Bio-rad-CFX_Manager-2.0(荧光定量PCR)说明书

CFX96™andCFX384™Real-TimePCRDetectionSystemsInstructionManualCatalog#184-5384#185-5384#184-5096#185-5096Copyright©2008Bio-RadLaboratories,Inc.Reproductioninanyform,eitherprintorelectronic,isprohibitedwithoutwrittenpermissionofBio-RadLaboratories,Inc.Excel,Microsoft,PowerPoint,Windows,andWindowsVistaaretrademarksofMicrosoftCorporation.AdobeandReaderaretrademarksofAdobeSystemsIncorporated.CyisatrademarkofGEHealthcaregroupcompanies.FluorandQuasararetrademarksofBiosearchTechnologies,Inc.SYBRandTexasRedaretrademarksofInvitrogenCorporation.Bio-RadLaboratories,Inc.islicensedbyInvitrogenCorporationtosellreagentscontainingSYBRGreenIforuseinreal-timePCR,forresearchpurposesonly.Otherbrandsorproductnamesaretrademarksoftheirrespectiveholders.LICENSENOTICETOPURCHASERBio-RadReal-timethermalcyclerCFX96orCFX384islicensedreal-timethermalcycler(s)underApplera'sUnitedStatesPatentNo.6,814,934B1foruseinresearchandforallotherfieldsexceptthefieldsofhumandiagnosticsandveterinarydiagnostics.ThisCFX96orCFX384detectionmodule,whencombinedwithaC1000thermalcyclerforwhichtheapplicablereal-timethermalcyclerroyaltyfeehasbeenpaid,constitutesareal-timethermalcyclerlicensedunderU.S.PatentNo.6,814,934andcorrespondingclaimsinanyCanadiancounterpartpatentthereofownedbyAppleraCorporation,forusesolelyinresearchandallappliedfieldsexcepthumanandveterinaryinvitrodiagnostics.TheselicenserightsareeffectiveonlyifthisdetectionmoduleiscombinedwithaBio-Radthermalcyclerforwhichtheapplicablereal-timethermalcyclerroyaltyfeehasbeenpaidandnotwithanyotherthermalcycler.Norightsareconveyedexpressly,byimplicationorestoppeltoanypatentsonreal-timemethods,includingbutnotlimitedto5'nucleaseassays,ortoanypatentclaimingareagentorkit.Forfurtherinformationonpurchasinglicenserights,contacttheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California,94404,USA.ThisproductiscoveredbyoneormoreofthefollowingU.S.patents,theirforeigncounterparts,ortheirforeignpatentspending,ownedbyEppendorfAG:USPatentNos.6,767,512and7,074,367.Norightsareconveyedexpressly,byimplicationorestoppeltoanypatentsonPCRmethods,ortoanypatentclaimingareagentorkitBio-Rad’sHard-Shell®platesarecoveredbyoneormoreofthefollowingU.S.patents,patentspending,ortheirforeigncounterparts,ownedbyEppendorfAG:USPatentNos.7,347,977,6,340,589,6,528,302andUSapplication2007246858,20080084004,20030180192and20060120927iiBio-RadResourcesBio-Radprovidesmanyresourcesforscientists.ThefollowingwebsitescontainusefulinformationaboutrunningPCRandreal-timePCRexperiments:•GeneExpressionGateway()ThissiteprovidesrichtechnicalresourcesonawidevarietyofmethodsandapplicationsrelatedtoPCR,real-timePCR,andgeneexpression•LifeScienceResearchwebsite(discover.bio-rad.com)Thissiteincludeslinkstotechnicalnotes,manuals,productinformation,andtechnicalsupport.Table1listsotherBio-Radresourcesandhowtolocatewhatyouneed:WritingConventionsUsedinthisManualThismanualexplainshowtosafelysetupandoperatetheCFX96systemortheCFX384system.ThemanualusesthewritingconventionslistedinTable2.ForinformationaboutsafetylabelsusedinthismanualandontheCFX96systemortheCFX384system,see,“SafetyandRegulatoryCompliance”onpageiii.Table1.Bio-RadresourcesResourceHowtoContactLocalBio-RadLaboratoriesrepresentativesFindlocalinformationandcontactsontheBio-Radwebsitebyselectingyourcountryonthehomepage().FindthenearestinternationalofficelistedonthebackofthismanualTechnicalnotesandliteratureGototheBio-Radwebsite()orGeneExpressionGateway().TypeasearchtermintheSearchboxandselectLiteraturetofindlinkstotechnicalnotes,manuals,andotherliteratureTechnicalspecialistsBio-Rad’sTechnicalSupportdepartmentisstaffedwithexperiencedscientiststoprovidecustomerswithpracticalandexpertsolutionsTofindlocaltechnicalsupportonthephone,contactyournearestBio-Radoffice.FortechnicalsupportintheUnitedStatesandCanada,call1-800-424-6723(toll-freephone),andselectthetechnicalsupportoptionTable2.ConventionsusedinthismanualConventionMeaningTIP:Provideshelpfulinformationandinstructions,includinginformationexplainedinfurtherdetailelsewhereinthismanualNOTE:Providesimportantinformation,includinginformationexplainedinfurtherdetailelsewhereinthismanualWARNING!Explainsveryimportantinformationaboutsomethingthatmightdamagetheresearcher,damageaninstrument,orcausedatalossXYSelectXandthenselectYfromatoolbar,menuorsoftwarewindowCFX96andCFX384SystemsManualiiiSafetyandRegulatoryComplianceForsafeoperationoftheCFX96systemortheCFX384system,westronglyrecommendthatyoufollowthesafetyspecificationslistedinthissectionandthroughoutthismanual.SafetyWarningLabelsWarninglabelspostedontheinstrumentandinthismanualwarnyouaboutsourcesofinjuryorharm.RefertoTable3toreviewthemeaningofeachsafetywarninglabel.InstrumentSafetyWarningsThewarninglabelsshowninTable4alsodisplayontheinstrument,andreferdirectlytothesafeuseoftheCFX96systemortheCFX384system.NOTE:ForinformationabouttheC1000™thermalcycler,refertotheC1000thermalcyclerinstructionmanual.Table3.Meaningofsafetywar