一个与水稻条纹叶枯病抗性基因紧密连锁的分子标记的鉴定

JournalofAgriculturalBiotechnology2009,17(1):144~147*No.nyhyzx07-051、No.2006BAD02A16No.2006BAD08A04、(No.cx[08]129)、(No.BE2005301)333No.BK2005105。**。Authorforcorrespondence.。E-mail:[e-mail].2008-04-182008-05-20··***210014(Oryzasativassp.japonica)88883F2。F2F2:3RAPDOPO1188。3388RAPDOPO11883。S188A1006-1304(2009)01-0144-04IdentificationofAMarkerCloselyLinkedtoRiceStripeDiseaseResistanceGeneinaJaponicaRiceVarietyZHOUTong,WANGLei,CHENGZhao-bang,CHENJie,FANYong-jian,ZHOUYi-jun**InstituteofPlantProtectionJiangsuAcademyofAgriculturalSciences,Nanjing210014,ChinaAbstractMarkersrelatedtothegeneofricestriperesistanceinOryzasativassp.japonicaZhendao88werescreened.TheinheritanceofricestriperesistanceinF2linesandF2:3linesfromthecrossZhendao88/WuyujingNo.3werestudiedusingtillerinoculationandinoculationattheseedingstage,respectively.AmarkerOPO11,whichwascoseperatedorcompletelylinkedtoricestripediseaseresistancegeneinZhendao88,wasobtainedbyRAPDanalysis.TheamplificationresultsofOPO11primerinseveralresistancejaponicavarieties,suchasXudaoNo.3,originatedfromZhendao88,werethesameasthatofZhendao88.TheOPO11markerlinkedtothericestriperesistancegeneinZhendao88couldbeusedinmarker-assistedselectedprogramforricestriperesistancebreeding.Keywords:Ricestripevirus;ricecultivar;resistance;molecularmarker(ricestripedisease)2090(2003)。(20032003)(2006)。88(2007)。(MAS)。8888。Oryzasativassp.japonica)3881:F2。2006、F1F2。3。RAPD。F232007。20054~5(LaodelphaxstriatellusFallén,SBPH)3(,2003)2~350%RSVH550%。883/88F2F23。(8cm,45cm)。42~4H72h、。7d3d15。、F1F2:31000mL25。1.5~2.02d20。42~4H。2。72h。7d3d15。(2007)2~4010~10%10.1%~30.0%30.1%。Dellapportaetal.(1983)DNA。OperonRAPDOPO11Williamsetal.(1990)RAPD。MAPMAKER3.0(LanderandGreen,1987)。F21。388F188。F23∶1881。373.3%886.7%F15.0%5.3%88。F2:31。3/88F23、58、10141χ21∶2∶1881。RAPDOPO11388。OPO11910882500bp1。F2200RAPDParentorcrossP13(P1)WuyujingNo.3F1(P1×P2)F2(P1×P2)F1(P2×P1)P288(P2)Zhendao88Numberofresistantplants8181581928Numberofsusceptibleplants2214212Expectedratio3∶1χ21.50Table1Resistancesegregationofcrosscombinationandχ2testχ20.05,1=3.841452009Variety3XudaoNo.34XudaoNo.44LianjingNo.488Zhendao88Numberofresistantplants52575458Numberofsusceptipleplants4544/%Incidence7.18.16.96.5ResistanceleverResistanceResistanceResistanceResistance2MAPMAKER/EXP3.0RAPDOPO110。1.3/88F23Fig.1.FrequencydistributionofF23lines(n=200)withdifferentricestripediseaseincidence,derivedfromthecrossbetweenWuyujingNo.3andZhendao883、44388388,C488412934883114。RAPDOPO113、44333882。。3.RAPDOPO11Fig.3.AmplificationresultsofOPO11primerin5japonicavarietiesM,DNAmarker1,32,883,44,35,4。M,DNAmarker;1,WuyujingNo.3;2,Zhendao88;3,LianjingNo.4;4,XudaoNo.3;5,XudaoNo.4.11stvb-i。8888Modanstvb-i(Washioetal.,1968a,b;,2005)。、Table2ResistanceofXudaoNo.3,XudaoNo.4andLianjingNo4toricestripedisease2.OPO113/88F2Fig.2.SegregationofOPO11primerinF2populationM,DNAmarker1,32,883,F14~16,。。M,DNAmarker;1,WuyujingNo.3;2,Zhendao88;3,F1;4~16,F2plants.Aarrowmeansthedifferencebandbetweenparents(fatherandmother)．1461:ChenZhB(),YangRM()andZhouYJ().Thinkaboutthecontrollingstrategyforricestripe(J).JiangsuJournalofAgriculturalSciences(),2003,(suppl):3~5(inChinesewithEnglishabstract)ChoJH,KwakDY,YiKH,WeonYJ,KoJM,ParkHM,YangSJandNamMH.IdentificationandmappingofRAPDmarkerslinkedtoRicestripevirusresistancegene,Stvb-i,inrice(O.sativaL.).FischerT,TurnerN,AngusJ,McIntyreL,RobertsonM,BorrellAandLloydD.Proceedingsofthe4thInternationalCropScienceCongress(C),Brisbane,Australia,theRegionalInstituteltd,2004.DellapportaSL,WoodJandHicksJB.AplantDNAmini-preparation:Version(J).PlantMolecularBiologyReporter,1983,1:19~21LanderESandGreenP.Constructionofmultilocusgeneticmapsinhumans(J).ProceedingofNationalAcademyofSciencesoftheUSA,1987,84(4):2363~2367MaedaH,MatsushitaK,IidaSandSunoharaY.CharacterizationoftwoQTLscontrollingresistancetoRicestripevirusdetectedinaJapaneseuplandriceline,Kanto72(J).BreedingScience,2006,56:359~364PanXB(),LiangGH(),ChenZhX()andZhangYF().BreedingstrategyofricewithresistancetoricestripediseaseinJiangsu(J).JiangsuJournalofAgriculturalSciences()2005(5)22~23(inChinese)SunDZ().Inheritanceanalysisandgenemappingofresistancetoricestripe(D).Nanjin:NanjinAgricultureUniversity,2006:23~31(inChinese)WangL().Geneanalysisofricestriperesistanceinricecultivars(D).Yangzhou:YangzhouUniversity,2007:39~40(inChinese)WashioO,EzukaAandSakuraiY.Studiesonthebreedingofricevarietiesresistanttostripedisease.GeneticstudyonresistancetostripediseaseinJapaneseuplandrice(J).JapanJournalofBreeding,1968a,18:96~101WashioO,EzukaAandSakuraiY.Studiesonthebreedingofricevarietiesresistanttostripedisease.Geneticstudiesonresistancetostripediseaseinforeignvarieties(J).JapanJournalofBreeding,1968b,18:167~172WilliamsJG,KubelikAR,LivakKJandRafalskiJAandTingeySV.DNApolmorphishismsusingPCRwitharbitraryprimersareusefulasgeneticmarkers(J).NucleicAcidsResearch,1990,18(22):6531~6535ZhouT(),JiJA(),ZhouYJ(),ChenZB(),ZhangYG()andXuXL().Preliminaryreportonfieldevaluationofresistancetoricestripeinricevarieties(J).JiangsuJournalofAgriculturalSciences(),2006,22(