C6大鼠脑胶质瘤动物模型建立及病理观察_张荣

201381WorldJournalofIntegratedTraditionalandWesternMedicine2013Vol.8No.1··[e-mail]6【】、C6。C62．5×106·25μL－1SD7、14、21dHE。C67d14d21d。183100%。HE。C6、。【】【】R730．264【】A【】1673－6613201301－0073－03AnimalModelEstablishmentandPathologicalObservationofC6CerebralGliomainRatsZHANGRongDUANMei－meiInstituteofClinicalPharmacologyGuangzhouUniversityofTraditionalChineseMedicineGuangzhouGuangdong510405【Abstract】ObjectiveToestablishthesimplefeasiblereliableandstableC6cerebralgliomamod-elinratssoastoprovidetheplatformforthestudyofthepathogenesisandthemethodofpreventionandtreatmentofcerebralglioma．MethodsThestereotaxictechniquewasadopted．C6gliomacellsinratscul-turedinvitrowerecondensedandplacedinsuspension．C6gliomacellscontaining2．5×106C6cells/25μLwereimplantedintotherightcaudatenucleusinSDrats．Thesurvivalconditionsoftheratswereconsec-utivelyobserved．Separatelyinthe7th14thand21stdaysoftheimplantationtheratsweresacrificedandthebrainswerecollectedtopreparethepathologicalsections．WithHEstainingtheywereobservedunderlight－microscope．ResultsInabout7daysafterC6gliomacellsimplantedthesurvivalstateofratswasgood．Inabout14daystheintracranialhypertensionwasapparent．Inabout21daysmostoftheratswereincriticalcondition．Inthegrossspecimenexaminationexcepttheaccidentaldeathof3ratsthetumorformationratewas100%intherestof18rats．Alongwiththeagingoftheratsthetumorsizewasenlargedthemidlinestructurewasmigratedobviouslyandtheoccupiedeffectwasgettingmarked．HEstainingdiscoveredthesig-nificantformationofcerebralgliomaintherats．ConclusionTheanimalmodelofC6ratcerebralgliomaisreliableandstable．Thetumorgrowthpropertyandpathologicalcharacteristicsaresimilartohumancerebralglioma．Hencethemodelisapplicablesatisfactorilyfortheclinicalbasicresearchofglioma．【Keywords】CerebralgliomaStereotaxicTechniqueAnimalModelPathology35%～60%。、。1－4C6SD。。、1．C6100mL·L－1·37·201381WorldJournalofIntegratedTraditionalandWesternMedicine2013Vol.8No.1RPMI－1640SD180～220gSCXK2008－0002。2．MCO175SanyoBeckmanCS－15ROlym-pus25μLQ/AMGK01－1998、F12K、、Gibco10%AR。、1．C615%2．5%90%0．25%F12K。C6＞90%800rpm·min－14min1×1011·L－137℃。2．12h10%3．5mg·kg－11．5cm×1．5cm1cm1．0mm、3．0mm1．0mm。25μLC65．5mm1mm10min10min。。3．、、7、14、21d－5。1．C6181116d157、14、21d5。2．1d2d1。12。21。1C6x±s7d14d21dg5209．80±12．84211．70±8．26201．00±2．16167．25±7．18ab7daP＜0．0114dbP＜0．013．100%。。。HE。1。4C6HE×200·47·201381WorldJournalofIntegratedTraditionalandWesternMedicine2013Vol.8No.1、。、、、、。VottorionM612345。、7。、。、/、。、、、、、。C6＞90%37℃90min1×106·10μL－1、2．5×106·25μL－1、4×106·25μL－1、6×106·μL－1C625μL2．5×1061mm3mm5．5mm6cm。C65．5～6mm1mm。25μL10min10min8。。。9。7d7～14d、21d、、、8～10g·d－1。10～14d。、、。1GrobbenBDeDeynPPSlegersHetal．RatC6gliomaasexperimen-talmodelsystemforthestudyofglioblastomagrowthandinvasionJ．CellTissueRes20023103257－270．99·57·201381WorldJournalofIntegratedTraditionalandWesternMedicine2013Vol.8No.1。、。、、。、。。、、、、。。1GhadiallyRBrownBESequeia－MartinSMetal．Theagedepider-malpermeabilitybarrierstructuralfunctionalandlipidbiochemicalabnormalitiesinhumanandasenescentmurinemodelJ．JClinIn-vest19959552281－2290．2XuLOlivryTChanLS．MolecularcloningofacDNAencodingtheporcinetypeXVIIcollagennoncollagenous16Adomainandlocaliza-tionofthedomaintotheupperpartofporcineskinbasementmem-branezoneJ．VetDermatol2004153146－151．3．ESJ．1992196491－496．4KosterMIKimSMillsAAetal．p63isthemolecularswitchforini-tiationofanepithelialstraticationprogramJ．GenesDev2004182126－131．5DelaurenziVRossiATerrinoniAetal．p63andp73transactivatedifferentiationgenepromotersinhumankeratinocytesJ．BiochemBiophysResCommun20002731342－346．6KinoshitaNTsudaMHamuyRetal．Theusefulnessofbasicfibro-blastgrowthfactorforradiation－exposedtissueJ．WoundRepairRegen201220191－102．7．J．200711469400－9403．8．J．200913193689－3692．9．J．200420516－18．10．J．20112261385－1387．2012－08－14752．J．2005125311－314．3．C6J．2000213307－310．4WatanabeKSakamotoMSomiyaMetal．FeasibilityandlimitationsoftheratmodelbyC6gliomasimplantedatthesubcutaneousregionJ．NeurolRes200224485－490．5DelDucaDWerbowetskiTDelMaestroRF．Spheroidpreparationfromhangingdropscharacterizationofamodelofbraintumorinva-sionJ．JNeurooncol200467295－303．6VottorioMMorrealeVMHermanBHetal．Abraintumormodeluti-lizingstereotactimplantationofapermanentcannulaJ．Neurosurg199378959－965．7．J．2012333337－342．8．C6J．200833101260－1263．9．J．2004244331－334．2012－11－05·99·