植物中绿原酸合成途径研究进展-何柳

PharmaceuticalBiotechnology2013205463～466*100193。。。。、3。。Ｒ282.6A1005-8915201305-0463-041Chlorogenicacid1。18972。3、456。。7。Niggeweg8。。910DNA11。。。3。23-O-3-O-caffeoylquinicacidC6H18O91947ＲudkinNelson112。1959Zucker、1314。PAL。323。1364*2012-04-272013-04-02010-57833143。*DNA010-62899700。2052.1A、AATP1115StckigtA15。UlbrichA∶Hydroxycinnamoyl-CoAquinatehydroxy-cinnamoyltransferaseHQTA16HQT2①。、、、HQTHQTHQT817-20。HQT。。2.2Uritani-D-D-21D-∶HCGQT-D-D-HCGQT2②22。HCGQT23。2.3CYP98A3p-3-CYP98A324-25。Hydroxycinnamoyl-CoAshikimate/quinateHydroxycinnamoyltransferaseHCTA5-O-caffeoylquinateesterA262③。CYP98A3HCT。233、。。3HQT1HQTA。HQT816-20。HQTBAHDHCT464HCT26。1HQTcDNANicotianatabacumtobHQT1311bp8LycopersiconesculentumtomHQT1293bp8CoffeacanephoraCcHQT1293bp17Cynaracardunculusvar.scolymusHQT1335bp18Cynaracardunculusvar.scolymusHQT11350bp20Cynaracardunculusvar.scolymusHQT21332bp20LonicerajaponicaLjHQT1320bp19C3'HCYP98A4924-2527。C3'H。。1955Panizzi＜5%282001Michael465%29。。2004NiggewegtobHQT10%tobHQT8。HQT1N.benthamiana3620HQT1N.tabacum1020。。、。。1BradfieldAEFloodAEHulmeACetal．ChlorogenicacidsinfruittreesJ．Nature1952170168.2OsborneTBCampbellGF．TheproteidsofsunflowerseedJ．JAmChemSoc189719487.3JohnsonGSchaalLA．Ｒelationofchlorogenicacidtoscabresis-tanceinpotatoesJ．Science1952115627.4UritaniIMiyanoM．DerivativesofcaffeicacidinsweetpotatoattackedbyblackrotJ．Nature1955175812.5．J．200728763.6JohnsonGSchaalLA．AccumulationofphenolicsubstancesandascorbicacidinpotatotubertissueuponinjuryandtheirpossibleroleindiseaseresistanceJ．AmerPotatoJ195734200.7TamagnoneLMeridaAStaceyNetal．InhibitionofphenolicacidmetabolismresultsinprecociouscelldeathandalteredcellmorphologyinleavesoftransgenictobaccoplantsJ．PlantCell1998101801.8NiggewegＲMichaelAJMartinC．EngineeringplantswithincreasedlevelsoftheantioxidantchlorogenicacidJ．NatBiotechnol200422746.9WatanabeTAraiYMitsuiYetal．Thebloodpressure-loweringeffectandsafetyofchlorogenicacidfromgreencoffeebeanextractinessentialhypertensionJ．ClinExpHypertens200628439.10NardiniMCirilloENatellaFetal．AbsorptionofphenolicacidsinhumansaftercoffeeconsumptionJ．JAgricFoodChem2002505735.11SawaTNakaoMAkaikeTetal．Alkylperoxylradical-scaven-gingactivityofvariousflavonoidsandotherphenoliccompoundsImplicationsfortheanti-tumor-promotereffectofvegetablesJ．JAgricFoodChem199947397.12ＲudkinGONelsonJM．ChlorogenicacidandrespirationofsweetpotatoesJ．JAmChemSoc1947691470.13ZuckerMLevyCC．SomeFactorswhichaffectthesynthesisofchlorogenicacidindisksofpotatotuberJ．PlantPhysiol195934108.14ZuckerMNitschCNitschJP．TheinductionofNicotiana．Ⅱ．PhotoperiodicalterationofthechlorogenicacidconcentrationJ．AmerJourBot196552271.15StckigtJZenkMH．Enzymaticsynthesisofchlorogenicacidfrom564205caffeoylcoenzymeAandquinicacidJ．FEBSLett197442131.16UlbrichBZenkMH．Partialpurificationandpropertiesofhydroxycinnamoyl-CoAquinatehydroxycinnamoyltransferasefromhigherplantsJ．Phytochem197918929.17LepelleyMCheminadeGTremillonNetal．ChlorogenicacidsynthesisincoffeeAnanalysisofCGAcontentandreal-timeＲT-PCＲexpressionofHCTHQTC3H1andCCoAOMT1genesduringgraindevelopmentinC．canephoraJ．PlantSci2007172978.18CominoCHehnAMogliaAetal．TheisolationandmappingofanovelhydroxycinnamoyltransferaseintheglobeartichokechlorogenicacidpathwayJ．BMCPlantBio2009930.19PengXXLiWDWangWQetal．CloningandcharacterizationofacDNAcodingahydroxycinnamoyl-coAquinatehydroxycin-namoyltransferaseinvolvedinchlorogenicacidbiosynthesisinLonicerajaponicaJ．PlantaMed2010761921.20SonnanteGAmoreＲDBlancoEetal．Novelhydroxycinnamoyl-coenzymeAquinatetransferasegenesfromartichokeareinvolvedinthesynthesisofchlorogenicAcidJ．PlantPhysiol20101531224.21UritaniIKojimaM．Studiesonchlorogenicacidbiosynthesisinsweetpotatoroottissueusingtrans-cinnamicacid-2-14Candquinicacid-G-3HJ．PlantCellPhysiol197213311.22VillegasＲJAKojimaM．PurificationandcharacterizationofhydroxycinnamoylD-glucosequinatehydroxycinnamoyltransferaseintherootofsweetpotatoIpomoeabatatasLamJ．JBiolChem19862618729.23StrackDGrossW．PropertiesandactivitychangesofchlorogenicacidglucaricacidcaffeoyltransferasefromtomatoLycopersiconesculentumJ．PlantPhysiol19909241.24SchochGGoepfertSMorantMetal．CYP98A3fromArabidopsisthalianaisa3'hydroxylaseofphenolicestersamissinglinkinthephenylpropanoidpathwayJ．JBiolChem200127636566.25FrankeＲHumphreysJMHemmMＲetal．TheArabidopsisＲEF8geneencodesthe3-hydroxylaseofphenylpropanoidmetabo-lismJ．PlantJ20023033.26HoffmannLMaurySMartzFetal．PurificationcloningandpropertiesofanacyltransferasecontrollingshikimateandquinateesterintermediatesinphenylpropanoidmetabolismJ．JBiolChem200327895.27MogliaACominoCPortisEetal．IsolationandmappingofaC3'HgeneCYP98A49fromglobeartichokeanditsexpressionuponUV-CstressJ．PlantCellＲep200928963.28PanizziLScarpatiMLOrienteG．ThesynthesisofchlorogenicacidJ．Experientia195511383.29SefkowM．FirstefficientsynthesisofchlorogenicacidJ．EurJOrgChem200161137.ＲesearchProgressesonSynthesisofChlorogenicAcidinPlantsHELiuCHENShi-lin*InstituteofMedicinalPlantDevelopmentChineseAcademyofMedicalSciencesandPekingUnionMedicalCol-legeBeijing100193ChinaAbstractChlorogenicacidCGAisthemostwidesp