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ViiA™7 PCR212 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.312 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.ABReal‐Time PCR 1996Introduced ABI Prism®7700 Instruments1998Launched GeneAmp ®5700 Instruments2000Launched ABI Prism®7900 Instruments2003Launched ABI Prism®7000 Instruments2005Launched Applied Biosystems 7300/7500 7500FInstruments2007Launched StepOne™and StepOnePlus™Instruments412 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.AB7启PCR●35384PCR●●OptiFlex™21●9696384TaqMan®4512 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.ViiA™7 Real‐Time PCR System“Redefining the Market –Unsurpassed Productivity & Convenience”•••TaqManArray60%•Block••ReadiApp™•612 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.●ReadiApp™●TaqMan®Assay●712 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.‐●Name the instrument and create an Avatar●Create shortcuts to common protocols●On‐board timer to indicate time to completion812 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.灵OptiFlex™System●灵−384‐wells 1uL(TaqMan®Array Cards)−1.5 异−: 异异●Multiplex−66达21−TaqMan®SYBR®912 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.4Block●Block1−(96 vs. 384)−(96 vs. 384 vs. TAC)●384‐BlockTaqManBlock●96‐Block96‐Block1uL1uLTAC1‐30uL5‐20uL384ProgrammableValidated1‐100uL5‐30uLFast 961‐200uL10‐100uL96ProgrammableValidated1012 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.1112 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.Down to 1.5 fold change for single plex reactionUp to 9 logsDown to 1 copy~35 min for 384‐well3.0°C/sec (384‐well)+/‐0.25°C1212 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.灵: 1.5 异RNaseP copies:‐10,000‐6,667‐4,500‐3,000‐1,500‐1,000TaqMan Gene Expression MMx, 5ul Rxn, 384‐well format, Standard Run conditions1312 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential., (384‐well)Amplification of plasmid DNA (7‐7E10 total copies/rxn)35达9.TaqMan Fast Universal MMx, 5ul Rxn, 384‐well format1412 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.MultiplexMultiplex on cDNA with 5 probes + passive reference.Proprietary dyes and master mix in development.1512 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.极: 1 CopyDigital PCR (PCR): Raji gDNA1.05. 71% (260/368) PCR.:1.05 copy per well will result in ~70% positive reactions. Single molecule detection is possible following Poisson distribution.1.0570%384‐(368 +16 NTC). , 5ul, Fast Universal MMx).1612 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.Ct384‐wellAmplification of the RNase P Gene showing 144 replicates of 5,000 and 10,000 copy populations on a 384‐well block using fast run conditions1712 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.CtTaqMan Array CardsCt Standard Deviation of 0.092Amplification of the TGF‐Beta Gene on a TaqMan Array Card witha Full‐Plate of replicates.Gene Expression Master Mix, standard run conditions1812 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.Block●异1-23-45-67-89-1011-1213-1415-1617-1819-2021-2223-24O-PK-LG-HC-D94.0094.2594.5094.7595.0095.2595.5095.7596.00ViiA?7TaqMan?ArrayBlockThermalUniformity@90C1912 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential., Blocks异Ct SDAve CtViiA™7 BlockTGF‐Beta FAM18sVICTGF‐Beta FAM18sVICA384w0.0520.02727.1712.38A384w0.0570.03727.1312.38A384w0.0540.02827.1612.37B384w0.0610.03227.2512.24B384w0.0530.02827.2412.24B384w0.0530.02827.2412.23ATAC0.0880.13727.1112.39ATAC0.0840.13027.1512.42ATAC0.0800.14027.1412.41BTAC0.0870.08427.2412.30BTAC0.0950.09027.2212.27BTAC0.0910.09827.2112.282012 April 2010©2010 Life Technologies. All Rights Reserved. | Proprietary and Confidential.*2009 Demo Sites and Usability TestingDemo Sites will generate over 10 application notes & Your Innovative Research (YIR) in key areas: Multiplexing, Genotyping, ViiA™7 vs. 7900HT and Roche LC480“…The Next Generation Instrument in the format of a low‐density array/microfluidic card …are in agreement with results obtained on ABI 7900HT device in a 96‐well format.”—Principal Investigator, Medical University of Vienna“Now I can go home early on Friday”‐‐Senior Scientist, Genentech“What I really appreciated about the instrument is the immediate easy‐to‐use feeling I had when playing around with the software…and also the comfort with the instrument itself.”‐Laboratorio Patologia Molecolare2112 April 2010©2010 Life Technologies. All Ri