ELISA 使用宝典

ELISAELISAELISA(Enzyme-LinkedImmunosorbnentAssay)70ELISAELISA1.2./3.ELISAELISAELISA1.Abs2.nFInFLnFPnFRET3.nn1.2.3.ELISA1.2.3./,•(HighBindingELISAPlate):,400-500ngIgG/cm2,10kD.,,.,,.•(MediumBindingELISAPlate):,20kD,200-300ngIgG/cm2.,,..•(AminatedELISAPlate):,..pH,.,Triton-100Tween20.,;,.,succinimidealdehydemaleimideELISAUV()nUvn260nm/280nmnUV150RMBnpurifiedantigennnrecombinantantigenpeptideantigennncoating1.pH9.62.ELISA41.2.3.4.5.ELISAHRP1.HRP2.1844kD3.HRPHRPDH2H2O2HRPH2O2H2O2HRPELISAorthopenylenediamineOPD3355-tetramethylbenzidineTMBABTS[22-azino-bis(3-ethyl-benzthiazolinesulfonate-6)]OPDHRPOPDHRPAPnnnnHRPAP1.APHRP2.APHRP3.APHRP4.APHRP5.ELISAHRPnnELISA1.1010.1g/mlELISA2.3.1:10001:50001:250004.A5.A0.8A0.11g/ml1:5000nnnnnnnnn45nnnnnELISAnn20ELISAnELISAn1.2.3.2min4.3456BioTekELX40596/384nELISAnELISAnnInCellWesternnLuminexn…….nnn20S/nELx405nBioTekn75%nMDCnnTecannnnELISAnMDØnBioTekØMD•PEØ•TecanØ1.BioTekGen52.MDMaxPro6.03.BioTekKC4KCjuniornnnELISAnELISAELISAEXEM1.2.3.EXEM:1.0.05MPH9.110gml0.1ml433()2.0.1ml371()3.()0.1ml370.514.TMB0.1ml3710305.2M0.05ml6.“+”“-”O·DELX808450nm(ABTS410nm)O·DOD2.11.n(PH9.60.05M):nNaCO1.59nNaHCO2.93n1000ml(PH7.4PBS)0.15MnKH2PO40.2nNa2HPO·12H2O2.9nNaCl8.0nKCl0.2nTween-200.050.5mln1000ml(BSA)0.1100ml510(2MHSO178.3ml(98)21.7ml(PH5.0):0.2MNaHPO(28.4L)25.7ml0.1M(19.2L)24.3ml50mlTMB():TMB(10mg5ml)0.5ml(PH5.5)10ml0.75HO32lABTS:ABTS0.5mg(PH5.5)1ml3HO2l1.()[e-mail]