscannermonitoring,youcanperformquantitativeimagingatallspeedsandscanmodes.Getrobustandreliableresults,evenfromyourmostdemandingsinglemoleculeimagingandanalysis.IncreaseYourProductivitySavetimeoninvestigationsintolocalizationandinteractionofproteinsthatrequiremultiplefluores-centlabels.LSM880collectsallthesesignalsinonego,withhighspeedandhighsensitivity.Youperformsimultaneousspectraldetectioninasinglescanwiththehighestnumberofdescannedornon-descannedchannels–featuringGaAsPtechnology,too.LSM880letsyoutakefulladvantageoflargefieldsofviewandthehighestspeedofanylinearscanningconfocal–upto13fps.Simpler.MoreIntelligent.MoreIntegrated.Heterochromatinprotein1(HP-1)fusedtoGFPandexpressedinthenucleusofahumanHepG2cell.LeftpanelshowsthedistributionofHP-1betweenEuchromatinanddenserHeterochromatinareas.RightpanelrepresentsabrightnessmapdemonstratingdimerizationofHP-1withinheterochomaticregions.Sample:courtesyofP.Hemmerich,Leibniz-InstituteforAgeResearch(FLI),Jena,GermanyMonolayerofhumanintestinalstemcells.F-actin(phalloidin,green),alpha-fodrin(red).CourtesyofO.Kovbasnjuk,JohnsHopkinsUniversitySchoolofMedicine,Baltimore,USAMitochondria,RK13TOMM20labeledwithCerulean3;comparingconfocalGaAsPandAiryscandetection.Sample:courtesyofM.Davidson,TheFloridaStateUniversity,Tallahassee,USA›InBrief›TheAdvantages›TheApplications›TheSystem›TechnologyandDetails›ServiceResolutionConfocalAiryscanSpeedSNRResolutionandSNR=oversamplingandlongerpixeldwelltimes(increaseimageformatanddecreasespeed)Speed=undersamplingandshorterpixeldwelltimes(keepimageformatandincreasespeed)SNR=undersamplingandlongerpixeldwelltimes(keepimageformatanddecreasespeed)Resolution=oversamplingandshorterpixeldwelltimes(increaseimageformatandkeepspeed)Sensitivity(SNR)24.0%ResolutioninZ24.7%ResolutioninXY15.1%Other11.0%MaximumFrameRate25.3%Airyscan–higherResolutionAiryscan–higherSNRAiryscan–higherSpeed0.5µm5µm0.5µmConfocalConfocal5µmConfocalClickheretoviewthisvideoClickheretoviewthisvideo4YourInsightintotheTechnologyBehindItAsurveyamong250researchersworkingwithconfocalmicroscopesrevealedthattheirimagingwouldbenefitmostfromanincreaseinsensitivity,resolutionandspeed.Airyscanextendsexactlythoseparametersforyourexperimentsandevenallowscombinations,suchasincreasedresolutionandsignal-to-noise-ratio(SNR)atagivenspeed.›InBrief›TheAdvantages›TheApplications›TheSystem›TechnologyandDetails›ServiceHEp2cells,movementofGFPlabelledkinetochores.CourtesyofV.Döring,Leibniz-InstituteforAgeResearch(FLI),Jena,Germany10µmConfocalAiryscan4.Airydisk5.Airyscandetector1.Mirror2.Emissionfilters3.Zoomoptics123454Clickheretoviewthisvideo5YourInsightintotheTechnologyBehindItTheAiryscanPrincipleThebeampathofeverymicroscopetransformstheemissionsignalofevenaninfinitesimallysmallspotintoacomplexthree-dimensionalstructurecalledanAirydiskorAirypattern.Thistransformationisknownaspointspreadfunction(PSF).Aclassicconfocalmicroscopeilluminatesonespotonyoursampletodetecttheemittedfluorescencesignal.Out-of-focusemissionlightisrejectedatapinhole,thesizeofwhichdetermineshowmuchoftheAirydiskreachesthedetector.Youcanin