1株好氧反硝化芽孢杆菌的脱氮特性研究

2320095JournalofHydroecologyVo.l2,No.3May,2009:2008-06-11:,1983,,,E-mai:[e-mail]1李兵,林炜铁(华南理工大学生物科学与工程学院,广东广州510006):D5,,,0.2mol/L36epH7.05~7mg/L,,:;;:Q939.1:A:1674-3075(2009)03-0048-05,,(JoongKyunKimeta,l2005),,,(Pseudomonas)(Alcaligenes)(Paracoccus)(Bacillus),,,,,,,,,,,,D5,,11.11.1.1菌种D5,1.1.2培养基LB:0.5gNaCl1g1g100mL,pH7.2~7.4LBNaNO2,:1gNaNO30.1g100mL,pH6~71.2,2%50mLLB,32e180r/min,2hOD600,LB1.3D51.3.1不同碳源实验LB,0.1mol/L5:,LBD5,32e200r/min,12h1.3.2不同碳源浓度实验LB,,0.020.040.10.20.4mol/L,LBD5,32e200r/min,12h24h1.3.3不同温度条件下的反硝化特性LB,D5,2730333639e,200r/min,12h1.3.4不同初始pH下的反硝化特性pH,pH4.05.06107.08.0,33.3mg/L,D5,12h1.3.5不同溶氧下的反硝化特性LB,D5,120150180210240r/min,30e,12h1.4(NO3--N):;(NO2--N):N-(1-)-;:;:(-)/@,mg/(L#h)3,22.11,4h,14h,1D5Fig.1BacillusD5growthcurve2.2D52.2.1不同碳源对反硝化速率的影响2,,,D5,2.2.2不同碳源浓度对反硝化速率的影响3,,,2.2.3不同温度对反硝化速率的影响4,36eD5,,2.2.4不同初始pH对反硝化速率的影响5,pH4.0,D5,;pH7.05pHFig.5EffectofdifferentinitialpHondenitrificationrate2.2.5不同溶氧对反硝化能力的影响6492009年第3期李兵等,1株好氧反硝化芽孢杆菌的脱氮特性研究,150r/min,D5150r/minDO5~7mg/L,6Fig.6Effectofdifferentrotationspeedondenitrificationrate33.1D5,,,,(TavaresPeta,l2006)NO3-yNO2-yNOyN2OyN2,1,2,,Bell(1990),,,,Gupta(1997)(Thiosphaerapantotropha),,,3.2D51D5,4h,14hOD600,D51stopt1.5,,y=4.34/[1+119.55exp(-0.48t)],R2=0.98,,,,,D5,(2005),,3.3D53.3.1不同碳源对反硝化速率的影响D5,,,,,,3.3.2不同碳源浓度对反硝化速率的影响2080,Robertson(1983),,,,D50.2mol/L0.4mol/L,,,0.2mol/L3.3.3不同温度对反硝化速率的影响,20~40eD527e,30e,3.3.4不同初始pH对反硝化速率的影响,pH6.5~7.5,pHpH,3.3.5不同溶氧对反硝化能力的影响(KlangduenPochana&JurgKeller,1999),,,,(O2NO2-,NO3-)50第2卷第3期水生态学杂志2009年5月,O2NO2-NO3-,,,NO2-NO3-,N2,,,3mg/L,4~8mg/L,D53.3.6D5反硝化特性总结D5,,,,,0.2mol/L;36e,30e;pH7.0,pHpH;150r/min5~7mg/L,210r/min,(2005)1,2mg/L,,D55~7mg/L,,(Bacilluslicheniformis)(Bacillussubtilis)(Bacilluscereus)(JoongKyunKimeta,l2005)(Bacillussphaericus)(Bacillusfirmus)(FanetLFa,l2006),,,D5(2006)1,6.74mg/LLB,0.85mg/(L#h),D530mg/L2.22mg/(L#h),D5,:,,,.2005.X31[J].:,33(7):42-46.,,.2005.[J].,25(3):77-81.,.2006.[J].,36(5):28-32.BellLC,RichardsonDJ,FergusonSJ.1990.Periplasmicandmembrane-boundnitratereductasesinThiosphaerapantotro-pha[J].FEBSLetters,(265):85-87.GuptaAB.1997.Thiosphaerapantotropha:asulphurbacter-iumcapableofsimultaneousheterotrophicnitrificationandaerobicdenitrification[J].EnzymeandMicrobialTechno-logy,(21):589-595.JoongKyunKim,KyoungJooPark,KyoungSookCho,eta.l2005.Aerobicnitrification-denitrificationbyheterotrophicBacillusstrains[J].BioresourceTechnology,(96):1897-1906.KlangduenPochana,JurgKeller.1999.Modeldevelopmentforsimultaneousnitrificationanddenitrification[J].WaterSc-ienceandTechnology,39(1):235-242.FanLF,ShiehWY,WuWF,eta.l2006.Distributionofn-itrogenousnutrientsanddenitrifiersstrainsinestuarinesed-imentprofilesoftheTanshuiRiver,northernTaiwan[J].Estuarine,CoastalandShelfScience,(69):543-553.TavaresP,PereiraAS,MouraJJG.2006.Metalloenzymesofthedenitrificationpathway[J].JournalofInorganicBio-chemistry,(100):2087-2100.RobertsonLA,KuenenJG.1983.Thiosphaerapantotrophagen.nov.sp.nov.,afacultativelyanaerobic,facultative-lyautotrophicsulphurbacterium[J].JoumalofGeneralMicrobiology,129(8):2847-2855.(责任编辑杨春艳)512009年第3期李兵等,1株好氧反硝化芽孢杆菌的脱氮特性研究StudyonAerobicDenitrificationCharacteristicsofBacillusStrainD5LIBing,LINWe-itie(CollegeofBiologicalScienceandBioengineering,SouthChinaUniversityofTechnology,Guangzhou510006,China)Abstract:ABacillusstrainwhichhadhighlyeffctivedenitrificationastheoriginalstrainwasusedtostudythecharacteristicsofgrowthandeliminationnitrogenunderaerobicconditions.Theresultsshowedthatithadhighde-nitrifyabilityeveninconditionwithhigh-concentrationofnitrite.Andwhensodiumacetateplayedascarbonsource,BacillusD5hadhighestdenitrificationeffciency.Whentheconcentrationofcarbonsourcewas0.2mol/L,theinitialpHat7.0,dissolvedoxygenat5~7mg/L,and36e,BacillusD5possessedtheoptimumdenitrificationactivity.Ascomparedwithotherdenitrificationbacteriumintherepor,tbacillusstrainhadthesuperioroxygen-re-sistance,andhigherdenitrificationrate.Keywords:Bacillus;Aerobicdenitrification;Nitrite52第2卷第3期水生态学杂志2009年5月