ARMS引物扩增受阻突变体系PCR技术

:100021336(2004)0620514202SNP:PCR111,2(1,210095;2,430070):PCR,,:PCR:Q81:2004209213,(2002)12326:(1977),,;(1962),,,,,E2mail:[e-mail](singlenucleotidepolymorphism,SNP),SNP,,SNP[1],PCRPCR(directanalysis,DA)(RFLP)(SSCP)(heteroduplexanalysis,HA)(ASA)DNASNP,[2],,,,Tetra2primerARMSPCR,PCR(tetra2primeramplificationrefractorymutationsystemPCR),PCR(amplificationrefractorymuta2tionsystem,ARMS)PCR(tetra2primerPCR),[3]1.Tetra2primerARMS2PCRPCRDNA,:TaqDNA35,3,,,3,,,DNA3;PCR,DNA3[3],,,PCR,,PCR,,GA(1),()()GA,PCRGA(),PCR,,2.Tetra2primerARMSPCR3,,;,3,,Tetra2primerARMSPCRSNPDNA4152004246CHEMISTRYOFLIFE2004,24(6)TechniquesandMethods©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[1,3]3,,3(A/GC/T)(C/AG/T)3,;,3(A/A,C/C,G/GT/T)[4]ABCA1I823MARMSPCR,,[5,6]3.Tetra2primerARMSPCR,PCR,Mg2+,TaqDNA,PCR,PCRPCRTaqDNA[1]Ye[3],101PCR[6],,0.6mol/L0.4mol/L,,TaqDNAPCR4.Tetra2primerARMSPCR,SNP,3,1,PCR;GA,GAGA;,,,GAGA;,PCR,Tetra2primerARMSPCR,PCR,;5152004246CHEMISTRYOFLIFE2004,24(6)©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[1].,2003,25(2):198200[2].,2000,22(4):392394[3]YeSetal.NucleicAcidsRes,2001,29(17):E888[4]LittleS.In:TaylorG.ed.LaboratoryMethodsfortheDetectionofMutationsandPolymorphismsinDNA,CRCPress,BocaRaton,1997,4551[5]QiangYZetal.JAntimicrobChemother,2002,49:549552[6].,2004,20(2):252256:100021336(2004)0620516202Tilling(,100730):Tilling,,Tilling,,:Tilling;Ecotilling;:Q3:2004209217:(1979),,,E2mail:[e-mail];(1955),,,,,E2mail:[e-mail],,,,,(targetinginducedlocallesionsingenomes,Tilling)TillingPCR,[1],,Tilling,,TillingEcotilling(theTillingtech2niquetosurveynaturalvariationingene),[2],,Ecotilling1.Tilling90,FredHutchinsonClaireM.Mclallum,,DNA,DNA,Tilling[3]2.TillingTilling[4]:DNA,DNAPCR,,,,,,Tilling3.TillingTilling:(1)6152004246CHEMISTRYOFLIFE2004,24(6)TechniquesandMethods©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.