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pFastBacHTA编号 载体名称 北京华越洋生物KCVECT130 pFastBacHT A pFastBacHTA载体基本信息载体名称:pFastBacHTA,pFastBacHTA,pFastBacHTA,pFastBacHTA质粒类型:昆虫细胞表达载体；杆状病毒表达载体启动子:Polyhedrin表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:4856bp5'测序引物:pFastbac-F:TATTCCGGATTATTCATACC3'测序引物:pFastBac-R:ACAAATGTGGTATGGCTGA载体标签:N-Histag载体抗性:Ampicillin氨苄筛选标记:Gentamicin庆大霉素备注:含TEV切割位点稳定性:稳表达Stable组成型:组成型Constitutive病毒/非病毒:杆状病毒pFastBacHTA载体质粒图谱和多克隆位点信息 pFastBacHTA载体简介 Bac-‐to-‐BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后，在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具 有以下特点： ⇒用于表达蛋白质的多角体强启动子 ⇒用于简化克隆的三个读码框 ⇒用于轻松纯化重组融合蛋白的 N-‐端 6xHis 标签 ⇒用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点 引用及参考文献 DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-‐rich Aggregation-‐prone Region and Forms Cytoplasmic Granules that Co-‐localize with P-‐bodies. Hey F, Czyzewicz N, Jones P, Sablitzky F, J Biol Chem (2012) 287:31073-‐31084 Product usage: na Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin. Dan C; Kelly A; Bernard O; Minden A; J Biol Chem (2001) 276:32115-‐32136 Product usage: pFASTBAC/HT and pFASTBAC-‐1 vectors were used to transfect with Sf9 cells with Cellfectin. Sp3 Represses Gene Expression via the Titration of Promoter-‐specific Transcription Factors. Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M; J Biol Chem (2002) 277:9780-‐9789 Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-‐Sp1/flu, pBSK-‐Sp3/flu, pCR-‐M1/flu, and pCR-‐M2/flu as substrates. PCR reactions employed pGEX-‐M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA. pFastBacHTA载体序列 ORIGIN1GACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACC61GCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCC121ACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTT181AGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG241CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGT301GGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTA361TAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT421AACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAAT481GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG541AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA601CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC661CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC721ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT781CCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC841GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA901CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC961ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG1021GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA1081CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG1141GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA1201TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG1261GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT1321GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT1381CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG1441CATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAT1501TTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCT1561TAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCT1621TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA1681GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTC1741AGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTC1801AAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCT1861GCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAG1921GCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACC1981TACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGG2041AGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAG2101CTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTT2161GAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAC2221GCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCG2281TTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGC2341CGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATG2401CGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCT2461GGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGA2521CAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAG2581ACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTT2641TGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGA2701GGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAAC2761AACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGG2821TCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCG2881GGATCGTCACCGTAATCTGCTTGCACGTAGATCA