ReferencenumbersISO11866-2:2005(E)IDF170-2:2005(E)©ISOandIDF2005INTERNATIONALSTANDARDISO11866-2IDF170-2Secondedition2005-12-01Milkandmilkproducts—EnumerationofpresumptiveEscherichiacoli—Part2:Colony-counttechniqueat44°CusingmembranesLaitetproduitslaitiers—Dénombrementd'Escherichiacoliprésumés—Partie2:Techniqueparcomptagedescoloniesobtenuessurmembranesà44°CCopyrightInternationalOrganizationforStandardizationReproducedbyIHSunderlicensewithISONotforResaleNoreproductionornetworkingpermittedwithoutlicensefromIHS--`,,```,,,,````-`-`,,`,,`,`,,`---ISO11866-2:2005(E)IDF170-2:2005(E)PDFdisclaimerThisPDFfilemaycontainembeddedtypefaces.InaccordancewithAdobe'slicensingpolicy,thisfilemaybeprintedorviewedbutshallnotbeeditedunlessthetypefaceswhichareembeddedarelicensedtoandinstalledonthecomputerperformingtheediting.Indownloadingthisfile,partiesacceptthereintheresponsibilityofnotinfringingAdobe'slicensingpolicy.NeithertheISOCentralSecretariatnortheIDFacceptsanyliabilityinthisarea.AdobeisatrademarkofAdobeSystemsIncorporated.DetailsofthesoftwareproductsusedtocreatethisPDFfilecanbefoundintheGeneralInforelativetothefile;thePDF-creationparameterswereoptimizedforprinting.EverycarehasbeentakentoensurethatthefileissuitableforusebyISOmemberbodiesandIDFnationalcommittees.Intheunlikelyeventthataproblemrelatingtoitisfound,pleaseinformtheISOCentralSecretariatattheaddressgivenbelow.©ISOandIDF2005Allrightsreserved.Unlessotherwisespecified,nopartofthispublicationmaybereproducedorutilizedinanyformorbyanymeans,electronicormechanical,includingphotocopyingandmicrofilm,withoutpermissioninwritingfromeitherISOorIDFattherespectiveaddressbelow.ISOcopyrightofficeInternationalDairyFederationCasepostale56•CH-1211Geneva20DiamantBuilding•BoulevardAugusteReyers80•B-1030BrusselsTel.+41227490111Tel.+3227339888Fax+41227490947Fax+3227330413E-mailcopyright@iso.orgE-mailinfo@fil-idf.orgWeb©ISOandIDF2005–AllrightsreservedCopyrightInternationalOrganizationforStandardizationReproducedbyIHSunderlicensewithISONotforResaleNoreproductionornetworkingpermittedwithoutlicensefromIHS--`,,```,,,,````-`-`,,`,,`,`,,`---ISO11866-2:2005(E)IDF170-2:2005(E)©ISOandIDF2005–AllrightsreservediiiForewordISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISOmemberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhastherighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnon-governmental,inliaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnicalCommission(IEC)onallmattersofelectrotechnicalstandardization.InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part2.ThemaintaskoftechnicalcommitteesistoprepareInternationalStandards.DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting.PublicationasanInternationalStandardrequiresapprovalbyatleast75%ofthememberbodiescastingavote.Attentionisdrawntothepossibilitythatsomeoftheelementsofthisdocumentmaybethesubjectofpatentrights.ISOshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights.ISO11866-2⎪IDF170-2waspreparedbyTechnicalCommitteeISO/TC34,Foodproducts,SubcommitteeSC5,Milkandmilkproducts,andtheInternationalDairyFederation(IDF).ItisbeingpublishedjointlybyISOandIDF.ThiseditionofISO11866-2⎪IDF170-2cancelsandreplacesISO11866-3:1997,ofwhichitconstitutesaminorrevision.ISO11866-1:1997hasbeencancelledandreplacedbyISO7251:2005,Microbiologyoffoodandanimalfeedingstuffs—HorizontalmethodforthedetectionandenumerationofpresumptiveEscherichiacoli—Mostprobablenumbertechnique.ISO11866⎪IDF170consistsofthefollowingparts,underthegeneraltitleMilkandmilkproducts—EnumerationofpresumptiveEscherichiacoli:⎯Part1:Mostprobablenumbertechniqueusing4-methylumbelliferyl-β-D-glucuronide(MUG)⎯Part2:Colony-counttechniqueat44°CusingmembranesCopyrightInternationalOrganizationforStandardizationReproducedbyIHSunderlicensewithISONotforResaleNoreproductionornetworkingpermittedwithoutlicensefromIHS--`,,```,,,,````-`-`,,`,,`,`,,`---ISO11866-2:2005(E)IDF170-2:2005(E)iv©ISOandIDF2005–AllrightsreservedForewordIDF(theInternationalDairyFederation)isaworldwidefederationofthedairysectorwithaNationalCommitteeineverymembercountry.EveryNationalCommitteehastherighttoberepresentedontheIDFStandingCommitteescarryingoutthetechnicalwork.IDFcollaborateswithISOinthedevelopmentofstandardmethodsofanalysisandsamplingformilkandmilkproducts.DraftInternationalStandardsadoptedbytheActionTeamsandStandingCommitteesarecirculatedtotheNationalCommitteesforvoting.PublicationasanInternationalStandardrequiresapprovalbyatleast50%oftheIDFNationalCommitteescastingavote.Attentionisdrawntothepossibilitythatsomeoftheelementsofthisdocumentmaybethesubjectofpatentrights.IDFshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights.ISO11866-2⎪IDF170-2wasp