高效液相色谱-紫外检测法测定食品中的过氧化氢

26,2Vol.26,No.220093ChineseJournalofSpectroscopyLaboratoryMarch,2009-,:[phone];E2mail:[e-mail]:(1979),,,,:2008210214;:2008210227(228315700)60,,0.45Lm200nm,0.5mLömin,,2,C181.510-7molöL,R75%,RSD3%,,:O657.7+2:B:100428138(2009)02204142041(H2O2),,,,,,,[1],1.4mgöm3[2],[3],,,,[4][5]2,2222225,52,[6],,,,,22.1HP1100,(UV)HPChenstationG1311AG1311AG1316A();SK3200HP();Milli2Q(Millipore);();0.45Lm13mm();3K15(Sigma)(Tedia),10%(,),20%(,),,(,)2.2:ZorbaxC18(250mm4.6mmi.d.,5Lm);1090(VV);:0.5mLömin;:200nm2.3:H2O2(30%)10,,:(,)5.0g50mL,,6020min3mL3mL,6000römin,33.1:ZorbaxC18(250mm4.6mmi.d.,5Lm),ZorbaxC8(250mm4.6mmi.d.,5Lm)NucleosilC18(250mm4.6mmi.d.,5Lm),3,ZorbaxC18,:22,,,200nm,205nm195nm,1.00.80.5mLömin,1.0mLömin0.8mLömin,3min,0.5mLömin,11;2H2O23.2,,,pH5.5,[7]3.3,,14.88min,3.3.11.02.04.06.08.010.0mL,,5142:2(X,molöL)(A),:A=154.28+218240.66X,r2=0.99911010000(mAuS,mAu,S)1030Lg3.3.2,,3,1.510-7molöL(20LL),1.0ng3.3.3,10mgökg50mgökg100mgökg,3,1231(n=3)(mgökg)r(%)RSD(%)1092.82.35095.71.510096.31.21078.13.55083.62.310088.52.121,2;3H2O231,2,4,5;3H2O23.42,,,,,,()[1],,.[J].,2006,23(3):258261.[2].[M].:,1991.12.[3].[S].GB276021996.:,1996.[4],.[J].,2007,17(3):166170.[5],.2[J].,2005,23(4):366369.[6],,.[J].,2003,21(5):524526.[7],,.[J].,2006,(1):4748.61426DeterminationofPeroxidesinFoodSamplesbyHighPerformanceLiquidChromatographywithVariableWavelengthDetectorCHENYi2huiLIUYanZHOUJian2LiZHUHai2WeiXIANGLi2Xia(XiangshanEntry2ExitInspectionandQuarantine,Xiangshan,Zhejiang315700,P.R.China)AbstractHydrogenperoxide(H2O2)wasdeterminedwithoutderivatizationbyreversedphase2highperformanceliquidchromatographic(RP2HPLC).AZorbaxODScolumn(250mm4.6mmi.d.,5Lm)wasusedwithacetontrile2water(1090,VöV)asmobilephaseatflowrateof0.5mLömin,anddetectedat200nm.Thebaselinenoisewasreducedbecauseacetontrile2waterhadmuchlowerabsorptionat200nm,andthesensitivitywasthusimproved.ThelimitofdetectionofH2O2was1.510-7molöL.Theaveragerecoveryismorethan75%andrelativestandarddeviationislessthan3%.KeywordsHighPerformanceLiquidChromatography,FoodSamples,HydrogenPeroxide.!(50%-75%)48,(20%-45%)14,(0-7%)1530,,,,,,,,,,,,,,,,,,;(),,,;,Word,[E2mail:(1)[e-mail];(2)[e-mail];(3)[e-mail];(4)[e-mail]],,16(),5,,,,,,(),,7142:2