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29920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．29No．9Sep．2011SD1211111．1000292．450002SD。SDGLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACCOR、T3、T4、TSH、E2、T。GLU、COR、TP、ALB、GOT、GPT、UA、CK、CKMB、LDH、LAC。SD。R332A1673－7717201109－1984－03ComparisonofTwoBloodCollectionMethodsonBiochemistryandEndocrineinMaleSDRatsLILian-zhen12LIUXiao-qing1ZHANGQian1LINZhi-jian1ZHANGBing11．BeijingUniversityofChineseMedicineBeijing100029China2．HenanAgriculturalUniversityZhengzhou45002HenanChinaAbstractObjectiveTocomparetheeffectsoftwobloodcollectionmethodsanestheticanddecapitatedonbiochemis-tryandendocrineinmaleSDrats．MethodsMaleSDratsweredividedintotwogroups．Bloodsampleswerecollectedaf-teranesthetizedbyintraperitonealinjectionofpentobarbitalsodiumanddecapitationrespectively．SerumparametersofGLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACweredetectedbyautomaticbiochemicalana-lyzer．TheendocrinesystemindexofserumE2TCORT3T4TSHweredeterminatebyradio－immunity．ResultsSerumGLUCORTPALBGOTGPTUACKCKMBLDHLAClevelsweredifferencebetweenthesetwobloodcollectionmethods．ConclusionTherearesignificantinfluencesonbiochemistryandendocrineparametersbetweenthesetwobloodcollectionmethods．Itshouldpaymoreattentionontheeffectsofbloodcollectionmethodinanimalexperimentandchooserationalbloodcollectionmethodwiththeexperimentalrequirements．KeywordsRatPentobarbitalsodiumDecollationBloodbiochemistryEndocrinehormone2011－04－029732007CB5126051979－。1959－、。SD。。。。。1－23、、、。11．1SD20240～250gSCXK2007－0001。SPF。1．2Sigma200704152%。GLU80125L041TG71129K021TC911171ITP003051AALB001201AALT71114J811AST80125L041CRE912141KABUN489129920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．29No．9Sep．201180313B811UA80213A811LDH80318C011LAC80318C011CK9020911ACKMB71116K211。。1．3JOUANxh6080CX4。1．410。3312h2%45mg/kg。SD。1～2h3000r/min15min。1．5GLU、TG、TC、TP、ALB、GOT、GPT、CRE、BUN、UA、CK、CKMB、LDH、LACCX4GLUTGTCTPALBGOTGPTIFCCCREBUNUVUA－PODCKCKMBDGKCLDHLAC。COR、T3、T4、TSH、E2、T。1．6SAS8．0珋x±stP＜0．05。22．11GLUP＜0．01TPALBUAP＜0．01TGTC。1n=10珋x±sGLUmmol/LTGmmol/LTCmmol/LTPg/LALBg/LUAμmol/L5．02±0．970．60±0．130．84±0．1764．35±2．8027．19±1．4957．37±14．753．57±0．800．89±0．451．02±0．2673．32±2．4730．31±1．2090．23±26．08P＜0．01。2．22n=10珋x±sGOTU/LGPTU/LBUNμmol/LCREμmol/L177．70±33．1773．40±10．084．98±0．44125．32±46．19290．10±55．87113．10±24．344．99±0．84138．85±47．17P＜0．01。2GOTGPTP＜0．01BUNCRE。2．33LAC、LDH、CKCKMBP＜0．01。3n=10珋x±sLDHU/LLACumol/LCKU/LCKMBU/L2060．30±406．876．61±0．83561．80±149．771078．10±287．062478．90±321．668．59±1．192491．89±266．536893．67±669．92P＜0．01。4n=10珋x±sCORng/mLE2pg/mLTng/mLT4ng/mLT3ng/mLTSHuIU/mL10．84±5．8033．74±12．025．74±4．2478．51±17．970．56±0．132．83±0．385．56±3．4626．19±19．062．83±1．8191．59±18．370．58±0．192．59±0．50P＜0．052．44CORP＜0．05E2、T、T3、T4、TSH。3。。、、、、、、。。SD。3．1。589129920119CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．29No．9Sep．20114－7、、。、、GLU、TP、ALBUATGTCGOTGPTBUNCRELAC、LDH、CKCKMB。。3．2－－、－－－－。COR、E2、T、T3、T4、TSH。8－9。CORE2、T、T3、T4、TSH。3．3。①、10。。11SD、。。②12。③、。、、GLU、TP、ALB、UA、GOT、GPT、LAC、LDH、CK、CKMB、COR。。1．J．200724119－20．2．SDJ．2008272299－3023．、、SDJ．2007178496－498．4．J．200616265－68．5．J．200616269－72．6．J．200616273－77．7．J．200616278－80．8．J．1997185474—476．9．、J．200577763．10．J．200919851－54．11．SDJ．200818143－45．12BalcombeJPBarnardNDSanduskyC．LaboratoryroutinescauseanimalstressJ．LabAnimSci2004436檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪檪42－51．“”、。。。“”。。。。、。500。“”。。。6891