中国马铃薯疮痂病研究初报-赵伟全

:1000-1573(2004)06-0074-04赵伟全,　杨文香,　刘大群,　张　汀,　孟庆芳,　张丽蕊(,,071001):10,。80,、3,30。3,。　　:;;:S431.13　　　　　　:APrimarystudyonthepotatoscabinChinaZHAOWei-quan,YANGWen-xiang,LIUDa-qun,ZHANGTing,MENGQing-fang,ZHANGLi-rui(CollegeofPlantProtection,AgriculturalUniversityofHebei,BiocontrolCentreofPlantDiseasesandPlantPestsofHebeiProvince,Baoding071001,China)Abstract:Thetuberswhichdiseasedwithpotatoscabwerecollectedfrom10provincesinChina,andthescabsymptomsweredescribed.EightyStreptomycesstrainswereisolatedfromscablesions.Threekindsofthepathogenicitytestsshowedthat30Streptomycesstrainswerepathogenic.Inocu-lationwithpathogenicstrainsinducedscabsymptomsinpotato,producednecroticreactionontuberslices,andsignificantlyreducedshootheightofradishseedlings.TheresultsshowedthatthetuberslicebioassayandtheradishseedlingtestweresuitableforidentifyingpathogenicStreptomycesstrains.Keywords:potatoscab;Streptomyces;pathogenicitytest(Potatoscab),。,,。,,。,,,,。3,Streptomycesscabies、S.acidiscabiesS.turgidiscabies[1-3]。,、、。,,,。:2004-05-13:863(2003AA241130):(1975-),,,,.:(1958-),,,.E-mail:[e-mail]276200411JOURNALOFAGRICULTURALUNIVERSITYOFHEBEI　　Vol.27No.6Nov.2004,。10(),,。1　材料和方法1.1　、、、、、、、、、10()。1.2　(1992)[4]。,5mm3,(1%)30s,1mm,1.8%(1mg/LPenicillinG)28℃5～7d;OMA(1mg/LPenicillinG),3,。6～8d,OMA,8～10d,,,,4℃10000r/min15min,,1mL20%,-20℃。1.3　3。1.3.1　盆栽试验　FAUCHERetal(1992)[5]。(0.6%)10min,,,3;100mLOMA10d,15cm,,4～6;10,。1.3.2　小薯片法　LORIAetal(1995)[6]。1(4～5cm),75%,(1.5cm),2mm,,4;OMA7d0.5cm×0.5cm,;22～24℃,10d,。1.3.3　萝卜幼苗检测法　LEINERetal(1996)[7]。:15μLOMB,5d(220r/min,28℃),,,5OMB,;,0.2μm。()0.26%10min,3,,24h,10mL1%50mL,3;200μL,OMB,24℃,,16h,6d。,。,。3。2　结果与分析2.1　、、、、、、、、、、10(),。、、75　6　　:,。、、,,,;,,,30%～60%,,。,(6%～10%),。,(2),,,,(1)。1　Fig.1　ThesymptomsofpotatoscabinChina,,。,。,,;、、、,,;,,;,;,,。2.2　10。,;、(1),,。,,,,。,。。,,、、,、、、,,、、。2.3　10(),76　　　　　272　Fig.2　Theresultsofpathogenicitytest:(A):CPS-1;(B):S.lividans:(C):;1(D):;2(E):.(F)::CPS-1(1),CPS-2(2),CPS-3(3),CPS-4(4),YNZ-1(6),SC-3(7),HB-11(8),GS-1(9);:S.lividans(5,10).;3,80。,30,()3。,(2)。,10d,,,,,(2)。,,8.8%～38.3%,,,92.6%～98.5%;,,7.8%～44.4%;,89.2%～105.6%。CPS-12。3,,。3　讨论,,,S.scabies,S.acidiscabiesS.turgidiscabies3,[8,9]。,(superficialscablesions),(russetscab)(nettedscab),(raisedscablesions)(pittedscab)[10]。,[11]。10(),,、3,。,8～10,。、,。,,2,。32,。,。(下转第92页)77　6　　:3　结论,,。,,,。,,(Cf)、,,(P)(Cf):Cf=12.033P-107.47,,、,。:[1]　.[J].,1996,9:28-30.[2]　.[D].:,2003.[3]　,,,.[J].,2002,20(2):18-20.[4]　TD/T1004-2003,[S].[5]　.———[D].:,2002.[6]　,,.GIS[J].,2002,23(3):16-20.(编辑:郭桂仙)(上接第77页):[1]　LAMBERTDH,LORIAR.Streptomycesacidiscabiessp.Nov.[J].InternationalJournalofSystematicBacteriology,1989,39:393-396.[2]　LAMBERTDH,LORIAR.Streptomycesscabiessp.Nov.,nom.rev.[J].InternationalJournalofSystematicBacteriology,1989,39:387-392.[3]　MIYAJIMAK,TANAKAF,TAKEUCHIT,etal.Streptomycesturgidiscabiessp.nov.[J].InternationalJournalofSystematicBacteriology,1998,48:495-502.[4]　DAQUNLIU.BiologicalcontrolofStreptomycesscabiesandotherplantpathogens[D].UniversityofMinnesota,USA.1992.[5]　FAUCHERE,SAVARDT,BEAULIEUC.CharacterizationofActinomycetesisolatedfromcommonscablesionsonpotatotubers[J].CanadianJournalofPlantPathology,1992,14:197-202.[6]　LORIAR,BUKHALIDRA,CREATHRA,etal.DifferentialproductionofthaxtominsbypathogenicStreptomycesspeciesinvitro[J].Phytopathology,1995,85:537-541.[7]　LEINERRH,FRYBA,CARLINGDE,etal.ProbableinvolvementofthaxtominsAinpathogenicityofStreptomycesscabiesonseedlings[J].Phytopathology,1996,86:709-713.[8]　FAUCHERE,OTRYSKOB,PARADISE,etal.CharacterizationofStreptomycescausingrussetscabinQuebec[J].PlantDisease,1993,77:1217-1220.[9]　PARKDH,KIMJS,KWONSW,etal.Streptomycesluridiscabieisp.nov,Streptomycespuniciscabieisp.nov.andStreptomycesniveiscabieisp.nov,whichcausepotatocommonscabdiseaseinKorea[J].InternationalJournalofSystematicandEvolutionaryMicrobiology,2003,53:2049-2054.[10]　LOCCIR.Actinomycetesasplantpathogens[J].EuropeanJournalofPlantPathology,1994,100:179-200.[11]　LORIAR,BUKHALIDRA,FRYBA,etal.PlantpathogenicityinthegenusStreptomyces[J].PlantDisease,1997,81:836-846.(编辑:李　川)92　　　　　27