2株溶藻菌培养条件优化及溶藻特性研究

281Vol．28No．120151JournalofYantaiUniversityNaturalScienceandEngineeringEditionJan．20151004-8820201501-0024-06doi10．13951/j．cnki．37-1213/[e-mail]、．221009522．2、．Q31A．、、、、1－3．216SrDNAYT．2、．11.1MicrocystisaeruginosaFACHB469．BG－11．Y4Achromobactersp．GenBankEF617310T5BacillusCohnGenBankFJ815201．5－6．1.21.2.1250mL100mLBG－115mL10mL6000r/min10min．、pH7、8．9－1020、25、30℃2000、2500、30001xpH6.0、7.0、8.011－123．3L93491．312∶12．1219Tab．1Environmentalfactorsof9groupsforMicrocystisaeruginosa/℃/lxpH12020006．022525006．033030006．042520006．053025007．062030008．073020008．082025008．092530008．01.2.2YT．、pH13－152．．22．pH5.5、pH8.52、2A663TY．42TpH5．5．TA663TT．YT2pH5．5Y．22Tab．2Culturecondionof2strains/℃/r·min－112016022010032816042810022.131A6634d124d．24．1．1.81.61.41.210.80.60.40.20A66315913172124组1组2组3组4组5组6组7组8组9t/d1Fig．1EffectsofcultureconditionsongrowthofMicrocystisaeruginosa1925℃、3000lx、pH8.024A6631.86.502×107/mL12.5%．2.2“1．2．2”A60022．2aT3pH5.5、28℃、160r/min．T26A6001.21、2、431.8%、59.0%39.6%．2bY4pH5.5、28℃、100r/min28A6000.85．52281.41.210.80.60.40.2012162022242628A600组1组2组3组4t/h(a)T菌株12162022242628A600组1组2组3组4t/h10.90.80.70.60.50.40.30.20.10(b)Y菌株2Fig．2Growthcurveofbacterialstrain2.321618100mLBG－115mL10mL6000r/min10min、T、Y4、6、8mL33．a1222．212、．T、Y．23．9876543210-12468101214测量时间/d叶绿素a含量/(mg·L-1)CK4mL6mL8mL9876543210-12468101214测量时间/d叶绿素a含量/(mg·L-1)CK4mL6mL8mL(a)T菌株（b）Y菌株3Fig．3Influenceofbacterialamountsonthealgae-lysingeffect3a17－18．226mL8mL241012～1499%414．、．62122.42XJD332a2．31∶319100mL3325℃3000lx．37d．28mL32．2dXJDa12d．2d112d．4．4a、bXJD3a22aa3．4c312d23．．T、Y2XJD2．2.52250mL30BG－11100mL5mL10mL6000r/min10min．1535T、、121℃0.2μm12000r/min、315YT．a．5．5ab121℃212000r/min、2测量时间/d121086420细胞密度/（×106/mL）加T菌株加Y菌株CK2468101276543210加入Y菌株加入T菌株CK叶绿素a含量/（mg·L-1）24681012测量时间/d（a）斜生栅藻加入Y菌株加入T菌株CK叶绿素a含量/（mg·L-1）24681012测量时间/d2.521.510.50（b）沙漠绿藻(c)小新月菱形藻42Fig．4Effectsoftwostrainsongrowthofmicroalgae72289876543210-124681012测量时间/d叶绿素a含量/(mg·L-1)菌体浸提液菌体菌液菌液上清高热处理（a）T菌株9876543210-124681012测量时间/d叶绿素a含量/(mg·L-1)菌体浸提液菌体菌液菌液上清高热处理（b）Y菌株52Fig．5Lyticeffectsofbacterialiquidsafterbeingtreatedwithdifferentmethods．220．2．2．22SOD、MDA、21－22．3125℃3000lxpH8.0．2TpH5.528℃、160r/minYpH5.528℃、100r/min．3222．4299%14．2．52．1．———J．1996202173－181．2．J．2007615－18．3．J．2008354－58．4．、J．2008579－83．5．D．2010．6．J．20001131647－651．7WrangstadhMSzewzykUOstlingJetal．Starvation-specificformationofaperipheralexopolysaccharidebyamarinePseudomonasstrainS9J．AppliedandEnvironmentalMicro-biology19905672065－2072．8．M．3．2004．9．J．201030625－28．10BracherAUTilzerMM．UnderwaterlightfieldandphytoplanktonabsorbanceindifferentsurfacewatermassesoftheAtlanticsectoroftheSouthernOceanJ．PolarBiology2001249687－696．11ShiShunyuTangDongshanLiuYongding．EffectsofanalgicidalbacteriumPseudomonasmendocinaonthegrowthand8212antioxidantsystemofAphanizomenonflos-aquaeJ．CurrentMicrobiology2009592107－112．12GrantJJLoakeGJ．Ｒoleofreactiveoxygenintermedi-atesandcognateredoxsignalingindiseaseresistanceJ．PlantPhysiology2000124121－30．13．D．200925－26．14．pHJ．2008392189－194．15NeillSDesikanＲHancockJ．Hydrogenperoxidesig-nallingJ．CurrentOpinioninPlantBiology200255388－395．16SuJianqiangYangXiaoruZhengTianlingetal．Isola-tionandcharacterizationofamarinealgicidalbacteriumagainstthetoxicdinoflagellateAlexandriumtamarenseJ．HarmfulAl-gae200766799－810．17．B5J．2003394489－493．18．J．20042108－110．19．J．20093561－64．20．J．2008726－31．21．J．2005294456－461．22．———J．2011101－6．CultureConditionOptimizationandAlgicidalPropertiesofTwoStrainsofAlgicidalBacteriaMADi-diZHAOGeng-maoWANGChang-haiCollegeofＲesourcesandEnvironmentalScienceNanjingAgriculturalUniversityJiangsuKeyLaboratoryofMarineBiologyNanjing210095ChinaAbstractThecultureconditionsoftwostrainsofalgicidalbacteriawhichisolatedfromtwoeutrophicpondsinShandongHuangdaoandJiangsuTaihuareoptimized．TheinhibitoryeffectofthetwostrainsonthegrowthofMi-crocystisaeruginosaareexplored．Theexperimentalresultsshowthatthetwostrainscanproducebiologicaldegrada-tionbycoagulationalgaecellscleavageofmicroalgaecellsandextracellularsecretionofactivesubstancestokillthealgaecells．BesidesthekillingeffectofextracellularsubstancesoftwostrainsonMicrocystisaeruginosaarespecificity．KeywordsalgicidalbacteriaMicrocystisaeruginosacultureconditionoptimizaionactivesubstance92