多粘芽孢杆菌发酵培养基优化及发酵特性研究

FOODANDFERMENTATIONINDUSTRIES462007Vol133No17(Total235),,(,,241000),,,,30L,,,816104U/mL1219104U/mL,115,,:,():2007-01-18,:2007-04-17(Colistin)E(PolymyxinE),(po1ypeptidesystem),,,,,,,,,,,[15]1990,,,E,10105U/mL,,,,1111:(PaenibacilluspolymyxaGK21):(Escherichiacoli)112:1%NaCl0105%1%(015h)NH4Cl011%KH2PO4013%Na2HPO4015%118%,,pH:3115%017%0155%NaCl013%0101%CaCO30142%,,pH613617:1g,013g,0125g,NaCl2g,K2HPO40125g,H2O100mL,116g,pH711,011MPa20min:VI(2005,XIA)[6]113,,3622h,114(19536U/mg),(pH610),1000U/mL,1600U/mL800U/mL115BS21EAW2GJ22FDCRDX2280TDL240BWKT2J2D230L/3(6mm718mm10mm)116:[6]::117,(NH4)2SO4L24,(NH4)2SO4L24,4,2007337(235)47,L16(45)[79]2211,(NH4)2SO4L2,A2B3C3D31(A)/%(B)/%(C)/%L2(D)/%11411101201102161120130115318113014012042011401501252ABCD10-4/UmL-1112328152243391233412617442137125131471362111812731348118433181091143610102323813113342717124122710131421612142241718153224614164444614K128291329143012K23315291927192919K32819311333183017K42816281527192812k17100713371357155k28138714861987148k37123718381457168k47115711361987105R11380170114701633F418553116181419(NH4)2SO4111130137314519053119681812L201945301315219013253011081311415:F0105(3,3)=9128,F0101(3,3)=2915212,,918104U/mL,912104U/mL30L21211(1)121212(2)2212133FOODANDFERMENTATIONINDUSTRIES482007Vol133No17(Total235)123,5h,,15h,,40h,,,1219104U/mL3,,(NH4)2SO4L24:16%(NH4)2SO4113%014%L2012%,,918104U/mL,30L,816104U/mL1219104U/mL,1151Falagas,MatthewEColistin.TheRevivalofPolymyxinfortheManagementofMultidrug2ResistantGram2Nega2tiveBacterialInfections[J].ClinicalInfectiousDiseases,2005,(5):133313412.[S].,1999,(1):21233KoyamaY,AKurosasa,TsuchiyaA,etal.Anewanti2bioticcolistinproducedbyspore2formingsoilbacteria[J].JAntibiot,1990,(3):4574584SofiaKK,ArgyrisM.CombinationTherapywithIntra2venousColistinforManagementofInfectionsDuetoMul2tidrug2ResistantGram2NegativeBacteriainPatientswithoutCysticFibrosis[J].AntimicroblalAgentsandChemothrapy,2005,(6):313631465WoottonM,HoltHA,MacGowanAP.Developmentofanovelassaymethodforcolistinsulphomethate[J].ClinicalMicrobiologyandInfection,2005,(3):2432446.()[S].:,2005:XIA7,,,.[J].,2003,29(4):19238,,.rhG2CSF[J].,2003,23(11):68719,,.HL-9901[J].,1999,8(4):343347OptimizationofFermentationCultureMediumforPaenibacilluspolymyxaandStudiesonFermentationFeatureFengQuan,TangBin,ChenZhongbi(AnhuiUuniversityofTechnologyandScience,Wuhu241000,China)ABSTRACTInthispaper,orthogonaltestisdesignedtooptimizefermentationculturemediumforPaenibacilluspolymyxa.Theopticalprescriptionforfermentationwasobtainedbystatisticalanalysis.Somerelevantparametersweremeasuredandgrowth2curveswereconfirmedinthecourseoffermentationwith30Lbioreactor.Theresultshowsthatbiologicalactivitywas1219104U/mL,whichincreasedby50%.KeywordsPaenibacilluspolymyxa,orthogonaldesign,fermentationfeature,,,,,5%,,,