QPCR-扩增效率

PCR41.11-18CTCTPCRCTCTCTPCR4CT1.1.3qPCRCTPCRqPCR•R20.980r|–0.990|•90-105%•qPCRPCRDNADNAcDNAlogCTPearsonrR2qPCR1.2A2n=nCT10DNA2n=10n3.32CT3.321.2BrPCR6EE=10–1/PCRPCR222210–1/–3.32E%=(E–1)x100%%=(2–1)x100%=100%1.2E=10–(1/–3.436)=1.954%=(1.954–1)x100%=95.4%1.9595.4%100%90–105%100%CTCT90%105%2.32.4PCR5rR2CTrR2qPCRr0.990R20.9801.2.iCycleriQ®PCR10A.B.CTlogr95.4%AB