氨茶碱在家兔中的血药浓度测定--紫外分光光度法

(,434000)(,434000)(,434000)[]目的:建立氨茶碱的血药浓度测定方法方法:用紫外分光光度法测定氨茶碱血药浓度,测定波长为269nm结果:样品浓度在05~30g/ml范围内,与A样品空白呈良好线型关系,标准曲线:A=00416C+0002,r=09998;平均回收率8769%249%,精密度日内日间相对标准偏差均在5%以内结论:紫外分光光度法能消除血清干扰,方法简便快速准确[]氨茶碱;紫外分光光度法;血药浓度[]R9652;R9743[]A[]10089977(2004)03008002SpectrophotometryAnalysisofTheophyllamineinRabbitsSerumHuangXianju,GaoLin,MaLin(YangtzeUniversity,Jingzhou434000,China)[Abstract]Objective:ToestablishamethodofdeterminingtheconcentrationoftheophylineinrabbitsserumMethod:TheconcentrationoftheophyllamineinrabbitsserumwasdeterminedwithspectrophotometryThedeterminationwavelenghthwere269nmResults:Themethodwaslinearbetweentheophyllamineconcentrationof05~25g/ulandAThestandardregessivecurvewasA(g/ml)=00416C+0002,r=09998Theaveragerecoveryratewas(8769249)%,RSDinonedayorduringtwodayswasbelow5%Conclusion:Theresultsshowedthatthismethodcouldeliminatetheinterferenceofserumitselfandissimpleandrapidandaccurate[Keywords]Theophyline;Spectrophotometry;Concentrationinrabbitsserum,,,,,[1~4],,,1材料11optizen2120uv/(,)XW80A()800()(025g/2mL,031010,H146020149,)AR12,2kg,2方法与结果2101mol/L10ml,05g/ul8020049173JournalofHubeiMedicalStaffCollegeSep2004Vol17No3[]20040728[](1972),,,,222ml,,05ml,01mol/L02ml,:(95:5)5ml,,(2500r/min)10min,()40ml,01mol/L40ml,,(2500rpm)10min,()3~35ml,2315g/ul05ml,,,,269nm,,,,24241标准曲线制备50100150200300500l10ml,01mol/L10ml,33ml01mol/L10ml,A269,:A=00499C,r=09999,25~25g/ml242茶碱在血清中标准曲线的制备50100150200250300l05ml,051015202530g/ml,22,01mol/L4ml2ml,269,A,:A=00416C+0002,r=09998,05~30g/ml243提取回收率测试,051015202530g/ml,6,A,C,,,11(n=6)(g/ml)(g/ml)(%)(%)RSD(%)0504298857887662495891008963896332215135149009413201800390024062521476859035830253618454390244精密度测试051020g/ml,22,5,5,,22(n=5)(g/ml)xRSD(%)xRSD(%)050482343504326495100966136409238438201845242217984369245茶碱血药浓度的结果计算A,C(g/ml),C10C1(g/ml)3讨论TDXHLPC,,,,,,,,,,,,,,273nm274nm,269nm,,(下转第83页)81173:RSD=126%11(g)()%12340242902568024260252602438025010240502510100497499199428,,,020104011104011227011118,,[2]()22(,%)(n=5)(n=4)020104997101101110496898201122797798801111810051025AB(A)(B)HPLC3讨论-,-(73:27),,,LambdaII,,210~300nm,264nm,264nm30~60ug/ml,,(),,,,,,,,[][1]2:,2000:32[2]:,1992:439[](上接第81页)[][1](),1995,11(4):256[2],,,1999,18(6):5[3],,1998,11(1):30[4],,,2002,11(3):171[]83173: