山楂黄酮类成分的分析测定_刘霞

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吉林农业大学学报　1997,19(3):47～49JournalofJilinAgriculturalUniversity刘　霞　赵淑春　程　宏　戴宝合(吉林农业大学农商学院　长春130118)　　采用HPLC法,分析测定了山楂原种(CrataeguspinnatifidaBge.)的8个单株,山里红(C.pinnatifidaBge.var.majorN.E.Br.)及伏山楂(C.praecoxDai&Fu.)果实与叶中黄酮单体化合物及总黄酮的含量。结果表明,山楂果实与叶中所含有的黄酮单体化合物种类及总黄酮含量有所不同;叶中总黄酮含量高于果实,山楂原种的总黄酮含量最高,其次是山里红,伏山楂最低。　山楂　黄酮类　HPLC法　S661.5AnalysisandDeterminationofFlavonoidsinHawthornsLiuXiaetal.(CollegeofAgri.andTrade,JilinAgri.Univ.,Changchun130118)Abstract　Theresultsshowedthatthekindsandthetotalcontentsofflavonoidsinfruitsandleavesofhawthornsaredifferent.Thetotalcontentsofflavonoidsinleavesishigherthanthatinfruits.ThetotalcontentsofflavonoidsinC.pinnatifi-daBge.ishighestandC.praecoxDai.&Fu.islowest.KeyWords:hawthorn;flovonoids;HPLC,。,50、,50,[1],,[2],。,,,[3～6]。(CrataeguspinnatifidaBge.)8、(C.pinnatifidaBge.var.majorN.E.Br.)(C.praecoxDai.&Fu.),。　130052:1997-05-06　,,32,,,DOI:10.13327/j.jjlau.1997.03.0091　1.1　。、、,。1.2　1.2.1　标准液及样品液的制备　10mg,20mL,1mL,25mL,。1g,24～48h,,10mL,。1.2.2　标准曲线的绘制及总黄酮含量的测定　1,2,3,4,5mL,,AlCl30.5mL,30min(276±2)nm。,(A),。1mL,。。1.2.3　黄酮单体化合物含量的测定　HPLC,2mL,2mg/mL。HPLC:C12(250mm×4mm);1():MeOH/(40∶60),0.8mL/min;2():MeOH/(60∶40),0.9mL/min。UV270nm;AuFs0.08,0.25mm/min。2　1。1　(%)Table1.Contentofflavonoidsinhawthorns(%)PartSpeciesComponentofflavonoidsCatechinVitexinTectoridinRutinQuercetinTotalcontentofflavonoids　FruitsCrataeguspinnatifidaBge.123456780.00632.68180.20550.43270.14670.05820.24240.02470.0050--0.0017----0.0048-0.01010.0037-0.00620.00950.01180.35460.25740.02480.03730.10920.12820.3097-0.0015------0.00280.83910.90970.57490.85490.66840.70060.75200.8068C.pinnatifidaBge.var.majorN.E.Br.0.0216-0.00280.1817-0.7257C.praecoxDai.&Fu.0.0871-0.02630.1998-0.645148　　　　　　　　　　　　　　　　　　　　　　19971PartSpeciesComponentofflavonoidsCatechinVitexinTectoridinRutinQuercetinTotalcontentofflavonoidsLeavesCrataeguspinnatifidaBge.123456780.0093-------0.02840.02650.03830.13670.09100.03490.04970.0570-0.0152----0.01250.00390.04250.00660.00790.01410.02200.02640.02880.02890.00270.00110.00370.00390.00450.00290.00080.00161.24161.07370.87641.34721.18461.31220.80850.9320C.pinnatifidaBge.var.majorN.E.Br.0.01030.00860.01000.14830.00130.9446C.praecoxDai.&Fu.0.14540.1454-0.0169-0.9566,5,、、、。;;。;,,,。3　　,,,。,。,,。,,,。　　　1　..,1987,22(10):46～552　..,1987,(3～4):13～153　..,1981,23(5):383～3874　..,1983,(2):18～235　..,1985,14(2):496　..,1987,18(10):97　　..,1989,11(2):38～394919　3　　　　　　　　　　　: