CK19、CK20在甲状腺乳头状癌诊断中的应用价值

CK19CK2012111CK19CK207015343Warthin211510105CK19CK20CK195553215132P0.0510105CK20CK19CK20R736.1A1001-7399200202-0181-04Diagnosticutilityofcytokeratin1920inthepapillarycarcinoLaofthethyroidglandZhangJing1QiuJushi2ChenJinpei1LiWen1LiuGuorong11DepartmentofPathologyNo1MunicipalHospitalofGuangzhouGuangzhou5101802DepartmentofPathologySunYat-senUniversityofMedicalSciencesGuangzhou510089ABSTRACTPurposeToanalysetherelationbetweentheexpressionofCK19CK20andthyroidcarcinomas.MethodsIm-munohistochemicalstainingwasperformonformalin-fixedparaffin-ermbededsectionsfrom70carcinomas10follicularadenomas10multinodulargoiters5Hashimotothyroiditis.ResultsAstoimmunohistochemicalreactiontoCK19of55papillarycarcinomas53showedmoderatetostrongpositive2showedweakpositive.Of15follicularcarcinomas13werenegativetoweakpositive2weremoderatetostrongpositive.TheyshowedremarkablydifferenceP0.05.Follicularareasofsurroundjngthyroidcarcinoma10follicularadenomas10multinodulargoiters5Hashimotothyroiditiswerealmostnegativetoweakpositiveandonlyminorityshowedmoderatepositive.AsforimmunohistochemicalreactiontoCK20allcasespapillarycarcinomafollicularcarcinomasur-roundingfollicularofcarcinomafollicularadenomasmultinodulargoitersHashimotothyroiditiswerenegativetoweakpositiveandhenceitdidnotshoweddistinction.ConclusionsCK19maybehelpfulindifferentiatingpapillarycarcinomafrompapillaryhyper-plasticmultifocalfollicularcarcinomaandallbenignfollicularlesions.CK20maynotbehelpfulindifferentialdiagnosisofthyroidlesions.KEYWORDSthyroidneoplasmscarcinomapapillarykeratindiagnosisdifferential2001-09-141510180251008937Tel[phone]-70966CK1CK1920CK19CK2023CK19CK20181JClinExpPathol2002Apr18211.119956~200169547015346Warthin32115581212~7635~551010510%5μm1.2CK19CK20DakoHES-P1.301%~10%1+11%~50%2+50%3+1.4x222.1CK1915CK1911321234248326CK1961576111CK19S-P*1002CK19S-P*1003CK19S-P*2004CK19S-P*100281JClinExpPathol2002Apr1821CK19nCK1901+2+3+1500213340282460024157611104420103430511300~1+2+3+P0.05P0.05P0.0510442103435113CK1942.2CK2015104134239116151131CK20135CKCK19Rapheal3CK19Zubair6CK19CK20CK19CK20Fonse-ca7CK19CK19Miettinen5CK1989CK19CK19CK193Warthin21CK19CK19CK19CK19CK19Zubair6CK19CK19CK1910PasqualeCK19381JClinExpPathol2002Apr182CK20Weber11RT-PCRCK20mRNACK19CK201PermanetterWNathrathWBL.Immunohistochemicalanalysisofthyroglobulinandkeratinbenignandmalignantthyroidtumorsandfollicularthyroidadenoma.VirchowsArch1982398221~82SchelfhoutLJDMVanMuijenGNPFleurenGJ.Expressionofkeratin19distinguishespapillarythyroidcarcinomafromfollicularthyroidcarcinoma.AmJClinPathol198992654~93RaphealSJMckeown-EyssenGAsaSL.High-molecularweightcytokeratinandcytokeratin-19inthediagnosisofthyroidtumor.ModPathol19947295~3004..219981299~3045MiettinenMKovatichAJ.Keratinsubsetsinpapillaryandfollicu-larthyroidlesionsAparaffinsectionanalysiswithdiagnosisimpli-cations.VichowsArch1997431407~136ZubairWBalochMDAbrahamSetal.DifferentialexpressionofcytokeratininfollicularvariantofpapillarycarcinomaAnim-munohistochemicalstudyanditsdiagnosticutility.HumPathol1999301166~717FonsecaENeslandJMHZieJetal.Patternsofexpressionofin-termediatecytokeratinfilamentsinthethyroidglandAnimmuno-histochemicalstudyofsimpleandstratifiedepithelial-typecytoker-atins.VichowsArch1997430239~468..2000166510~29.367.2001173238~4010DiPas[ualeMRothsteinJLPala\\oJP.PathologicfeaturesofHashimotos-associatedpapillarythyroidcarcinoma.HumPathol200132124~3011WeberTLacroixJWeit\J.Expressionofcytokeratin20inthy-roidcarcinomasandperipheralblooddetectedbyreversetranscrip-tionpolymerasechainreaction.BrJCancer2000821157~60PAPR361]2B1001-7399200202-0184-01PAPPAPPAP2001^12^0521000230Tel[phone]E-mailj\[e-mail]2_1~2_1]5PAP121~2mm3Triton‘-1004481JClinExpPathol2002Apr182