Luminex液相芯片的发展及应用_谢冲

()FudanUnivJMedSci2010Mar,37(2)CSC-UC(2008610103)vCorrespondingauthorE-mail:[e-mail]谢冲(综述)王国民v(审校)(200032)=2090,LuminexLuminex100Luminex200Flexmap3DLuminex=Luminex;;Flexmap3D=Q78=ADevelopmentandapplicationofLuminexliquichipXIEChong,WANGGuo-minv(DepartmentofUrology,ZhongshanHospital,FudanUniversity,Shanghai200032,China)=AbstractLiquichipisanewtypeofbiochipemerginginthe1990s,whichisintegratedflowcytometry,lasertechnology,digitalsignalprocessingandtraditionalchemicaltechnologywiththegreatestfeaturesofhigh-throughputandhigh-flexibility.LuminexhaspromotednewproductsfromLuminex100/200toFlexmap3D.Thisisanintroductiontothedevelopment,principle,andapplicationofLuminexliquichip.=KeywordsLuminex;liquichip;Flexmap3D2090,[1-4],,Luminex,,Luminex2090,,xMAPxMAP,Luminex19991Luminex100Luminex100,1100,30min96Luminex100,Luminex2005Luminex200Luminex200Luminex100,FDALuminex100,Luminex200:,LuminexXY,Luminex,,Luminex2007Flexmap3D,xMAPFlexmap3DLuminex100/200,Flexmap3D,500,384LuminexLuminex,5.6Lm4:MicroPlexSeroMapxTAGMagPlex4,3MicroPlexSeroMap,xTAGMicroPlextag,MagPlex,(magneticbeads),Luminex2007,241()20103,37(2),,,(),,,,[5],Luminex100/2002,100[6]Flexmap3D3,5003,3(1),5003,Flexmap3D(2)1Flexmap3DFig1PrincipleofFlexmap3Dmicrobeadsdetection2Luminex100/200Flexmap3DFig2Comparisonofmicrobead-mapbetweenLuminex100/200andFlexmap3DA:Luminex100/200;B:Flexmap3D,,(),(3),,,;,[7]3LuminexFig3PrincipleofLuminexliquichipLuminexLuminex:(1)Luminex,,;(2),,,,;(3),Luminex(ELISA),(1)[8-9]1Flexmap3DTab1ComparisonofsensitivitybetweenFlexmap3DandotherlaboratorymethodsMethodsSensitivity(mol/well)ITA10-5~10-6ELISA10-8~10-9RIA10-10~10-12CLIA10-15~10-16TRFIA10-18~10-19Luminex100/20010-30Flexmap3D10-31~10-32Flexmap3DxMAP,xMAP,Flexmap3D,Flexmap3D3500,Luminex100/2005,,,Flexmap242,.Luminex3D384,,1h48000,(2),Luminex100/200,Flexmap3D(4),Flexmap3DxPONENT4.0,,Flexmap3D2Flexmap3DLuminex100/200Tab2ComparisonofFlexmap3DandLuminex100/200MethodsTest(50LL/well)Readtime(min)Tests(time/h)Luminex100/20096well1832000384well7530700Flexmap3D96well4564000384well135850004Flexmap3DLuminex100/200Fig4ComparisonofdynamicsbetweenFlexmap3DandLuminex100/200Luminex,,[10],,[11]Kricka[12]80%,Waterboer[10]59%,LuminexSeroMapSeroMap,Tim[10](PVA)(PVP)ChemiconCBS-KPVAPVP,,CBS-K,,1/3,PVAPVPCBS-K,[10],Luminex,:,Luminex,;PCR,Luminex,,Luminex,PCRLuminex免疫分析,ELISAOpalka[13](humanpapillomavirus,HPV)61116184,3pg/mWong[14]ELISAE(rWNV-E)IgGIgM,ELISA,,Luminex,Lachmann[15](HLA)(DSA);Brian[16]Luminex(HLA)(CDC),Luminex,[16],Vrzalova[17]ÒArellano[18]IL-8IL-1bNolen[19]55,,Flexmap3D,Flexmap3D激素水平测定Bellisario[20],T4(TSH),,17(17-OHP)(PAH)-6-,核酸检测LuminexEMSEDC,tagant-itag,,DNA,Smith[21]HIVHCVHSVBortolin[7],(PCR-SSCP),PCR-SSCP,243()20103,37(2),,20,,5~10,ELISA,,,[1]BayJT,GarredP.Rapidbead-basedimmunoassayformeasurementofmannose-bindinglectin[J].ScandJImmunol,2009,69(6):570-575.[2]Arellano-GarciaME,HuS,WangJ,etal.Multiplexedimmunobead-basedassayfordetectionoforalcancerproteinbiomarkersinsaliva[J].OralDis,2008,14(8):705-712.[3]LivingstonAD,CampbellCJ,WagnerEK,etal.Biochipsensorsfortherapidandsensitivedetectionviraldisease[J].GenomeBiol,2005,6(6):112-115.[4]LinkovF,YurkovetskyZ,LokshinA.Hormonesasbiomarkers:practicalguidetoutilizingLuminextechnologiesforbiomarkerresearch[J].MethodsMolBiol,2009,520:129-141.[5]OliverKG,KettmanJR,FultonRJ.MultiplexedanalysisofhumancytokinesbyuseoftheFlowMetrixsystem[J].ClinChem,1998,44(9):2057-2060.[6]SunK,WangQ,HuangXH,etal.Establishmentofmultiplexedmicrosphere-basedflowcytometricassayformultiplehumantumormarkers[J].ActaPharmSin,2007,28(12):2011-2018.[7]BortolinS,BlackM,ModiH,etal.Analyticalvalidationofthetag-ithigh-throughputmicrosphere-baseduniversalarraygenotypingplatform:applicationtothemultiplexdetectionofapanelofthrombophilia-associatedsingle-nucleotidepolymorphisms[J].ClinChem,2004,50:2028-2036.[8]RajeshK,AnnaT,ChalamKV.Multipleximmunoassayanalysisofbiomarkersinclinicallyaccessiblequantitiesofhumanaqueoushumor[J].MolecularVison,2009,15:60-69.[9]HaasnootW.Luminex-basedtripleximmunoassayforthesimultaneousdetectionofsoy,pea,andsolublewheatproteinsinmilkpowder[J].JAgricFoodChem,2007,55(10):3771-3777.[10]WaterboerT,SehrP,PawlitaM.Suppressionofnon-specificbindinginserologicalLuminexassay[J].JImmunolMethods,2006,309(1-2):200-204.[11]MartinsTB.Heterophileantibodyinterferenceinamultiplexedfluorescentmicrosphereimmunoassayforquantitationofcytokinesinhumanserum[J].ClinDiagLabImmunol,2004,11(2):325-329.[12]KrichaLJ.Humanant-ianimalantibodyinterferencesinimmunologicalassays[J].ClinChem,2004,45:942.[13]OpalkaD,LachmanCE,MacMullenSA,etal.Simultaneousquantitationoftypesantibodiestoneutralizingepitopesonvirus-likeparticlesforhumanpapilomavirustype6,11,16and18byamultiplexedLuminexassay[J].ClinDiagnLabImmunol,2003,10(1):108-115.[14]WongSJ,DemarestVL,BoyleRH,etal.Detectionofhumanant-iflaviviruswithaWestNilevirusrecombinantantigenmicrosphereimmunoassay[J].JClinMicrobiol,2004,42(1):67-72.[15]LachmannN,TerasakiPI,BuddeK,etal.Ant-ihumanleukocyteantigenanddonor-specificantibodi