玉米杂交种掖单13号的SSR连锁图谱构建与叶夹角和叶向值

HEREDITAS(Beijing)2007929(9):1131―1138ISSN0253-9772研究报告收稿日期:2007−04−13;修回日期:2007−05−13作者简介:(1978−),,,E-mail:[e-mail]通讯作者:(1948−),,,,,,Tel:[phone],Fax:[phone];E-mail:[e-mail]:10.1360/yc-007-113113SSRQTL路明1,周芳2,谢传晓1,李明顺1,徐云碧3,MarilynWarburton3,张世煌11.,100081;2.,110161;3.,:为了增加单位面积产量,玉米育种者已经开始了更密植更紧凑株型的选育。叶夹角和叶向值是评价玉米株型的重要指标。本研究以掖478×丹340的500个F2单株为作图群体,构建了具有138个位点的SSR标记连锁图谱,图谱总长度为1394.9cM,平均间距10.1cM。利用397个F2：3家系对叶夹角和叶向值进行QTL定位分析,结果表明:叶夹角和叶向值分别检测到6和8个QTL,累计解释表型变异41.0%和60.8%,单个QTL的贡献率在2.9%~13.6%之间。与叶夹角和叶向值有关的基因主要作用方式为加性和部分显性。此外两个性状共检测到9对上位性互作位点,表明上位性互作在叶夹角和叶向值的遗传中也起较重要的作用。:玉米;叶夹角;叶向值;QTLConstructionofaSSRlinkagemapandmappingofquantitativetraitloci(QTL)forleafangleandleaforientationwithanelitemaizehybridLUMing1,ZHOUFang2,XIEChuan-Xiao1,LIMing-Shun1,XUYun-Bi3,MarilynWarburton3,ZHANGShi-Huang11.MaizeCenter,InstituteofCropScience,ChineseAcademyofAgriculturalSciences,Beijing100081,China;2.MaizeResearchInstitute,ShenyangAgricultureUniversity,Shenyang110161,China;3.InternationalMaizeandWheatImprovementCenter,MexicoAbstract:Maize(ZeamaysL.)breedershavebegunselectingformorecompactplantsforhigherdensityplantinginordertoincreaseyieldperunitarea.Leafangleandleaforientationareveryimportanttraitsaffectingmaizeplanttype(compact-ness).Inthisstudy,ageneticlinkagemapcontaining138simplesequencerepeat(SSR)markerswasconstructedbasedonamappingpopulationconsistingof500F2individualsfromthecrossbetweeninbredlinesYe478andDan340.ThisSSRlinkagemapspansatotalof1394.9cMwithanaverageintervalof10.1cM.Quantitativetraitloci(QTL)forleafangleandleaforientationwereidentifiedin397F2:3families.SixQTLforleafangleweredetectedthatcouldexplain41.0%ofthephenotypicvariation;while,eightQTLweredetectedforleaforientationthatcouldexplain60.8%ofthephenotypicvaria-tion.SingleQTLcontributiontophenotypicvariationrangedfrom2.9%to13.6%.Additiveandpartialdominancewerethemaingeneticeffectsforleafangleandleaforientation;inaddition,ninepairsoflocusinteractionsweredetectedforthetwotraits,indicatingthatepistaticinteractionsatthetwo-locilevelalsoplayameasurableroleinthegeneticbasisofthetwo1132HEREDITAS(Beijing)200729traits.Keywords:maize;leafangle;leaforientation;QTL,,[1],MockPearce[2]1975,[3~8],,,,13,[9]1.22006397F1,2020α-Latice,5,0.60,,3,165(Leafangle,LA),(leaforientation,LO),Pepper[10]:1(90)(/)/nifiiLOLLn−=−θ∑,θii(),Lf(cm),L(cm),n,,,Pepper[10],[10~12],,1.3SSRF2,DNACTAB[25]IBM200410SSR,MaizeGDB()SSR,,SSRPCR,CIMMYTAppliedMolecularGeneticsLaboratory142SSR,F2(1:2:1),MAPMAKER/EXP3.0b[26](LOD3.0),Kosambi[27](centiMorgan,cM)20,QTL,QTL[13~23]QTL[24]QTL,,11.11.413478340,2004,F12005,F2500DNA,F2,3972005,,QTLSAS[28]PROCMIXED,,SASPROCUNIVARIATEPROCCORR1.5QTLwinQTLCart2.5[29]QTL,9:13SSRQTL1133(CIM)Model6,5,10.0cM,LOD1000QTLStuber[30]EPISTACY2.0[31]142SSRF2,,F214345P0.05(31.5%)3,140SSR,11,2137SSR138,10,1394.9cM,10.1cM(1)7umc1865phi08222.1478340500F2,图1玉米SSR标记连锁图谱及LA和LO的QTL定位Fig.1GeneticmapwithSSRmarkersandQTLmappingforLAandLOinmaize1134HEREDITAS(Beijing)200729,55.2cMIBM2,IBM2,phi042(Bin9.04)3.09,phi088(Bin3.08)3.01,phi438301(Bin4.05)4.07,phi323152(Bin10.05)10.07,phi0922,phi092a2.042.05,phi092b4.082.2F2:3:2,QTL1,,,F2:3,−11,,−0.5392.3QTLwinQTLCart2.5[29]CIMQTL(2),1000,P=0.05,LOD3.53.46QTL,1235,2.9%11.2%,41.0%13QTL,10.8%11.2%8QTL,2389,QTL4.5%13.6%,62.8%23QTL,10.9%13.6%QTL,LA3aLO2aLO2bStuber[30]QTL,5QTL,13QTL,3,2,QTL,2,2phi402893-bnlg10173bnlg2241-bnlg197,LA2LO2aLA3bLO3,LA3bLO3,QTL2.4P0.001,SASProcGLM,18(3)38,5,3phi088umc13943.7cM,8phi015umc16632.7cM,umc1663phi2333760.8cM,,AA,,,1018,10924QTL2,QTL3:SS;,SN;,NN5QTL,6787,4NN,1SN,SS4QTL,13810,43SN,1NN,SS,4.5%6.1%,LALO表1叶夹角和叶向值的表型值和变异Table1EstimatesofphenotypicvalueandvariationforleafangleandleaforientationF2:3F2:3populationTrait478Ye478340Dan340F1aMeansRangeCV%SkewnssKurtosisLA20.2322.4623.0225.100.1915.69−38.3215.110.180.06LO60.1037.6041.5244.710.3921.79−66.717.23−0.020.05a:aStandarderrorsareattachedbehindthemeans.9:13SSRQTL1135表2叶夹角和叶向值的QTL定位及遗传参数Table2Chromosomiclocation,effectsandtypesofindividualgeneactionofQTLsforleafangle(LA)andleaforientatiom(LO)TraitQTLaMarkerintervalLocation(cM)LODAD|D/A|bGeneactionR2(%)LALA1aumc1269-bnlg142936.913.82−1.210.650.53PD3.64LA1bbnlg1331-phi308707169.7111.61−1.61−0.460.28PD10.82LA2phi402893-bnlg101711.813.72−1.100.610.56PD2.86LA3aphi374118-umc177381.814.350.570.821.43OD3.77LA3bbnlg2241-bnlg197124.8111.961.820.550.30PD11.21LA5umc1822-phi04893.8110.001.510.310.21PD8.65Sum7.823.4040.95LO2aphi402893-bnlg101710.015.83−0.97−1.851.90OD4.49LO2bbnlg1017-bnlg12530.314.62−0.68−2.543.72OD4.96LO2cphi109642-bnlg10873.3113.634.34−1.380.32PD10.92LO3bnlg2241-bnlg197124.8114.744.000.260.07A13.57LO8aphi115-umc185819.918.682.850.110.04A6.88LO8bbnlg666-bnlg24032.518.852.660.290.11A7.69LO8cumc1384-umc166386.419.063.34−2.190.66PD7.06LOLO9bnlg1289-phi23665477.116.28−2.901.060.37PD7.26Sum21.79.6762.84aQTL;a,bQTLbA,PD,D,ODa:ThenumberfollowingthetwolettersrepresentsthechromosomelocationoftheQTLandisdenotedusingcontinuouslowercaseletters(abc...)follo