浅谈使用分光光度计易出现的几个问题

FujianAnalysis&Testing2007,16(4)(,610083):,,,,:;;:TH744.12+1:B:1009-8143(2007)04-0050-03TheFrequencyoftheMattersisHigherOccurredonOperatingSpectrophotometerLiuXin,WangYuqiong,SongQingxiong(DepartmentofpublichealthofChengdumedicalcollege,Chengdu,Sichuan610083)Abstract:Thispaperistosumupthefrequencyofthematterswhichishigheroccurredonoperatingspectrophotometerinthestudents,toanalyzethepossiblecausestoproducethemattersandtodrawoffthecorrespondmethodstointendtoassistthestudentswelldealingwiththesimilarthemattersandimprovemoreaccurateexperimentalresultandmorehighexperi2mentalefficiency.Keywords:Spectrophotometer;cuvette;experimentalteaching1,,360nm-800nm,,,,,22.12.1.1,,0%,,100%,,721,117211.2.3.4.5.6.7.8.9.10.11.12.:2007-3-7:(1979),,,E-mail:[e-mail].052007,16(4):(1)(2),,(7),90,(6),,(4),(4),(3)(),,,,(4),(8),(9),(10),(11),(12),,,(11)[1],(6)(10),(10),0,,0,,100%2.22.2.1,,,,-:A=KLC,A(ABS);K;L;C,-:A=-lgT%=-lg(It.I0)=KLC,T%=It.I0100%,I0,ItK,,,,,2.32.3.1,,,,,,:(1)(2),(3),,,:(1),,,:,,:220nm700nm,(340nm):700nm,100%,,,0.5%,[2](2),:,,,,,::1:4:330:1:130,,,,3/4(),,,152007,16(3),,,,,22(3),,;,,,,;,,,2.4(1),,,;,;,(2),,[3]3,,,,,,,,,,(),,,[1].721-100[J].2006,l5(3):51-52[2],.[J].2006,11:211[3].721[J].,2004,6(22):25-2625